A Database of Drosophila Genes & Genomes

FB2008_07, released August 8, 2008
 

Recombinant construct P{Cha-GAL4.7.4}

General Information
Symbol P{Cha-GAL4.7.4} FlyBase ID FBtp0014830
Feature type transgenic_transposon Created / Updated 2001-10-14/2003-07-07
Size Expression data GAL4 reporter/driver
Location-dependent role Associated insertions 1 available
Molecular map
hide Description & Uses
Species synthetic construct
Description
CV term
Qualifiers & info
Reference
transposon
P-element
(Wittmann et al., 2001, Lima and Miesenbock, 2005)
Uses
CV term
Qualifiers & info
Reference
characterization
Cha
(Wittmann et al., 2001)
binary system (regulatory)
shakB
(Lima and Miesenbock, 2005)
Cloning Sites
Location
Restriction sites
Reference
hide Sequence Data
Sequence (FB)
Sequence
Associated Sequence Data
DDBJ /
EMBL /
GenBank
DNA sequence
Extent
 
hide Segments & Size
Total Size
Left end
Right end
Segments
Number
Orientation
Symbol
Reference
hide Features
CV term
Qualifiers & info
Reference
binary
component
(Lima and Miesenbock, 2005)
hide Component Alleles
hideScer\GAL4Cha.7.4
Reference(s) (Banerjee et al., 2004, Lima and Miesenbock, 2005, Nassif et al., 2003, Younossi-Hartenstein et al., 2003, Dumstrei et al., 2003, Sanyal, 2005, Keegan et al., 2005, Rajaram et al., 2005, Walker et al., 2006, Su and O'Dowd, 2003, Baines et al., 2002, Salvaterra and Kitamoto, 2001, Lee and Salvaterra, 2002, Joshi et al., 2004, Nishimura et al., 2004, Hekmat-Scafe et al., 2005, Wittmann et al., 2001, Luan et al., 2006, Garg et al., 2007, Meyer and Aberle, 2006)
Molecular data
Construct: Cha sequences drive expression of Scer\GAL4.
(Wittmann et al., 2001)
Construct: A 7.4kb BamHI 5' flanking fragment from the "cholinergic" locus which encodes both Cha and VAChT function) drives expression of Scer\GAL4.
(Salvaterra and Kitamoto, 2001)
Phenotypic class
neuroanatomy defective, with Hsap\MAPTR406W.Scer\UAS
(Nishimura et al., 2004)
viable, with Adar3.4.S.Scer\UAS, Adar5G1
(Keegan et al., 2005)
lethal | larval stage, with acj61,4.Scer\UAS
(Lee and Salvaterra, 2002)
lethal | pupal stage, with acj61,4.Scer\UAS
(Lee and Salvaterra, 2002)
neurophysiology defective | conditional - heat sensitive, with shi1.Scer\UAS
(Rajaram et al., 2005)
short lived, with Rnor\P2rx2Scer\UAS.cLa
(Lima and Miesenbock, 2005)
neurophysiology defective | larval stage | drug conditional, with Rnor\P2rx2Scer\UAS.cLa
(Lima and Miesenbock, 2005)
neurophysiology defective | larval stage, with Rnor\P2rx2Scer\UAS.cLa
(Lima and Miesenbock, 2005)
locomotor behavior defective | adult stage | drug conditional, with Rnor\P2rx2Scer\UAS.cLa
(Lima and Miesenbock, 2005)
paralytic | adult stage | drug conditional, with Rnor\P2rx2Scer\UAS.cLa
(Lima and Miesenbock, 2005)
Phenotype manifest in
lamina, with Hsap\MAPTR406W.Scer\UAS
(Wittmann et al., 2001)
central body complex, with Hsap\MAPTR406W.Scer\UAS
(Wittmann et al., 2001)
neuron, with Hsap\MAPTR406W.Scer\UAS
(Nishimura et al., 2004)
lamina, with Hsap\MAPTScer\UAS.cWa
(Wittmann et al., 2001)
Other information
hidew+mC
Reference(s) (Salvaterra and Kitamoto, 2001)
Molecular data
Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
 
4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site.
(Pirrotta, 1988)
Minigene of the white cDNA.
(Chung et al., 1993)
Deletion of w+mC 5' flanking sequences to position -305.
(Qian and Pirrotta, 1995)
Phenotypic class
courtship defective | male
(Lloyd et al., 2002, Hing and Carlson, 1996)
visible | dominant
(True et al., 1996)
visible | recessive
(Roseman et al., 1993)
eye color defective
(Soldatov et al., 1999, Hagstrom et al., 1996)
eye color defective, with Scer\GAL4Act5C.PI, wSym.Scer\UAS, wa.Sym.Scer\UAS
(Giordano et al., 2002)
Phenotype manifest in
pigment cell
(Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995)
pigment cell, with Scer\GAL4Act5C.PI, wSym.Scer\UAS, wa.Sym.Scer\UAS
(Giordano et al., 2002)
Other information
hide Expression Data
Reporter Expression
Stage
Tissue/Position
Reference
  adult stage
  neuron | subset
(Wittmann et al., 2001)
  adult stage
  cholinergic neuron
(Wittmann et al., 2001)
Additional Information
Statement
Reference
Assay mode
distribution deduced from reporter (Gal4 UAS)
(Wittmann et al., 2001)
distribution deduced from reporter (Gal4 UAS)
(Wittmann et al., 2001)
Marker for
cholinergic neuron
(Wittmann et al., 2001)
Reflects expression of
Cha
(Wittmann et al., 2001)
hide Progenitors & Descendants
progenitor(s)
descendant(s)
hide Comments
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
(ChAT)-GAL4
(Dumstrei et al., 2003)
Cha-GAL4
(Keegan et al., 2005, Hekmat-Scafe et al., 2005, Nishimura et al., 2004, Baines et al., 2002, Wittmann et al., 2001, Walker et al., 2006)
chaGAL4
(Joshi et al., 2004)
Cha-Gal4
(Su and O'Dowd, 2003, Lee and Salvaterra, 2002, Luan et al., 2006, Meyer and Aberle, 2006)
ChaGal4
(Garg et al., 2007)
Cha-Gal4-19B
(Rajaram et al., 2005)
ChAT-GAL4
(Younossi-Hartenstein et al., 2003)
ChATGAL4
(Banerjee et al., 2004)
P{Cha-GAL4.7.4}
(FlyBase, 1996-, Lima and Miesenbock, 2005)
P{Cha-GAL4.W}
(FlyBase, 1996-)
pCaSpeR2-Cha-Gal4
(Salvaterra and Kitamoto, 2001)
Secondary FlyBase IDs
    hide References ( 32 )
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    hide Recent research papers ( 4 )
    Garg et al., 2007, Genetics 175(2): 659--669
    Antagonizing scalloped with a novel vestigial construct reveals an important role for scalloped in Drosophila melanogaster leg, eye and optic lobe development. [FBrf0193374]
    Luan et al., 2006, Neuron 52(3): 425--436
    Refined spatial manipulation of neuronal function by combinatorial restriction of transgene expression. [FBrf0193617]
    Meyer and Aberle, 2006, Development 133(20): 4035--4044
    At the next stop sign turn right: the metalloprotease Tolloid-related 1 controls defasciculation of motor axons in Drosophila. [FBrf0193012]
    Walker et al., 2006, Genes Dev. 20(23): 3311--3323
    Reduced growth of Drosophila neurofibromatosis 1 mutants reflects a non-cell-autonomous requirement for GTPase-Activating Protein activity in larval neurons. [FBrf0193208]
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