Recombinant construct P{UAS-EKO⁺}

General Information
Symbol	P{UAS-EKO⁺}	FlyBase ID	FBtp0014962
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	3 available
Molecular map
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (White et al., 2001)
Uses	CV term Qualifiers & info Reference binary system (targeted) Sh (White et al., 2001) characterization Sh (White et al., 2001) reporter construct vital \| Sh (White et al., 2001)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference binary component (White et al., 2001) molecular tag (White et al., 2001)
Component Alleles
Allele	Sh^{EKO.Scer\UAS.T:Avic\GFP-GL}
Reference(s)	(Rodriguez Moncalvo and Campos, 2005, Fergestad et al., 2006, Osterwalder et al., 2001, Osterwalder et al., 2001, White et al., 2001, Ishimoto et al., 2005, Luan et al., 2006, Mosca et al., 2005, White et al., 2001, Joiner et al., 2006, Peabody et al., 2008, Peabody et al., 2009, Parisky et al., 2008, Crocker et al., 2010, Rajan and Perrimon, 2012, Duch et al., 2008, Freeman et al., 2010)
Molecular data	Construct: Amino acid replacement: D316N. Amino acid replacement: K374Q. Scer\UAS sequences drive expression of a mutated form of the 29-4 Sh that has had amino acid residues 1-29 replaced with the coding sequence of T:Avic\GFP^GL. (White et al., 2001)
Phenotypic class	behavior defective \| RU486 conditional, with Scer\GAL4^{Mef2.247.Switch} (Joiner et al., 2006) body color defective \| adult stage A1, with Scer\GAL4^burs.PP (Peabody et al., 2008) body color defective \| adult stage A1, with Scer\GAL4^dimm-929 (Peabody et al., 2008) circadian behavior defective \| adult stage, with Scer\GAL4^P2.4.Pdf (Parisky et al., 2008) flight defective, with Scer\GAL4^futsch-C380, Scer\GAL80^Cha.PK (Duch et al., 2008) lethal, with Scer\GAL4^elav.PLu (Osterwalder et al., 2001) lethal, with Scer\GAL4^elav-C155 (White et al., 2001) lethal, with Scer\GAL4^how-24B (Osterwalder et al., 2001, White et al., 2001) locomotor behavior defective, with Scer\GAL4^how-24B (White et al., 2001) neurophysiology defective, with Scer\GAL4^{Mhc.Switch.PO} (Osterwalder et al., 2001) neurophysiology defective, with Scer\GAL4^NP1535 (Ishimoto et al., 2005) neurophysiology defective \| adult stage, with Scer\GAL4^futsch-C380, Scer\GAL80^Cha.PK (Duch et al., 2008) neurophysiology defective \| RU486 conditional, with Scer\GAL4^{Mhc.Switch.PO} (Osterwalder et al., 2001) sleep defective, with Scer\GAL4^Ilp2.PR (Crocker et al., 2010) taste perception defective, with Scer\GAL4^NP1535 (Ishimoto et al., 2005) visible \| cold sensitive, with Scer\GAL4^burs.PP (Peabody et al., 2008) visible \| cold sensitive, with Scer\GAL4^Ccap.PP (Peabody et al., 2008) visual behavior defective \| larval stage, with Scer\GAL4^GMR.PF (Rodriguez Moncalvo and Campos, 2005)
Phenotype manifest in	abdomen \| adult stage A1, with Scer\GAL4^burs.PP (Peabody et al., 2008) abdomen \| adult stage A1, with Scer\GAL4^dimm-929 (Peabody et al., 2008) adult cuticle, with Scer\GAL4^A307 (White et al., 2001) adult cuticle, with Scer\GAL4^Ccap.PP (Luan et al., 2006) embryonic/larval neuron, with Scer\GAL4^elav-C155 (White et al., 2001) eye, with Scer\GAL4^GMR.PF (White et al., 2001) larval head, with Scer\GAL4^Ccap.PP (Luan et al., 2006) leg, with Scer\GAL4^Ccap.PP (Luan et al., 2006) motor neuron, with Scer\GAL4^elav-C155 (White et al., 2001) photoreceptor, with Scer\GAL4^GMR.PF (White et al., 2001) presumptive embryonic/larval muscle system, with Scer\GAL4^elav-C155 (White et al., 2001) presumptive embryonic/larval muscle system, with Scer\GAL4^how-24B (White et al., 2001) synapse, with Scer\GAL4^elav-C155 (White et al., 2001) wing, with Scer\GAL4^A307 (White et al., 2001) wing, with Scer\GAL4^Ccap.PP (Luan et al., 2006, Peabody et al., 2009) wing \| adult stage A1 \| cold sensitive, with Scer\GAL4^burs.PP (Peabody et al., 2008) wing \| adult stage A1 \| cold sensitive, with Scer\GAL4^Ccap.PP (Peabody et al., 2008)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 3 )
Bloomington	40973 y¹ w^; wg^Sp-1/CyO; P{UAS-EKO⁺}323 40974 y¹ w^; P{UAS-EKO⁺}222a P{UAS-EKO⁺}222b 40975 y¹ w^*; P{UAS-EKO⁺}222a P{UAS-EKO⁺}222b; P{UAS-EKO⁺}323
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	EKO (Joiner et al., 2006, Peabody et al., 2009, Duch et al., 2008) P{UAS-EKO⁺} (FlyBase, 1996-, White et al., 2001) UAS-EKO (Luan et al., 2006, Fergestad et al., 2006, Rodriguez Moncalvo and Campos, 2005, Ishimoto et al., 2005, Osterwalder et al., 2001, Peabody et al., 2008, Parisky et al., 2008, Freeman et al., 2010) UAS-Eko (Crocker et al., 2010) UAS-EKO⁺ (Mosca et al., 2005) UAS-EkoIII (Crocker et al., 2010)
Secondary FlyBase IDs

References ( 17 )
Research paper	Rajan and Perrimon, 2012, Cell 151(1): 123--137 Drosophila cytokine unpaired 2 regulates physiological homeostasis by remotely controlling insulin secretion. [FBrf0219535] Crocker et al., 2010, Neuron 65(5): 670--681 Identification of a Neural Circuit that Underlies the Effects of Octopamine on Sleep:Wake Behavior. [FBrf0210214] Freeman et al., 2010, Brain Res. 1326: 15--29 A new genetic model of activity-induced Ras signaling dependent pre-synaptic plasticity in Drosophila. [FBrf0210453] Peabody et al., 2009, J. Neurosci. 29(11): 3343--3353 Characterization of the decision network for wing expansion in Drosophila using targeted expression of the TRPM8 channel. [FBrf0207505] Duch et al., 2008, J. Neurophysiol. 100(5): 2525--2536 Dendrite elongation and dendritic branching are affected separately by different forms of intrinsic motoneuron excitability. [FBrf0207189] Parisky et al., 2008, Neuron 60(4): 672--682 PDF cells are a GABA-responsive wake-promoting component of the Drosophila sleep circuit. [FBrf0206340] Peabody et al., 2008, J. Neurosci. 28(53): 14379--14391 Bursicon functions within the Drosophila CNS to modulate wing expansion behavior, hormone secretion, and cell death. [FBrf0206568] Fergestad et al., 2006, Genetics 172(2): 1031--1042 Neuropathology in Drosophila membrane excitability mutants. [FBrf0190718] Joiner et al., 2006, Nature 441(7094): 757--760 Sleep in Drosophila is regulated by adult mushroom bodies. [FBrf0192316] Luan et al., 2006, J. Neurosci. 26(2): 573--584 Functional dissection of a neuronal network required for cuticle tanning and wing expansion in Drosophila. [FBrf0191050] Ishimoto et al., 2005, EMBO J. 24(18): 3259--3265 G-protein gamma subunit 1 is required for sugar reception in Drosophila. [FBrf0187509] Mosca et al., 2005, Proc. Natl. Acad. Sci. U.S.A. 102(9): 3477--3482 Dissection of synaptic excitability phenotypes by using a dominant-negative Shaker K+ channel subunit. [FBrf0191470] Rodriguez Moncalvo and Campos, 2005, Dev. Biol. 286(2): 549--558 Genetic dissection of trophic interactions in the larval optic neuropil of Drosophila melanogaster. [FBrf0190156] Osterwalder et al., 2001, Proc. Natl. Acad. Sci. U.S.A. 98(22): 12596--12601 A conditional tissue-specific transgene expression system using inducible GAL4. [FBrf0139796] White et al., 2001, Neuron 31(5): 699--711 Targeted attenuation of electrical activity in Drosophila using a genetically modified K[+] channel. [FBrf0139774]
Abstract	Osterwalder et al., 2001, Bellen, Taylor, 2001: 83 A GAL4/UAS based system for conditional, tissue-specific transgene expression. [FBrf0144631] White et al., 2001, Bellen, Taylor, 2001: 62 Homeostatic changes in synaptic function following electrical inhibition of the photoreceptor response. [FBrf0144707]

Recombinant construct P{UAS-EKO+}

Recombinant construct P{UAS-EKO⁺}