Recombinant construct P{vas-EGFP.N}

General Information
Symbol	P{vas-EGFP.N}	FlyBase ID	FBtp0014908
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	0 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Nakamura et al., 2001)
Uses	CV term Qualifiers & info Reference characterization vas (Nakamura et al., 2001) reporter construct vital \| vas (Nakamura et al., 2001)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference molecular tag (Nakamura et al., 2001) selectable marker (Nakamura et al., 2001)
Component Alleles
Allele	vas^{T:Avic\GFP-EGFP}
Reference(s)	(Nakamura et al., 2001, Kai et al., 2005, Zhu and Xie, 2003, Kawase et al., 2004, Snapp et al., 2004, Johnstone and Lasko, 2004, Li et al., 2009, Pek and Kai, 2011, Lerit and Gavis, 2011, Sinsimer et al., 2011)
Molecular data	Construct: A germ-line specific vas promoter, 5' UTR and the sequences encoding the first 16 amino acids of vas are fused upstream of T:Avic\GFP^EGFP (EGFP) coding sequences. vas 3' UTR sequences and approximately 550bp of the vas 3' flanking region are present downstream of the Avic\GFP sequences. (Nakamura et al., 2001)
Phenotypic class
Phenotype manifest in
Other information

Allele	w^+mC
Reference(s)	(Nakamura et al., 2001)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	GFP-Vas (Lerit and Gavis, 2011) gfp-vas (Sinsimer et al., 2011) GFP-Vasa (Li et al., 2009) P[w⁺ P_vas-gfp] (Nakamura et al., 2001) P[w⁺ Pvas-gfp] (Johnstone and Lasko, 2004) P[w⁺ vas-GFP] (Snapp et al., 2004) P{vas⁺} (Johnstone and Lasko, 2004) P{vas-EGFP.N} (FlyBase, 1996-) P_vas-FL-vas (Pek and Kai, 2011) vasa-GFP (Kai et al., 2005, Kawase et al., 2004, Zhu and Xie, 2003)
Secondary FlyBase IDs

References ( 10 )
Research paper	Lerit and Gavis, 2011, Curr. Biol. 21(6): 439--448 Transport of germ plasm on astral microtubules directs germ cell development in Drosophila. [FBrf0213255] Pek and Kai, 2011, Curr. Biol. 21(1): 39--44 A role for vasa in regulating mitotic chromosome condensation in Drosophila. [FBrf0212718] Sinsimer et al., 2011, Development 138(16): 3431--3440 A late phase of germ plasm accumulation during Drosophila oogenesis requires Lost and Rumpelstiltskin. [FBrf0214542] Li et al., 2009, Cell 137(3): 509--521 Collapse of germline piRNAs in the absence of Argonaute3 reveals somatic piRNAs in flies. [FBrf0208736] Kai et al., 2005, Dev. Biol. 283(2): 486--502 The expression profile of purified Drosophila germline stem cells. [FBrf0187387] Johnstone and Lasko, 2004, Development 131(17): 4167--4178 Interaction with eIF5B is essential for Vasa function during development. [FBrf0180140] Kawase et al., 2004, Development 131(6): 1365--1375 Gbb/Bmp signaling is essential for maintaining germline stem cells and for repressing bam transcription in the Drosophila testis. [FBrf0174556] Snapp et al., 2004, Mol. Biol. Cell 15(10): 4512--4521 The fusome mediates intercellular endoplasmic reticulum connectivity in Drosophila ovarian cysts. [FBrf0180502] Zhu and Xie, 2003, Development 130(12): 2579--2588 Clonal expansion of ovarian germline stem cells during niche formation in Drosophila. [FBrf0158892] Nakamura et al., 2001, Development 128(17): 3233--3242 Me31B silences translation of oocyte-localizing RNAs through the formation of cytoplasmic RNP complex during Drosophila oogenesis. [FBrf0138368]