Recombinant construct P{Gr22e-GAL4.D}

General Information
Symbol	P{Gr22e-GAL4.D}	FlyBase ID	FBtp0014666
Feature type	transgenic_transposon

Size		Expression data	GAL4 reporter/driver
Associated insertions	0 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Dunipace et al., 2001)
Uses	CV term Qualifiers & info Reference characterization Gr22e (Dunipace et al., 2001)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference
Component Alleles
Allele	Scer\GAL4^Gr22e.PD
Reference(s)	(Fishilevich et al., 2005, Wang et al., 2004, Thorne et al., 2004, Dunipace et al., 2001, Bray and Amrein, 2003, Thorne and Amrein, 2008, Hiroi et al., 2002)
Molecular data	Construct: Gr22e promoter sequences drive expression of Scer\GAL4. (Dunipace et al., 2001)
Phenotypic class	mating defective \| male, with Ctet\TeTxLC^TNT.Scer\UAS (Bray and Amrein, 2003) taste perception defective, with Ctet\TeTxLC^TNT.Scer\UAS (Thorne et al., 2004)
Phenotype manifest in
Other information
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS) Stage Tissue/Position (including subcellular localization) Reference adult stage tibia \| restricted (Dunipace et al., 2001) gustatory receptor neuron of tarsal segment \| subset (Dunipace et al., 2001) subesophageal ganglion \| restricted (Dunipace et al., 2001) gustatory receptor neuron of tibia \| subset (Dunipace et al., 2001) labellar taste bristle \| subset (Dunipace et al., 2001) third segment of antenna \| restricted (Dunipace et al., 2001) gustatory receptor neuron \| subset (Dunipace et al., 2001, Bray and Amrein, 2003, Miyamoto and Amrein, 2008) wing margin bristle \| subset (Dunipace et al., 2001) gustatory receptor neuron of wing margin bristle \| subset (Dunipace et al., 2001) olfactory receptor neuron of maxillary palpus \| subset (Dunipace et al., 2001) olfactory receptor neuron of third segment of antenna \| subset (Dunipace et al., 2001) ventral cibarial sense organ neuron of proximal sensillum \| subset (Dunipace et al., 2001) dorsal cibarial sense organ neuron \| subset (Dunipace et al., 2001) maxillary palpus \| restricted (Dunipace et al., 2001) gustatory receptor neuron of labellar taste bristle \| subset (Dunipace et al., 2001, Thorne and Amrein, 2008) tarsal segment \| restricted (Dunipace et al., 2001) olfactory receptor neuron \| subset (Dunipace et al., 2001) labral sense organ neuron \| subset (Dunipace et al., 2001) chemosensory neuron of labellum (Thorne et al., 2004) chemosensory neuron of adult labral sense organ (Thorne et al., 2004) chemosensory neuron of ventral cibarial sense organ (Thorne et al., 2004) chemosensory neuron of mesothoracic leg (Thorne et al., 2004) chemosensory neuron of metathoracic leg (Thorne et al., 2004) chemosensory neuron of prothoracic leg (Thorne et al., 2004) gustatory receptor neuron of proboscis (Wang et al., 2004) gustatory receptor neuron of labellum (Wang et al., 2004) I-type sensilla I9 \| faint (Hiroi et al., 2002) I-type sensilla I10 \| faint (Hiroi et al., 2002) S-type sensilla S1 (Hiroi et al., 2002) S-type sensilla S2 (Hiroi et al., 2002) S-type sensilla S3 (Hiroi et al., 2002) S-type sensilla S4 \| faint (Hiroi et al., 2002) S-type sensilla S5 (Hiroi et al., 2002) S-type sensilla S6 (Hiroi et al., 2002) S-type sensilla S7 (Hiroi et al., 2002) S-type sensilla S8 \| faint (Hiroi et al., 2002) S-type sensilla S9 (Hiroi et al., 2002) S-type sensilla S10 (Hiroi et al., 2002) S-type sensilla S11 (Hiroi et al., 2002) S-type sensilla S12 (Hiroi et al., 2002) gustatory receptor neuron of short labellar taste bristle \| subset (Thorne and Amrein, 2008) gustatory receptor neuron of intermediate labellar taste bristle \| subset (Thorne and Amrein, 2008) prothoracic leg taste bristle chemosensory neuron \| subset (Miyamoto and Amrein, 2008)
Additional Information
Statement Reference Adult expression is observed in about 60 gustatory sensilla, including and average of 4 in the prothoracic leg, 8.2 in the mesothoracic leg, 5.7 in the metathoracic leg, 9.8 in the wing margin, 15 in the labial palps and in the labral and cibarial sense organs. (Bray and Amrein, 2003) Expression of Scer\GAL4[Gr22e.PD] is found in large and small neurons associated with the S-type bristles of the labellum. (Thorne and Amrein, 2008) Scer\GAL4[Gr22e.PD] is expressed in approximately 14 cells per labial palp, 2 cells per first leg, 8 cells per second leg and 10 cells per third leg. (Thorne et al., 2004) Adult expression is observed in about 60 gustatory sensilla, including cells at the base of chemosensory bristles in the labial palps, in cells in the dorsal and ventral cibarial sense organs, along the tibiae and tarsi of the legs, in cells at the base of chemosensory bristles along the anterior wing margin, and in cells in third antennal segment and in the maxillary palp. Axons of Gr22e-expressing neurons project to the subesophageal gangion. (Dunipace et al., 2001)

Marker for	gustatory neuron \| subset (Dunipace et al., 2001, Bray and Amrein, 2003)
Reflects expression of	Gr22e (Dunipace et al., 2001, Bray and Amrein, 2003)
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	[22e]->Gal4 (Dunipace et al., 2001) Gr22e (Hiroi et al., 2002) Gr22e-GAL4 (Wang et al., 2004, Thorne and Amrein, 2008) p[Gr22e]-Gal4 (Thorne et al., 2004, Bray and Amrein, 2003) P{Gr22e-GAL4.D} (FlyBase, 1996-)
Secondary FlyBase IDs

References ( 7 )
Research paper	Thorne and Amrein, 2008, J. Comp. Neurol. 506(4): 548--568 Atypical expression of Drosophila gustatory receptor genes in sensory and central neurons. [FBrf0201488] Fishilevich et al., 2005, Curr. Biol. 15(23): 2086--2096 Chemotaxis behavior mediated by single larval olfactory neurons in Drosophila. [FBrf0190010] Thorne et al., 2004, Curr. Biol. 14(12): 1065--1079 Taste perception and coding in Drosophila. [FBrf0179479] Wang et al., 2004, Cell 117(7): 981--991 Taste representations in the Drosophila brain. [FBrf0179508] Bray and Amrein, 2003, Neuron 39(6): 1019--1029 A putative Drosophila pheromone receptor expressed in male-specific taste neurons is required for efficient courtship. [FBrf0162264] Hiroi et al., 2002, Zool. Sci., Tokyo 19(9): 1009--1018 Differentiated Response to Sugars among Labellar Chemosensilla in Drosophila. [FBrf0152351] Dunipace et al., 2001, Curr. Biol. 11(11): 822--835 Spatially restricted expression of candidate taste receptors in the Drosophila gustatory system. [FBrf0137203]