Recombinant construct P{Gr28b.e-GAL4.4.245}

General Information
Symbol	P{Gr28b.e-GAL4.4.245}	FlyBase ID	FBtp0014672
Feature type	transgenic_transposon

Size		Expression data	GAL4 reporter/driver
Associated insertions	0 available
Molecular map
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Scott et al., 2001)
Uses	CV term Qualifiers & info Reference characterization Gr28b (Scott et al., 2001)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference
Component Alleles
Allele	Scer\GAL4^{Gr28b.e.4.245}
Reference(s)	(Scott et al., 2001, Thorne et al., 2004, Colomb and Stocker, 2007, Thorne and Amrein, 2008, Weiss et al., 2011, Weiss et al., 2011, Kwon et al., 2011)
Molecular data	Construct: 4.245kb of Gr28b promoter sequences are fused upstream of Scer\GAL4. (Scott et al., 2001)
Phenotypic class
Phenotype manifest in
Other information
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS) Stage Tissue/Position (including subcellular localization) Reference larval stage terminal organ (Scott et al., 2001) embryonic/larval gut \| restricted (Scott et al., 2001) embryonic/larval mouth \| restricted (Scott et al., 2001) terminal organ \| restricted (Thorne and Amrein, 2008) third instar larval stage gustatory receptor neuron of terminal organ (Kwon et al., 2011) embryonic/larval subesophageal ganglion (Kwon et al., 2011) adult stage chemosensory neuron of labellum (Thorne et al., 2004) chemosensory neuron of ventral cibarial sense organ (Thorne et al., 2004) chemosensory neuron of prothoracic leg (Thorne et al., 2004) labellum (Scott et al., 2001) cibarial sense organ (Scott et al., 2001) gustatory receptor neuron \| subset (Thorne and Amrein, 2008) gustatory receptor neuron of labellar taste bristle \| subset (Thorne and Amrein, 2008) gustatory receptor neuron \| subset of ventral cibarial sense organ (Thorne and Amrein, 2008) gustatory receptor neuron \| faint & subset of prothoracic tarsal segment 5 (Thorne and Amrein, 2008) subesophageal ganglion \| restricted (Thorne and Amrein, 2008) gustatory receptor neuron \| subset of short labellar taste bristle \| subset (Weiss et al., 2011) gustatory receptor neuron \| subset of intermediate labellar taste bristle \| subset (Weiss et al., 2011) L2 GRN of labellum sensillum (Weiss et al., 2011) S-type sensilla S0 (Weiss et al., 2011) Comment:isoform Gr28b-RE S-type sensilla S1 (Weiss et al., 2011) Comment:isoform Gr28b-RE S-type sensilla S3 (Weiss et al., 2011) Comment:isoform Gr28b-RE S-type sensilla S4 (Weiss et al., 2011, Weiss et al., 2011) Comment:isoform Gr28b-RE S-type sensilla S5 (Weiss et al., 2011, Weiss et al., 2011) Comment:isoform Gr28b-RE S-type sensilla S6 (Weiss et al., 2011) Comment:isoform Gr28b-RE S-type sensilla S7 (Weiss et al., 2011) Comment:isoform Gr28b-RE S-type sensilla S10 (Weiss et al., 2011, Weiss et al., 2011) Comment:isoform Gr28b-RE S-type sensilla S11 (Weiss et al., 2011) Comment:isoform Gr28b-RE I-type sensilla I8 (Weiss et al., 2011, Weiss et al., 2011) Comment:isoform Gr28b-RE I-type sensilla I9 (Weiss et al., 2011) Comment:isoform Gr28b-RE I-type sensilla I10 (Weiss et al., 2011, Weiss et al., 2011) Comment:isoform Gr28b-RE S-type sensilla S12 (Weiss et al., 2011) Comment:isoform Gr28b-RE S-type sensilla S0 \| intense (Weiss et al., 2011) Comment:isoform Gr28b-RE S-type sensilla S1 \| intense (Weiss et al., 2011) Comment:isoform Gr28b-RE S-type sensilla S3 \| intense (Weiss et al., 2011) Comment:isoform Gr28b-RE S-type sensilla S6 \| intense (Weiss et al., 2011) Comment:isoform Gr28b-RE S-type sensilla S7 \| intense (Weiss et al., 2011) Comment:isoform Gr28b-RE S-type sensilla S11 \| intense (Weiss et al., 2011) Comment:isoform Gr28b-RE I-type sensilla I9 \| intense (Weiss et al., 2011) Comment:isoform Gr28b-RE S-type sensilla S12 \| intense (Weiss et al., 2011) Comment:isoform Gr28b-RE
Additional Information
Statement Reference Scer\GAL4[Gr28b.e.4.245] is expressed in gustatory neurons of all of the bitter-sensitive small labellar taste bristles, and in gustatory neurons of the four posterior-most intermediate labellar taste bristles. (Weiss et al., 2011) Expression of Scer\GAL4[Gr28b.e.4.245] is seen in approximately 13 neurons per labial palp. Scer\GAL4[Gr28b.e.4.245] is found to be expressed in a single neuron associated with the ventral cibarial sense organ. Expression of Scer\GAL4[Gr28b.e.4.245] is found in two neurons associated with the fifth tarsal segment of the adult foreleg. (Thorne and Amrein, 2008) Scer\GAL4[Gr28b.e.4.245] expresses the isoform Gr28b-RE which is found in all of the short and in a few of the intermediate bitter-sensitive sensilla of the labellum. (Weiss et al., 2011) Scer\GAL4[Gr28b.e.4.245] is expressed in all of the short and in a few of the intermediate bitter-sensitive sensilla of the labellum. (Weiss et al., 2011) Scer\GAL4[Gr28b.e.4.245] expresses in 8-10 neurons innervating small or medium bristles on the labellum. (Thorne et al., 2004) Scer\GAL4[Gr28b.e.4.245] drives expression in one gustatory receptor neuron of the larval terminal organ. This neuron also expresses Scer\GAL4[Gr33a.4.7], Scer\GAL4[Gr66a.1.8], Scer\GAL4[Gr32a.3.7.PW], Scer\GAL4[Gr39a.a.3.4], Scer\GAL4[Gr39a.b.1], Scer\GAL4[Gr59d.4.1], Scer\GAL4[Gr9a.6.3], Scer\GAL4[Gr22e.2.5], Scer\GAL4[Gr22a.4.2], Scer\GAL4[Gr28b.a.3], Scer\GAL4[Gr36b.0.9], Scer\GAL4[Gr36c.2.5], Scer\GAL4[Gr58b.1], Scer\GAL4[Gr59a.1.5], Scer\GAL4[Gr59c.5.5] and Scer\GAL4[Gr93b.5.3]. (Kwon et al., 2011)

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	Gr28b.e-Gal4 (Thorne and Amrein, 2008) Gr28b.e-GAL4 (Weiss et al., 2011, Kwon et al., 2011) Gr28be-Gal4 (Colomb and Stocker, 2007) p[Gr28be]-Gal4 (Thorne et al., 2004) P{Gr28b.e-GAL4.4.245} (FlyBase, 1996-) P{GRLU.2-GAL4.4.245} (FlyBase, 1996-)
Secondary FlyBase IDs

References ( 7 )
Research paper	Kwon et al., 2011, J. Neurosci. 31(43): 15300--15309 Molecular and cellular organization of the taste system in the Drosophila larva. [FBrf0216529] Weiss et al., 2011, Neuron 69(2): 258--272 The Molecular and Cellular Basis of Bitter Taste in Drosophila. [FBrf0212834] Thorne and Amrein, 2008, J. Comp. Neurol. 506(4): 548--568 Atypical expression of Drosophila gustatory receptor genes in sensory and central neurons. [FBrf0201488] Colomb and Stocker, 2007, Fly 1(4): 232--234 Combined rather than separate pathways for hedonic and sensory aspects of taste in fly larvae? [FBrf0204238] Thorne et al., 2004, Curr. Biol. 14(12): 1065--1079 Taste perception and coding in Drosophila. [FBrf0179479] Scott et al., 2001, Cell 104(5): 661--673 A chemosensory gene family encoding candidate gustatory and olfactory receptors in Drosophila. [FBrf0134489]
Supplementary material	Weiss et al., 2011, Neuron 69(2): Supplemental Information. [FBrf0213039]