Recombinant construct P{Switch1}

General Information
Symbol	P{Switch1}	FlyBase ID	FBtp0015153
Feature type	transgenic_transposon

Size	8.181	Expression data
Associated insertions	12 available
Molecular map
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Roman et al., 2001, Roman et al., 2001)
Uses	CV term Qualifiers & info Reference analytical tool inducible expression (Roman et al., 2001) carrier transposon binary system \| Scer\GAL4 (Roman et al., 2001) binary system \| ligand-inducible (Roman et al., 2001) P-element (Roman et al., 2001) removable selectable marker w[+mW.hs] (Roman et al., 2001)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent AF524838
Segments & Size
Total Size	8.181 (Roman, 2002.6.25)
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference ligand-inducible \| (Roman et al., 2001) molecular tag (Roman et al., 2001) molecular tag (Roman et al., 2001) selectable marker (Roman et al., 2001)
Component Alleles
Allele	Scer\GAL4^Switch1a
Reference(s)	(Roman et al., 2001)
Molecular data	Construct: The Scer\GAL4 DNA binding domain is fused to T:Hsap\RELA^AD and T:Hsap\PGR^LBD. (Roman et al., 2001)
Phenotypic class
Phenotype manifest in
Other information

Allele	w^+mC
Reference(s)	(Roman et al., 2001)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	P{GawB} (Roman et al., 2001) pP{Switch1} (Roman et al., 2001)
descendant(s)
Comments
	construct_comment: Scer\GAL4::Hsap\PGR::Hsap\RELA fusion (Scer\GAL4^Switch1a) protein function induced upon application of RU486 ligand. See report for T:Hsap\PGR; see report for T:Hsap\RELA.w^+mW.hs selectable marker can be removed upon exposure to P1\cre recombinase. (Roman et al., 2001)
Stocks Listed in FlyBase ( 3 )
Bloomington	6757 P{Switch1}59, w¹¹¹⁸ 8151 w¹¹¹⁸; P{Switch1}106 8527 w¹¹¹⁸; P{Switch1}bun^Switch1.32
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{Switch1} (FlyBase, 1996-, Roman et al., 2001, Roman, 2002.6.25)
Secondary FlyBase IDs

References ( 5 )
Research paper	Mathur et al., 2010, Science 327(5962): 210--213 A transient niche regulates the specification of Drosophila intestinal stem cells. [FBrf0209706] Roman et al., 2001, Proc. Natl. Acad. Sci. U.S.A. 98(22): 12602--12607 P[Switch], a system for spatial and temporal control of gene expression in Drosophila melanogaster. [FBrf0139797]
Supplementary material	Roman et al., 2001, Proc. Natl. Acad. Sci. USA 98(22): Supporting figure 6 & 7: P{Switch}, a system for spatial and temporal control of gene expression in Drosophila melanogaster. [FBrf0141774]
FlyBase analysis	FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]
DNA/RNA sequence record	Roman, 2002.6.25, GenBank/EMBL/DDBJ: AF524838 P-element cloning system vector pP{Switch1}, complete sequence. [FBrf0172536]