Recombinant construct P{UAS-rok.CAT}

General Information
Symbol	P{UAS-rok.CAT}	FlyBase ID	FBtp0014097
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	3 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Winter et al., 2001)
Uses	CV term Qualifiers & info Reference binary system (targeted) rok (Winter et al., 2001) characterization rok (Winter et al., 2001)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference binary component (Winter et al., 2001) selectable marker (Winter et al., 2001)
Component Alleles
Allele	rok^CAT.Scer\UAS
Reference(s)	(Winter et al., 2001, Yan et al., 2009, Billuart et al., 2001, Warner and Longmore, 2009, Lee and Treisman, 2004, Echard and O'Farrell, 2003, Ninov et al., 2007, Chung et al., 2007, Neisch et al., 2010, Sun et al., 2011, Xu et al., 2008, Corrigall et al., 2007, Okumura et al., 2010)
Molecular data	Construct: Scer\UAS regulatory sequences drive expression of the catalytic domain (amino acids 1 to 454) of rok. (Winter et al., 2001)
Phenotypic class	cell polarity defective \| somatic clone, with Scer\GAL4^{Scer\FRT.Act5C} (Sun et al., 2011) lethal \| pupal stage, with Scer\GAL4^da.G32 (Lee and Treisman, 2004) neuroanatomy defective, with Scer\GAL4^ey-OK107 (Billuart et al., 2001)
Phenotype manifest in	embryonic/larval epidermis, with Scer\GAL4^NP0056 (Ninov et al., 2007) mushroom body vertical lobe, with Scer\GAL4^ey-OK107 (Billuart et al., 2001) oocyte \| somatic clone, with Scer\GAL4^{Scer\FRT.Act5C} (Sun et al., 2011)
Other information

Allele	w^+mC
Reference(s)	(Winter et al., 2001)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 3 )
Bloomington	6667 P{UAS-rok.CAT}2.1, y¹ w^* 6668 y¹ w^; P{UAS-rok.CAT}7.1 6669 y¹ w^; P{UAS-rok.CAT}3.1
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	Drok-CAT (Billuart et al., 2001) P{UAS-rok.CAT} (FlyBase, 1996-) Rok.CAT (Sun et al., 2011) UAS-Drok-CAT (Echard and O'Farrell, 2003, Winter et al., 2001, Chung et al., 2007, Okumura et al., 2010) UAS-Rok^CAT (Ninov et al., 2007) UAS-rokCAT (Lee and Treisman, 2004, Neisch et al., 2010) UAS-Rok-CAT (Yan et al., 2009, Sun et al., 2011) UAS-rok-CAT (Xu et al., 2008) UAS-ROKCAT (Corrigall et al., 2007) UAS-Rok-catalytic domain (Warner and Longmore, 2009)
Secondary FlyBase IDs

References ( 13 )
Research paper	Sun et al., 2011, Development 138(10): 1991--2001 Regulation of somatic myosin activity by Protein Phosphatase 1{beta} controls Drosophila oocyte polarization. [FBrf0213586] Neisch et al., 2010, J. Cell Biol. 189(2): 311--323 Rho1 regulates apoptosis via activation of the JNK signaling pathway at the plasma membrane. [FBrf0210554] Okumura et al., 2010, Dev. Biol. 344(2): 693--706 Left-right asymmetric morphogenesis of the anterior midgut depends on the activation of a non-muscle myosin II in Drosophila. [FBrf0211383] Warner and Longmore, 2009, J. Cell Biol. 185(6): 1111--1125 Distinct functions for Rho1 in maintaining adherens junctions and apical tension in remodeling epithelia. [FBrf0208183] Yan et al., 2009, Dev. Biol. 333(1): 186--199 Rho1 has multiple functions in Drosophila wing planar polarity. [FBrf0208582] Xu et al., 2008, Dev. Biol. 321(1): 88--100 Rho GTPase controls invagination and cohesive migration of the Drosophila salivary gland through Crumbs and Rho-kinase. [FBrf0206881] Chung et al., 2007, Genetics 176(2): 891--903 The balance between the novel protein target of wingless and the Drosophila Rho-associated kinase pathway regulates planar cell polarity in the Drosophila wing. [FBrf0202398] Corrigall et al., 2007, Dev. Cell 13(5): 730--742 Hedgehog Signaling Is a Principal Inducer of Myosin-II-Driven Cell Ingression in Drosophila Epithelia. [FBrf0202245] Ninov et al., 2007, Development 134(2): 367--379 Extrinsic and intrinsic mechanisms directing epithelial cell sheet replacement during Drosophila metamorphosis. [FBrf0194691] Lee and Treisman, 2004, Mol. Biol. Cell 15(7): 3285--3295 Excessive myosin activity in Mbs mutants causes photoreceptor movement out of the Drosophila eye disc epithelium. [FBrf0180500] Echard and O'Farrell, 2003, Curr. Biol. 13(5): 373--383 The degradation of two mitotic cyclins contributes to the timing of cytokinesis. [FBrf0158812] Billuart et al., 2001, Cell 107(2): 195--207 Regulating axon branch stability. The role of p190 RhoGAP in repressing a retraction signaling pathway. [FBrf0139610] Winter et al., 2001, Cell 105(1): 81--91 Drosophila Rho-associated kinase (Drok) links Frizzled-mediated planar cell polarity signaling to the actin cytoskeleton. [FBrf0135688]