FlyBase Recombinant Construct Report: P{ChAT-GAL4.7.4}

ScerGAL4^ChAT.7.4 and ScerGAL4^S3.Hug drive expression in larval Hugin neuron of the protocerebrum, larval Hugin neuron of the ventral nerve cord, larval Hugin neuron of the ventral nerve cord as well as, weakly, larval Hugin neuron of the corpus cardiacum.

(Schlegel et al., 2016)

ScerGAL4^ChAT.7.4 colocalizes with eve in EL neurons.

(Heckscher et al., 2015)

ScerGAL4^Cha.7.4 labels a subset of neurons in the lamina, medulla, lobula and lobula plate. The labelled cells include: Tm1, Tm2, Tm4, Tm4a, Tm7, Tm8, Tm11, Tm12, Tm15, Tm22, Tm24, TmY2, TmY5, TmY9, TmY10, TmY11, TmY12, Mi2, Mi7, Mi11, Pm1, Pm1a, Mt4, Mt10, T2, T4, Tlp2, Tlp3, Lcn1, Lcn2, Lt6, Tm new 1, Tm new 2, Tm new 3, T new 1, mY new 1, Mt new 1, Mi new 1, Mi new 2, Mi new 3, Lcn new 1, Loi new 1 and lpi new 1.

(Varija Raghu et al., 2011)

ScerGAL4^Cha.7.4 drives expression in the Rh5 and Rh6 photoreceptors of Bolwig organ in the larval optic neuropil.

(Keene et al., 2011)

Expression in the midgut is found in a distinct group of Dh31-expressing enteroendocrine cells located at the junction region of the anterior midgut and the acid-secreting portion of the larval midgut. This junction region occurs at a conspicuous and characteristic U-shaped bend in the gut. These are referred to as the "midgut junction Dh31-expressing cells". There is no overlap in expression between this marker and ChAT expression in the midgut.

(Lajeunesse et al., 2010)

ScerGAL4^Cha.7.4 labels at least four LNd neurons and the Pdf negative s-LNv neuron. The four LNd neurons are also labeled by expression of per protein. Two of these four LNd neurons also co-express sNPF protein.

(Johard et al., 2009)

Most of the ScerGAL4^Cha.7.4 expressing neurons do not colocalize with sNPF protein in the adult. However, there is colocalization in a subset of columnar neurons at the ventral base of the optic lobe with dense termination sites in the lateral protocerebrum.

(Nässel et al., 2008)

Expression of ScerGAL4^Cha.7.4 is limited to the ventral part of the ventral nerve cord.

(Daniels et al., 2008)

In adults, ScerGAL4^VGlut-OK371 drives expression in thin neurites that innervate the beta gamma lobes, especially in the anterior region of the latter, and in the neuropils surrounding the lobes and peduncle. Co-localisation with sNPF protein is observed in a small number of cell bodies throughout the brain and in the neuronal axon tract anterior to the beta and gamma lobes. Larval expression was similar to the one in adults, with additional expression in processes that terminate in the calyx or that surround the dorsal tip of the vertical lobe.

(Johard et al., 2008)

ScerGAL4^ChAT.7.4 drives expression in cholinergic neurons from the onset of synaptogenesis.

(Baines et al., 2002)

Marker for

adult optic lobe

(Nässel et al., 2008)

cholinergic neuron

(Schlegel et al., 2016, Wittmann et al., 2001)

Reflects expression of

ChAT

(Schlegel et al., 2016, Heckscher et al., 2015, Chin et al., 2014, Chen et al., 2012, Keene et al., 2011, Varija Raghu et al., 2011, Boerner and Duch, 2010, Wu et al., 2010, Johard et al., 2009, Hamada et al., 2008, Johard et al., 2008, Kolodziejczyk et al., 2008, Zhou et al., 2008, Beramendi et al., 2007, Wittmann et al., 2001)

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

descendant(s)

Comments

Stocks (3)

Stocks containing insertion(s) of this recombinant construct

Bloomington

6793

w^*; P{ChAT-GAL4.7.4}19B P{UAS-GFP.S65T}Myo31DF^T2

56501

w^*; P{ET-GAL80}Gaz, P{ChAT-GAL4.7.4}19B P{UAS-GFP.S65T}Myo31DF^T2/CyO

56500

w^*; P{ChAT-GAL4.7.4}19B/CyO; PBac{20XUAS-IVS-GCaMP5G}VK00005/TM3, Sb¹

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (62)

Reported As

Symbol Synonym

(ChAT)-GAL4

(Dumstrei et al., 2003)

7.4kb Cha-Gal4

(Ling et al., 2014)

B19-GAL4

(Baines, 2004, Baines et al., 2002)

B19-Gal4

(Fradkin et al., 2008)

CHA-Gal4

(Cukier et al., 2008, Kaplan et al., 2008)

CHAGal4

(Caygill and Johnston, 2008)

ChA-GAL4

(Grabe et al., 2016, Melnattur et al., 2013)

ChAT GAL4

(Søvik et al., 2017)

ChAT-7.4

(Sonn et al., 2018)

ChAT-GAL4

(Giordani et al., 2023, Byers et al., 2021, Douthit et al., 2021, Delventhal and Steinhauer, 2020, Chen et al., 2019, Hill et al., 2019, Hsieh et al., 2019, Dean et al., 2018, Farley et al., 2018, Fedotov et al., 2018, Jayakumar et al., 2016, Schlegel et al., 2016, Younossi-Hartenstein et al., 2003)

ChAT-Gal4

(Bazzi et al., 2023, Chen et al., 2023, Rey et al., 2023, Ábrahám et al., 2022, Mattedi et al., 2022, Li et al., 2021, Lund et al., 2021, Deng et al., 2020, Rallis et al., 2020, Weaver et al., 2020, Held et al., 2019, Qin et al., 2019, Lee et al., 2018, Boppana et al., 2017, Tuthill and Wilson, 2016, Cardona et al., 2009, Pereanu and Hartenstein, 2006)

ChAT-Gal4.7.4

(Mohammad et al., 2017)

ChAT-Gal4.7.4-19B

(Wang et al., 2020)

ChAT-gal4

(Chng et al., 2017)

ChAT-gal4 7.4

(Heckscher et al., 2015)

ChAT^7.4-GAL4

(Delventhal and Steinhauer, 2020)

ChATGAL4

(Hamid et al., 2021, Hamid et al., 2019, Banerjee et al., 2004)

Cha GAL4

(Kahsai and Winther, 2011)

Cha Gal

(Lajeunesse et al., 2010)

Cha(7.4)-GAL4

(Hamada et al., 2008)

Cha(7.4)-Gal4

(Yao et al., 2012)

Cha-GAL4

(Lyu et al., 2023, Bolshakova et al., 2022, Yalgin et al., 2020, Castells-Nobau et al., 2019, Ryazantseva et al., 2018, Laneve et al., 2017, Saras et al., 2017, Noble et al., 2016, Yilmazer et al., 2016, Bai and Sehgal, 2015, Cassar et al., 2015, Shih et al., 2015, Vanderwerf et al., 2015, Chin et al., 2014, Iyengar, 2014.6.6, Murali et al., 2014, Min et al., 2013, Thran et al., 2013, Timmerman et al., 2013, Nam et al., 2012, Sadananda et al., 2012, Tayler et al., 2012, Timmerman and Sanyal, 2012, Zhou et al., 2012, Chiang et al., 2011, Kahsai and Winther, 2011, Keegan et al., 2011, Schnakenberg et al., 2011, Varija Raghu et al., 2011, Boerner and Duch, 2010, Hekmat-Scafe et al., 2010, Repnikova et al., 2010, Dankert et al., 2009, Iijima-Ando et al., 2009, Lee et al., 2009, Beramendi et al., 2007, Mehren and Griffith, 2006, Walker et al., 2006, Hekmat-Scafe et al., 2005, Keegan et al., 2005, Ghosh and Feany, 2004, Nishimura et al., 2004, Baines et al., 2002, Kitamoto, 2001, Wittmann et al., 2001)

Cha-GAL4-19B

(Sakai et al., 2009)

Cha-Gal4

(Tsumaki et al., 2024, Aimon et al., 2023, Das Chakraborty et al., 2022, Schmidt et al., 2022, Georganta et al., 2021, Lund et al., 2021, Tian, 2021, Bataillé et al., 2020, Davla et al., 2020, Pirooznia et al., 2020, White et al., 2020, Fenckova et al., 2019, Smith et al., 2019, Bai et al., 2018, Sonn et al., 2018, Mohammad et al., 2017, Yoon et al., 2017, Garelli et al., 2015, Jeong et al., 2015, Okamoto and Nishimura, 2015, Schinaman et al., 2014, Schoofs et al., 2014, Yuan et al., 2014, Buchon et al., 2013, Ghezzi et al., 2013, Lin et al., 2013, Wiemerslage et al., 2013, Chen et al., 2012, Humphrey et al., 2012, Imlach et al., 2012, Iyengar, 2012, Lai et al., 2012, Lotti et al., 2012, Pfeiffenberger and Allada, 2012, Rezaval et al., 2012, Chiang et al., 2011, Iyengar et al., 2011, Kahsai and Winther, 2011, Keene et al., 2011, Shuai et al., 2011, Stavropoulos and Young, 2011, Carlsson et al., 2010, Choi et al., 2010, Lajeunesse et al., 2010, Melicharek et al., 2010, Wu et al., 2010, Johard et al., 2009, Ling et al., 2009, Johard et al., 2008, Kolodziejczyk et al., 2008, Nässel et al., 2008, Pauli et al., 2008, Zhou et al., 2008, Luan et al., 2006, Meyer and Aberle, 2006, Su and O'Dowd, 2003, Lee and Salvaterra, 2002)

Cha-Gal4(19B)

(Lin et al., 2017)

Cha-Gal4-19B

(Kanellopoulos et al., 2020, Rajaram et al., 2005)

Cha-Gal80)

(Certel et al., 2010)

Cha-gal4

(Iijima-Ando et al., 2009, Wegener et al., 2004)

Cha7.4-Gal4

(Sen et al., 2014)

Cha^7.4-GAL4

(Kondo et al., 2020, Mauss et al., 2009)

Cha^7.4-Gal4

(Diao et al., 2015)

Cha^B19-GAL4

(Cash et al., 2016, Mauss et al., 2009)

ChaGal4

(Goodchild et al., 2024, Lajeunesse et al., 2010, Garg et al., 2007, Baqri et al., 2006)

ChaT-GAL4

(Hürkey et al., 2023, Nandakumar et al., 2020, Yoshinari et al., 2020)

Chat-GAL4

(Hussain et al., 2024, Horne et al., 2017, Lacin et al., 2009)

Chat-Gal4

(Zhang et al., 2022, Showell et al., 2020, Slater et al., 2015, Levy and Larsen, 2013, de Velasco et al., 2007)

Choline acetyltransferase (Cha)-Gal4

(Choi et al., 2010)

GAL4-Cha

(Gavin et al., 2006)

Gal4^Chat19b

(Werner et al., 2024)

P(Cha-GAL4.7.4)

(Johard et al., 2009)

P{^w+mC= Cha-GAL4.7.4}

(Schnakenberg et al., 2011)

P{ChAT-GAL4.7.4}

(Hürkey et al., 2023, Golomidov et al., 2020, Held et al., 2019, Iyengar, 2016.3.5, Vanderwerf et al., 2015, FlyBase, 1996-)

P{ChAT-Gal4}

(Iyengar et al., 2006)

P{Cha-GAL4.7.4}

(Martín-Peña et al., 2014, Humphrey et al., 2012, Lima and Miesenbock, 2005, FlyBase, 1996-)

P{Cha-GAL4.W}

(FlyBase, 1996-)

cha B19-GAL4

(Baines et al., 2002)

cha-GAL4

(Peng et al., 2019, Barwell et al., 2017, Liu et al., 2017, Liu et al., 2016, Kunst et al., 2014, Sluder et al., 2012, Boerner and Duch, 2010, Wu et al., 2009, Suster et al., 2004)

cha-Gal4

(Lowe et al., 2024, Seidner et al., 2015, Shiraishi et al., 2014, Inada et al., 2011, Jepson et al., 2011, Daniels et al., 2008, Gao et al., 2008)

cha-gal4

(Schmid et al., 2006)

cha-gal4.7.4

(Wu et al., 2008, Wu et al., 2008)

cha19bGal4

(Jana et al., 2018)

cha7.4

(Berni et al., 2012)

cha^B19

(Vernon et al., 2019)

cha^B19-GAL4

(Vernon et al., 2018)

chaGAL4

(McIlroy et al., 2013, Joshi et al., 2004)

chaGal4

(Dey and Ray, 2018, Rai et al., 2018, Stork et al., 2008)

chat-GAL4

(Sang et al., 2007)

choline acetyltransferase (Cha)-Gal4

(Kim et al., 2011)

choline acetyltransferase (cha) promoter-GAL4

(Suster et al., 2004)

choline acetyltransferase GAL4

(Kahsai and Winther, 2011)

choline acetyltransferase-Gal4

(Humphrey et al., 2012)

pCaSpeR2-Cha-Gal4

(Salvaterra and Kitamoto, 2001)

Secondary FlyBase IDs

References (217)

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