Recombinant construct P{UAS-Hsap\MAPT.R406W}

General Information
Symbol	P{UAS-Hsap\MAPT.R406W}	FlyBase ID	FBtp0014840
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	2 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02	Controlled Vocabulary Terms adult mushroom body day 60 of adulthood
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Wittmann et al., 2001)
Uses	CV term Qualifiers & info Reference binary system (targeted) Hsap\MAPT (Wittmann et al., 2001) characterization Hsap\MAPT (Wittmann et al., 2001)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference binary component (Wittmann et al., 2001)
Component Alleles
Allele	Hsap\MAPT^{R406W.Scer\UAS}
Reference(s)	(Wittmann et al., 2001, Bilen et al., 2006, Nishimura et al., 2004, Grammenoudi et al., 2006, Wang et al., 2007, Ryoo et al., 2007, Mallik and Lakhotia, 2009, Khurana et al., 2006, Fulga et al., 2007, Loewen and Feany, 2010, Malzer et al., 2010, Khurana et al., 2010, Yeh et al., 2010, Duboff et al., 2012, Mershin et al., 2004, Kosmidis et al., 2010, Papanikolopoulou et al., 2010, Bilen and Bonini, 2007)
Molecular data	Construct: Amino acid replacement: R406W. Scer\UAS regulatory sequences drive expression of a mutant form of Hsap\MAPT. (Wittmann et al., 2001)
Phenotypic class	cell cycle defective, with Scer\GAL4^elav.PU (Duboff et al., 2012) cell cycle defective, with Scer\GAL4^elav-C155 (Loewen and Feany, 2010) cell death defective, with Scer\GAL4^elav.PU (Duboff et al., 2012) decreased cell number \| adult stage, with Scer\GAL4^GMR.PF (Wang et al., 2007) increased cell death, with Scer\GAL4^elav.PLu (Fulga et al., 2007) increased cell death, with Scer\GAL4^elav-C155 (Loewen and Feany, 2010, Khurana et al., 2010) increased cell death, with Scer\GAL4^GMR.PF (Bilen et al., 2006) increased cell death \| conditional, with Scer\GAL4^elav.PLu (Khurana et al., 2006) neuroanatomy defective, with Scer\GAL4^Cha.7.4 (Nishimura et al., 2004) neuroanatomy defective, with Scer\GAL4^elav.PU (Duboff et al., 2012, Kosmidis et al., 2010, Papanikolopoulou et al., 2010) neuroanatomy defective, with Scer\GAL4^elav-C155 (Loewen and Feany, 2010) neuroanatomy defective, with Scer\GAL4^hs.2sev (Nishimura et al., 2004) neuroanatomy defective \| day 60 of adulthood, with Scer\GAL4^c492b (Mershin et al., 2004) short lived, with Scer\GAL4^elav-C155 (Khurana et al., 2010) visible, with Scer\GAL4^GMR.PU (Bilen and Bonini, 2007) visible, with Scer\GAL4^hs.2sev (Nishimura et al., 2004) visible \| adult stage, with Scer\GAL4^GMR.PF (Wang et al., 2007)
Phenotype manifest in	adult brain, with Scer\GAL4^elav.PLu (Wittmann et al., 2001) adult brain, with Scer\GAL4^elav.PU (Duboff et al., 2012) adult brain \| conditional, with Scer\GAL4^elav.PLu (Khurana et al., 2006) adult central complex, with Scer\GAL4^Cha.7.4 (Wittmann et al., 2001) adult mushroom body, with Scer\GAL4^elav.PU (Kosmidis et al., 2010, Papanikolopoulou et al., 2010) adult mushroom body \| day 60 of adulthood, with Scer\GAL4^c492b (Mershin et al., 2004) eye, with Scer\GAL4^GMR.PF (Ryoo et al., 2007, Bilen et al., 2006, Mallik and Lakhotia, 2009, Yeh et al., 2010, Wang et al., 2007) eye, with Scer\GAL4^GMR.PU (Bilen and Bonini, 2007) eye, with Scer\GAL4^hs.2sev (Nishimura et al., 2004) eye photoreceptor cell & neuron, with Scer\GAL4^hs.2sev (Nishimura et al., 2004) lamina, with Scer\GAL4^Cha.7.4 (Wittmann et al., 2001) lamina \| conditional, with Scer\GAL4^elav.PLu (Khurana et al., 2006) macrochaeta, with Scer\GAL4^Eq1 (Yeh et al., 2010) mitochondrion, with Scer\GAL4^elav.PU (Duboff et al., 2012) neuron, with Scer\GAL4^Cha.7.4 (Nishimura et al., 2004) photoreceptor, with Scer\GAL4^GMR.PF (Wang et al., 2007) retina, with Scer\GAL4^GMR.PF (Bilen et al., 2006) sensory organ cell, with Scer\GAL4^Eq1 (Yeh et al., 2010)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	0N4R^R406W (Papanikolopoulou et al., 2010) 0N4R-R406W (Papanikolopoulou et al., 2010) h-tau(R406W) (Wang et al., 2007) hTAUR406W (Mershin et al., 2004) P{UAS-Hsap\MAPT.R406W} (FlyBase, 1996-) Tau^R406W (Khurana et al., 2006) tau^R406W (Khurana et al., 2010, Duboff et al., 2012) UAS-hTau^R406W (Yeh et al., 2010) UAS-htau^R406W (Kosmidis et al., 2010) UAS-htauR406W (Grammenoudi et al., 2006) UAS-R406W tau (Wittmann et al., 2001, Mallik and Lakhotia, 2009) UAS-R406W Tau (Malzer et al., 2010) UAS-tau.R406W (Bilen et al., 2006, Bilen and Bonini, 2007) UAS-tau(R406W) (Nishimura et al., 2004) UAS-tau^R406W (Fulga et al., 2007, Loewen and Feany, 2010, Duboff et al., 2012) uas-tau-R406W (Ryoo et al., 2007) uas-tau R406W (Ryoo et al., 2007)
Secondary FlyBase IDs

References ( 18 )
Research paper	Duboff et al., 2012, Neuron 75(4): 618--632 Tau Promotes Neurodegeneration via DRP1 Mislocalization In Vivo. [FBrf0219271] Khurana et al., 2010, PLoS Genet. 6(7): e1001026 Lysosomal dysfunction promotes cleavage and neurotoxicity of tau in vivo. [FBrf0211384] Kosmidis et al., 2010, J. Neurosci. 30(2): 464--477 Differential effects of Tau on the integrity and function of neurons essential for learning in Drosophila. [FBrf0215006] Loewen and Feany, 2010, PLoS ONE 5(9): e13084 The unfolded protein response protects from tau neurotoxicity in vivo. [FBrf0211994] Malzer et al., 2010, J. Cell Sci. 123(17): 2892--2900 Impaired tissue growth is mediated by checkpoint kinase 1 (CHK1) in the integrated stress response. [FBrf0211608] Papanikolopoulou et al., 2010, Biochem. Soc. Trans. 38(4): 981--987 Phosphorylation differentiates tau-dependent neuronal toxicity and dysfunction. [FBrf0211366] Yeh et al., 2010, Biochem. Biophys. Res. Commun. 391(1): 510--516 Drosophila notal bristle as a novel assessment tool for pathogenic study of Tau toxicity and screening of therapeutic compounds. [FBrf0209785] Mallik and Lakhotia, 2009, RNA Biol. 6(4): 464--478 RNAi for the large non-coding hsromega transcripts suppresses polyglutamine pathogenesis in Drosophila models. [FBrf0209246] Bilen and Bonini, 2007, PLoS Genet. 3(10): e177 Genome-wide screen for modifiers of ataxin-3 neurodegeneration in Drosophila. [FBrf0202746] Fulga et al., 2007, Nat. Cell Biol. 9(2): 139--148 Abnormal bundling and accumulation of F-actin mediates tau-induced neuronal degeneration in vivo. [FBrf0193954] Ryoo et al., 2007, EMBO J. 26(1): 242--252 Unfolded protein response in a Drosophila model for retinal degeneration. [FBrf0194899] Wang et al., 2007, J. Neurosci. 27(3): 574--581 Activation of PAR-1 kinase and stimulation of tau phosphorylation by diverse signals require the tumor suppressor protein LKB1. [FBrf0193064] Bilen et al., 2006, Mol. Cell 24(1): 157--163 MicroRNA pathways modulate polyglutamine-induced neurodegeneration. [FBrf0192284] Grammenoudi et al., 2006, FEBS Lett. 580(19): 4602--4606 Cell type-specific processing of human Tau proteins in Drosophila. [FBrf0194623] Khurana et al., 2006, Curr. Biol. 16(3): 230--241 TOR-mediated cell-cycle activation causes neurodegeneration in a Drosophila tauopathy model. [FBrf0190026] Mershin et al., 2004, Learn. Mem. 11(3): 277--287 Learning and memory deficits upon TAU accumulation in Drosophila mushroom body neurons. [FBrf0179337] Nishimura et al., 2004, Cell 116(5): 671--682 PAR-1 kinase plays an initiator role in a temporally ordered phosphorylation process that confers tau toxicity in Drosophila. [FBrf0174440] Wittmann et al., 2001, Science 293(5530): 711--714 Tauopathy in Drosophila: neurodegeneration without neurofibrillary tangles. [FBrf0137365]