Recombinant construct P{UAS-Hsap\KCNJ2.EGFP}

General Information
Symbol	P{UAS-Hsap\KCNJ2.EGFP}	FlyBase ID	FBtp0014166
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	2 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02	Controlled Vocabulary Terms embryonic/larval olfactory receptor neuron projection neuron
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Baines et al., 2001)
Uses	CV term Qualifiers & info Reference binary system (targeted) Hsap\KCNJ2 (Baines et al., 2001) characterization Hsap\KCNJ2 (Baines et al., 2001) reporter construct vital \| Hsap\KCNJ2 (Baines et al., 2001)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference binary component (Baines et al., 2001) molecular tag (Baines et al., 2001) selectable marker (Baines et al., 2001)
Component Alleles
Allele	Hsap\KCNJ2^{Scer\UAS.T:Avic\GFP-EGFP}
Reference(s)	(Baines et al., 2001, Nitabach et al., 2002, Kueppers et al., 2003, Luan et al., 2006, Nitabach et al., 2006, Malpel et al., 2002, Paradis et al., 2001, Hardie et al., 2002, Olofsson and Page, 2005, Larsson et al., 2004, Tsai et al., 2004, Hardie et al., 2004, Hardie et al., 2001, Fischler et al., 2007, Gordon and Scott, 2009, Joiner et al., 2006, Hardie et al., 2007, Suster et al., 2003, King et al., 2011, Keene et al., 2011, Yuan et al., 2011, Depetris-Chauvin et al., 2011, Yang et al., 2008, Hergarden et al., 2012, Koon et al., 2011, Dankert et al., 2009, Wang and Anderson, 2010, Peabody et al., 2008, Cognigni et al., 2011, Roy et al., 2007, López-Arias et al., 2011, Bang et al., 2011, Ofstad et al., 2011, Claridge-Chang et al., 2009, Sadaf et al., 2012, Prieto-Godino et al., 2012, Schneider et al., 2012, Tayler et al., 2012, Tayler et al., 2012)
Molecular data	Construct: Scer\UAS regulatory sequences drive expression of Hsap\KCNJ2 tagged with T:Avic\GFP^EGFP (EGFP). (Baines et al., 2001)
Phenotypic class	behavior defective, with Scer\GAL4^Tdc2.PC (Dankert et al., 2009) behavior defective \| RU486 conditional, with Scer\GAL4^{Mef2.247.Switch} (Joiner et al., 2006) chemical resistant, with Scer\GAL4^P2.4.Pdf (Tsai et al., 2004) chemosensitive behavior defective, with Scer\GAL4^Tdc2.PC (Schneider et al., 2012) chemosensitive behavior defective \| adult stage, with Scer\GAL4^4.64 (King et al., 2011) chemosensitive behavior defective \| adult stage, with Scer\GAL4^10.229 (King et al., 2011) chemosensitive behavior defective \| adult stage, with Scer\GAL4^17d (King et al., 2011) chemosensitive behavior defective \| adult stage, with Scer\GAL4^Tdc2.PC (Hardie et al., 2007) female sterile, with Scer\GAL4^Ilp7.PY (Yang et al., 2008) female sterile, with Scer\GAL4^Tdc2.PC (Hardie et al., 2007) flight defective, with Scer\GAL4^Trh.PB (Sadaf et al., 2012) lethal, with Scer\GAL4^DAT.PL (Bang et al., 2011) lethal, with Scer\GAL4^elav-C155 (Nitabach et al., 2002) lethal, with Scer\GAL4^GMR.PF (Malpel et al., 2002) lethal, with Scer\GAL4^ple.PF (Bang et al., 2011) lethal \| larval stage, with Scer\GAL4^dimm-929 (Luan et al., 2006) lethal \| larval stage, with Scer\GAL4^dimm-929, Scer\GAL80^Ccap.PL (Luan et al., 2006) locomotor behavior defective \| adult stage, with Scer\GAL4^238Y (King et al., 2011) locomotor behavior defective \| adult stage, with Scer\GAL4^Tdc2.PC (Hardie et al., 2007) locomotor behavior defective \| adult stage \| heat sensitive, with Scer\GAL4^Ddc.HL9 (Claridge-Chang et al., 2009) locomotor behavior defective \| adult stage \| heat sensitive, with Scer\GAL4^ple.PF (Claridge-Chang et al., 2009) locomotor behavior defective \| third instar larval stage, with Scer\GAL4^dimm-929 (Suster et al., 2003) locomotor rhythm defective, with Scer\GAL4^P2.4.Pdf (Nitabach et al., 2002, Nitabach et al., 2006) locomotor rhythm defective \| RU486 conditional, with Scer\GAL4^Pdf.Switch1a (Depetris-Chauvin et al., 2011) male sterile, with Scer\GAL4^Crz.391 (Tayler et al., 2012) male sterile, with Scer\GAL4^CrzR.3.5 (Tayler et al., 2012) male sterile, with Scer\GAL4^Trh.1.0 (Tayler et al., 2012) mating defective, with Scer\GAL4^Crz.391 (Tayler et al., 2012) memory defective \| adult stage \| heat sensitive, with Scer\GAL4^ple.PF (Claridge-Chang et al., 2009) neuroanatomy defective, with Scer\GAL4^GMR.PF (Malpel et al., 2002) neuroanatomy defective, with Scer\GAL4^αTub84B.PP (Roy et al., 2007) neuroanatomy defective \| first instar larval stage, with Scer\GAL4^Lim3b (Prieto-Godino et al., 2012) neuroanatomy defective \| larval stage, with Scer\GAL4^Orco.PU (Prieto-Godino et al., 2012) neuroanatomy defective \| RU486 conditional, with Scer\GAL4^Pdf.Switch1a (Depetris-Chauvin et al., 2011) neuroanatomy defective \| third instar larval stage (Koon et al., 2011) neuroanatomy defective \| third instar larval stage, with Scer\GAL4^Mhc.PW (Paradis et al., 2001) neurophysiology defective, with Scer\GAL4^eve.RRC (Baines et al., 2001) neurophysiology defective, with Scer\GAL4^Trh.PB (Sadaf et al., 2012) neurophysiology defective, with Scer\GAL4^unspecified (Yuan et al., 2011) neurophysiology defective \| larval stage, with Scer\GAL4^Orco.PU (Prieto-Godino et al., 2012) neurophysiology defective \| RU486 conditional, with Scer\GAL4^Pdf.Switch1a (Depetris-Chauvin et al., 2011) neurophysiology defective \| third instar larval stage, with Scer\GAL4^Mhc.PW (Paradis et al., 2001) paralytic \| embryonic stage, with Scer\GAL4^elav-C155 (Olofsson and Page, 2005) partially lethal - majority die \| pupal stage, with Scer\GAL4^burs.PP (Peabody et al., 2008) partially lethal - majority die \| pupal stage \| heat sensitive, with Scer\GAL4^burs.PP, Scer\GAL80^ts.αTub84B (Peabody et al., 2008) phototaxis defective, with Scer\GAL4^cry.PZ (Keene et al., 2011) phototaxis defective, with Scer\GAL4^tim.PE (Keene et al., 2011) phototaxis defective, with Scer\GAL4^tim.PE, Scer\GAL80^cry.PS (Keene et al., 2011) phototaxis defective, with Scer\GAL4^tim.PE, Scer\GAL80^Pdf.PS (Keene et al., 2011) short lived, with Scer\GAL4^Lk.2.2 (López-Arias et al., 2011) smell perception defective, with Scer\GAL4^ey-OK107 (Ofstad et al., 2011) smell perception defective, with Scer\GAL4^GMR9A11 (Ofstad et al., 2011) smell perception defective, with Scer\GAL4^GMR10B08 (Ofstad et al., 2011) smell perception defective, with Scer\GAL4^GMR67B04 (Ofstad et al., 2011) starvation stress response defective, with Scer\GAL4^Ast.2.1 (Hergarden et al., 2012) stress response defective, with Scer\GAL4^Lk.2.2 (López-Arias et al., 2011) taste perception defective, with Scer\GAL4^E409 (Fischler et al., 2007) taste perception defective, with Scer\GAL4^Lk.2.2 (López-Arias et al., 2011) thermotaxis behavior defective \| adult stage \| heat sensitive, with Scer\GAL4^DAT.PL, Scer\GAL80^ts.αTub84B (Bang et al., 2011) thermotaxis behavior defective \| adult stage \| heat sensitive, with Scer\GAL4^ple.PF, Scer\GAL80^ts.αTub84B (Bang et al., 2011) visible \| heat sensitive, with Scer\GAL4^burs.PP, Scer\GAL80^ts.αTub84B (Peabody et al., 2008) visual behavior defective, with Scer\GAL4^GMR15B07 (Ofstad et al., 2011) visual behavior defective, with Scer\GAL4^GMR28D01 (Ofstad et al., 2011)
Phenotype manifest in	A1-7 dorsal acute muscle 1, with Scer\GAL4^eve.RRC (Baines et al., 2001) aCC neuron, with Scer\GAL4^eve.RRC (Baines et al., 2001) Bolwig nerve, with Scer\GAL4^GMR.PF (Malpel et al., 2002) CSD interneuron, with Scer\GAL4^αTub84B.PP (Roy et al., 2007) embryonic/larval muscle system, with Scer\GAL4^Mhc.PW (Paradis et al., 2001) embryonic/larval neuromuscular junction \| first instar larval stage \| heat sensitive, with Scer\GAL4^Tdc2.PC, Scer\GAL80^ts.αTub84B (Koon et al., 2011) embryonic/larval neuromuscular junction \| third instar larval stage, with Scer\GAL4^Tdc2.PC (Koon et al., 2011) embryonic/larval olfactory receptor neuron, with Scer\GAL4^Orco.PU (Prieto-Godino et al., 2012) embryonic/larval olfactory receptor neuron \| first instar larval stage, with Scer\GAL4^Lim3b (Prieto-Godino et al., 2012) larval head, with Scer\GAL4^Ccap.PP (Luan et al., 2006) larval LN period neuron, with Scer\GAL4^GMR.PF (Malpel et al., 2002) leg, with Scer\GAL4^Ccap.PP (Luan et al., 2006) l-LNv neuron \| RU486 conditional, with Scer\GAL4^Pdf.Switch1a (Depetris-Chauvin et al., 2011) LNv neuron \| third instar larval stage, with Scer\GAL4^unspecified (Yuan et al., 2011) Malpighian tubule, with Scer\GAL4^Lk.2.2 (López-Arias et al., 2011) multidendritic neuron, with Scer\GAL4^Tdc2.PC (Koon et al., 2011) multidendritic neuron \| heat sensitive, with Scer\GAL4^Tdc2.PC, Scer\GAL80^ts.αTub84B (Koon et al., 2011) NMJ bouton, with Scer\GAL4^Mhc.PW (Paradis et al., 2001) photoreceptor cell, with Scer\GAL4^ninaE.PT (Hardie et al., 2001) proboscis, with Scer\GAL4^Lk.2.2 (López-Arias et al., 2011) projection neuron \| larval stage, with Scer\GAL4^Orco.PU (Prieto-Godino et al., 2012) protocerebrum \| RU486 conditional, with Scer\GAL4^Pdf.Switch1a (Depetris-Chauvin et al., 2011) type I bouton, with Scer\GAL4^Tdc2.PC (Koon et al., 2011) type II bouton, with Scer\GAL4^Tdc2.PC (Koon et al., 2011) type II bouton \| first instar larval stage \| heat sensitive, with Scer\GAL4^Tdc2.PC, Scer\GAL80^ts.αTub84B (Koon et al., 2011) ventral nerve cord \| embryonic stage, with Scer\GAL4^elav-C155 (Olofsson and Page, 2005) wing, with Scer\GAL4^Ccap.PP (Luan et al., 2006) wing \| adult stage A1, with Scer\GAL4^burs.PP (Peabody et al., 2008) wing \| adult stage A1 \| heat sensitive, with Scer\GAL4^burs.PP, Scer\GAL80^ts.αTub84B (Peabody et al., 2008)
Other information

Allele	w^+mC
Reference(s)	(Baines et al., 2001)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 4 )
Bloomington	6595 w^; P{UAS-Hsap\KCNJ2.EGFP}7 6596 w^; P{UAS-Hsap\KCNJ2.EGFP}1
Kyoto	108845 w^; P{UAS-Hsap\KCNJ2.EGFP}7 108846 w^; P{UAS-Hsap\KCNJ2.EGFP}1
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	Kir2.1 (Ofstad et al., 2011) Kir2.1-GFP (Kueppers et al., 2003) P{UAS-Hsap\KCNJ2.EGFP} (FlyBase, 1996-) pUAS-EGFP-Kir2.1 (Hardie et al., 2001) pUAS-EGFP-Kir_2.1 (Baines et al., 2001) UAS-eGFP-Kir2.1 (Tayler et al., 2012) UAS-EGFP-Kir2.1 (Roy et al., 2007) UAS-egfp-kir2.1 (Depetris-Chauvin et al., 2011) UAS-K_ir2.1 (Peabody et al., 2008) UAS-Kir2.1 (Olofsson and Page, 2005, Larsson et al., 2004, Tsai et al., 2004, Nitabach et al., 2002, Malpel et al., 2002, Joiner et al., 2006, Tayler et al., 2012, Hergarden et al., 2012, Schneider et al., 2012, Hardie et al., 2007, Claridge-Chang et al., 2009, Fischler et al., 2007, Paradis et al., 2001, Yang et al., 2008, King et al., 2011, Dankert et al., 2009, Wang and Anderson, 2010, Peabody et al., 2008, Prieto-Godino et al., 2012, Cognigni et al., 2011, Bang et al., 2011, Keene et al., 2011, Yuan et al., 2011, Koon et al., 2011) UAS-KIR2.1 (Gordon and Scott, 2009) UASkir2.1 (López-Arias et al., 2011) UASKir2.1 (Sadaf et al., 2012) UAS-Kir2.1-EGFP7 (Luan et al., 2006) UAS-Kir 2.1-Green Fluorescent Protein (Suster et al., 2003) UAS-mKir2.1(III) (Keene et al., 2011)
Secondary FlyBase IDs

References ( 39 )

Generate a list of	All references relating to this transgenic transposon ( 39 )

List References by type	Research paper ( 38 ) Supplementary material ( 1 )
Recent research papers ( 14 )
	Hergarden et al., 2012, Proc. Natl. Acad. Sci. U.S.A. 109(10): 3967--3972 Allatostatin-A neurons inhibit feeding behavior in adult Drosophila. [FBrf0217661] Prieto-Godino et al., 2012, PLoS Biol. 10(10): e1001400 Embryonic origin of olfactory circuitry in Drosophila: contact and activity-mediated interactions pattern connectivity in the antennal lobe. [FBrf0219665] Sadaf et al., 2012, PLoS ONE 7(9): e46405 Synaptic Activity in Serotonergic Neurons Is Required for Air-Puff Stimulated Flight in Drosophila melanogaster. [FBrf0219558] Schneider et al., 2012, PLoS ONE 7(12): e52007 Neuronal basis of innate olfactory attraction to ethanol in Drosophila. [FBrf0220349] Tayler et al., 2012, Proc. Natl. Acad. Sci. U.S.A. 109(50): 20697--20702 A neuropeptide circuit that coordinates sperm transfer and copulation duration in Drosophila. [FBrf0220217] Bang et al., 2011, PLoS Genet. 7(3): e1001346 Dopamine signalling in mushroom bodies regulates temperature-preference behaviour in Drosophila. [FBrf0213392] Cognigni et al., 2011, Cell Metab. 13(1): 92--104 Enteric neurons and systemic signals couple nutritional and reproductive status with intestinal homeostasis. [FBrf0212594] Depetris-Chauvin et al., 2011, Curr. Biol. 21(21): 1783--1793 Adult-specific electrical silencing of pacemaker neurons uncouples molecular clock from circadian outputs. [FBrf0216659] Keene et al., 2011, J. Neurosci. 31(17): 6527--6534 Distinct visual pathways mediate Drosophila larval light avoidance and circadian clock entrainment. [FBrf0213587] King et al., 2011, J. Neurosci. 31(3): 1139--1148 Drosophila tao Controls Mushroom Body Development and Ethanol-Stimulated Behavior through par-1. [FBrf0212818] Koon et al., 2011, Nat. Neurosci. 14(2): 190--199 Autoregulatory and paracrine control of synaptic and behavioral plasticity by octopaminergic signaling. [FBrf0212895] López-Arias et al., 2011, Peptides 32(3): 545--552 Blockade of the release of the neuropeptide leucokinin to determine its possible functions in fly behavior: Chemoreception assays. [FBrf0213007] Ofstad et al., 2011, Nature 474(7350): 204--207 Visual place learning in Drosophila melanogaster. [FBrf0213884] Yuan et al., 2011, Science 333(6048): 1458--1462 Light-induced structural and functional plasticity in Drosophila larval visual system. [FBrf0215276] Show all research papers ...