Recombinant construct P{arm-fz.GFP}

General Information
Symbol	P{arm-fz.GFP}	FlyBase ID	FBtp0014592
Feature type	transgenic_transposon

Size		Expression data	GFP reporter
Associated insertions	0 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Strutt, 2001)
Uses	CV term Qualifiers & info Reference characterization fz (Strutt, 2001) reporter construct vital \| fz (Strutt, 2001)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference molecular tag (Strutt, 2001)
Component Alleles
Allele	fz^{arm.T:Avic\GFP-EGFP}
Reference(s)	(Yan et al., 2009, Amonlirdviman et al., 2005, Tree et al., 2002, Collier et al., 2005, Bastock et al., 2003, Ma et al., 2003, Lee and Adler, 2004, Bellaiche et al., 2004, Strutt, 2003.10.8, Katanaev et al., 2005, Adler et al., 2004, Strutt, 2001, Strutt and Strutt, 2003, Chung et al., 2007, Wu and Mlodzik, 2008, Chung et al., 2009, Jankovics and Brunner, 2006, Gault et al., 2012)
Molecular data	Construct: The C terminal end of the full-length open reading frame of fz is fused to the N terminal end of T:Avic\GFP^EGFP. This is driven by the arm promoter. (Strutt, 2001)
Phenotypic class	planar polarity defective, with Scer\GAL4^hs.2sev, fz^Scer\UAS.cAa (Chung et al., 2007) wild-type (Strutt, 2001)
Phenotype manifest in	ommatidium, with Scer\GAL4^hs.2sev, fz^Scer\UAS.cAa (Chung et al., 2007)
Other information
Expression Data
Reporter Expression
distribution deduced from reporter or direct label Stage Tissue/Position (including subcellular localization) Reference pupal stage P6 -- P7 cell \| distal of arista (He and Adler, 2002) Comment:reference states 26-40 hr APF cell \| distal of wing (He and Adler, 2002)
Additional Information
Statement Reference fz^{arm.T:Avic\GFP-EGFP} protein is localized preferentially to the distal side of cells comprising the arista during pupal development. (He and Adler, 2002)

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	arm::fz-GFP (Lee and Adler, 2004) arm-Fz::GFP (Bellaiche et al., 2004) Arm-Fz-EGFP (Strutt, 2003.10.8) arm-fz-GFP (Collier et al., 2005, Adler et al., 2004, Chung et al., 2007, Yan et al., 2009, Chung et al., 2009, Wu and Mlodzik, 2008, Gault et al., 2012) arm-Fz-GFP (Katanaev et al., 2005) Arm-Fz-GFP (Strutt, 2003.10.8) Fz::GFP (Amonlirdviman et al., 2005, Ma et al., 2003) Fz-GFP (Strutt and Strutt, 2003, Strutt, 2001, Jankovics and Brunner, 2006) P[w⁺,Arm-Fz-GFP] (Bastock et al., 2003) P{arm-fz.GFP} (FlyBase, 1996-)
Secondary FlyBase IDs

References ( 18 )
Research paper	Gault et al., 2012, J. Cell Biol. 196(5): 605--621 Drosophila CK1-γ, gilgamesh, controls PCP-mediated morphogenesis through regulation of vesicle trafficking. [FBrf0217630] Chung et al., 2009, PLoS Genet. 5(11): e1000746 Serrano (sano) functions with the planar cell polarity genes to control tracheal tube length. [FBrf0209469] Yan et al., 2009, Dev. Biol. 333(1): 186--199 Rho1 has multiple functions in Drosophila wing planar polarity. [FBrf0208582] Wu and Mlodzik, 2008, Dev. Cell 15(3): 462--469 The frizzled extracellular domain is a ligand for Van Gogh/Stbm during nonautonomous planar cell polarity signaling. [FBrf0206051] Chung et al., 2007, Genetics 176(2): 891--903 The balance between the novel protein target of wingless and the Drosophila Rho-associated kinase pathway regulates planar cell polarity in the Drosophila wing. [FBrf0202398] Jankovics and Brunner, 2006, Dev. Cell 11(3): 375--385 Transiently reorganized microtubules are essential for zippering during dorsal closure in Drosophila melanogaster. [FBrf0194305] Amonlirdviman et al., 2005, Science 307(5708): 423--426 Mathematical modeling of planar cell polarity to understand domineering nonautonomy. [FBrf0188460] Collier et al., 2005, Genetics 169(4): 2035--2045 The WD40 repeat protein fritz links cytoskeletal planar polarity to frizzled subcellular localization in the Drosophila epidermis. [FBrf0187636] Katanaev et al., 2005, Cell 120(1): 111--122 Trimeric G protein-dependent frizzled signaling in Drosophila. [FBrf0183772] Adler et al., 2004, Curr. Biol. 14(22): 2046--2051 Inturned localizes to the proximal side of wing cells under the instruction of upstream planar polarity proteins. [FBrf0180074] Bellaiche et al., 2004, Development 131(2): 469--478 The planar cell polarity protein Strabismus promotes Pins anterior localization during asymmetric division of sensory organ precursor cells in Drosophila. [FBrf0167532] Lee and Adler, 2004, Mech. Dev. 121(1): 37--49 The grainy head transcription factor is essential for the function of the frizzled pathway in the Drosophila wing. [FBrf0167844] Bastock et al., 2003, Development 130(13): 3007--3014 Strabismus is asymmetrically localised and binds to Prickle and Dishevelled during Drosophila planar polarity patterning. [FBrf0160398] Ma et al., 2003, Nature 421(6922): 543--547 Fidelity in planar cell polarity signalling. [FBrf0155499] Strutt and Strutt, 2003, Curr. Biol. 13(16): 1451--1457 EGF signaling and ommatidial rotation in the Drosophila eye. [FBrf0162001] Tree et al., 2002, Cell 109(3): 371--381 Prickle mediates feedback amplification to generate asymmetric planar cell polarity signaling. [FBrf0148922] Strutt, 2001, Mol. Cell 7(2): 367--375 Asymmetric localization of frizzled and the establishment of cell polarity in the Drosophila wing. [FBrf0134764]
Personal communication to FlyBase	Strutt, 2003.10.8, Arm-Fz-GFP and Act5C-FRT-Stop-FRT-Fz-EYFP constructs. Arm-Fz-GFP and Act5C-FRT-Stop-FRT-Fz-EYFP constructs. [FBrf0173063]