Recombinant construct P{bap-lacZ.3}

General Information
Symbol	P{bap-lacZ.3}	FlyBase ID	FBtp0015208
Feature type	transgenic_transposon

Size		Expression data	lacZ reporter
Associated insertions	1 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Zaffran et al., 2001)
Uses	CV term Qualifiers & info Reference reporter construct bap (Zaffran et al., 2001)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference reporter gene (Zaffran et al., 2001) selectable marker (Zaffran et al., 2001)
Component Alleles
Allele	Ecol\lacZ^bap.3
Reference(s)	(Zaffran et al., 2001, Stute et al., 2004, Kolsch and Paululat, 2002, Lee et al., 2003, Lee and Frasch, 2005, Zaffran and Frasch, 2002, Popichenko et al., 2007, Reim et al., 2012)
Molecular data	Construct: A 460bp HincII/XhoI fragment from downstream of the bap promoter is fused in reverse orientation upstream of Ecol\lacZ^hs43. (Lee and Frasch, 2005) Construct: bap enhancer sequences drive expression of Ecol\lacZ^hs43. (Zaffran et al., 2001)
Phenotypic class
Phenotype manifest in
Other information

Allele	w^+mC
Reference(s)	(Zaffran et al., 2001, Lee and Frasch, 2005)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression
distribution deduced from reporter Stage Tissue/Position (including subcellular localization) Reference embryonic stage 10 visceral mesoderm \| dorsal (Lee and Frasch, 2005) Comment:reference states trunk visceral mesoderm embryonic stage 10 -- 15 circular visceral muscle primordium \| segmentally repeated (Zaffran et al., 2001) embryonic stage 10 -- 17 visceral mesoderm \| segmentally repeated (Zaffran et al., 2001) circular visceral muscle primordium of midgut (Zaffran et al., 2001) circular visceral muscle primordium \| restricted (Zaffran et al., 2001) midgut \| restricted (Zaffran et al., 2001) embryonic stage 11 myoblast (Stute et al., 2004) visceral mesoderm (Stute et al., 2004) embryonic stage 12 visceral mesoderm (Zaffran and Frasch, 2002) embryonic stage 12 -- 13 visceral mesoderm (Lee et al., 2003) embryonic stage 12 -- 15 trunk mesoderm (Zaffran et al., 2001) embryonic stage 12 -- 16 visceral mesoderm \| segmentally repeated (Kolsch and Paululat, 2002)
Additional Information
Statement Reference Embryonic expression is observed in the trunk visceral mesoderm and, at late stages, the circular gut muscle cells. (Zaffran et al., 2001) Expression is observed in fusion-competent myoblasts at embryonic stage 11. (Stute et al., 2004)

Marker for	midgut (Zaffran et al., 2001) visceral mesoderm (Zaffran et al., 2001) myoblast visceral mesoderm (Stute et al., 2004)
Reflects expression of	bap (Kolsch and Paululat, 2002, Lee et al., 2003, Stute et al., 2004, Zaffran et al., 2001, Zaffran and Frasch, 2002)
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	bagpipe/lacZ (Kolsch and Paululat, 2002) bap3-lacZ (Lee et al., 2003, Zaffran and Frasch, 2002, Zaffran et al., 2001, Popichenko et al., 2007, Reim et al., 2012) bap-lacZ (Stute et al., 2004) P{bap3-lacZ} (FlyBase, 1996-) P{bap-lacZ.3} (FlyBase, 1996-)
Secondary FlyBase IDs

References ( 8 )
Research paper	Reim et al., 2012, Dev. Biol. 368(1): 28--43 The FGF8-related signals Pyramus and Thisbe promote pathfinding, substrate adhesion, and survival of migrating longitudinal gut muscle founder cells. [FBrf0218673] Popichenko et al., 2007, BMC Dev. Biol. 7: 49 Hand is a direct target of the forkhead transcription factor Biniou during Drosophila visceral mesoderm differentiation. [FBrf0200603] Lee and Frasch, 2005, Development 132(6): 1429--1442 Nuclear integration of positive Dpp signals, antagonistic Wg inputs and mesodermal competence factors during Drosophila visceral mesoderm induction. [FBrf0183922] Stute et al., 2004, Development 131(4): 743--754 Myoblast determination in the somatic and visceral mesoderm depends on Notch signalling as well as on milliways (mili[Alk]) as receptor for Jeb signalling. [FBrf0174588] Lee et al., 2003, Nature 425(6957): 507--512 Jelly belly protein activates the receptor tyrosine kinase Alk to specify visceral muscle pioneers. [FBrf0173097] Kolsch and Paululat, 2002, Dev. Genes Evol. 212(10): 473--485 The highly conserved cardiogenic bHLH factor Hand is specifically expressed in circular visceral muscle progenitor cells and in all cell types of the dorsal vessel during Drosophila embryogenesis. [FBrf0151908] Zaffran and Frasch, 2002, Mech. Dev. 114(1-2): 85--93 The beta3 tubulin gene is a direct target of bagpipe and biniou in the visceral mesoderm of Drosophila. [FBrf0147143] Zaffran et al., 2001, Genes Dev. 15(21): 2900--2915 biniou (FoxF), a central component in a regulatory network controlling visceral mesoderm development and midgut morphogenesis in Drosophila. [FBrf0139672]