Recombinant construct P{UAS-2xEGFP}

General Information
Symbol	P{UAS-2xEGFP}	FlyBase ID	FBtp0015221
Feature type	transgenic_transposon	Created / Updated	2002-01-30/2003-04-03

Size		Expression data
Location-dependent role		Associated insertions	7 available
Molecular map
Description & Uses
Species	synthetic construct
Description	CV term Qualifiers & info Reference transposon P-element (Osterwalder et al., 2001)
Uses	CV term Qualifiers & info Reference
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Sequence
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference binary component (Osterwalder et al., 2001)
Component Alleles
Avic\GFP^{2x.EGFP.Scer\UAS}
Reference(s)	(Osterwalder et al., 2001, Guelman et al., 2006, Osterwalder et al., 2004, Luan et al., 2006, Bhalerao et al., 2003, Halfon et al., 2002, Sen et al., 2004, Kraut and Zinn, 2004, Estrada et al., 2006, Galindo et al., 2006, Luan et al., 2006, Walters et al., 2006, Luan et al., 2006, Hueber et al., 2007)
Molecular data	Construct: Scer\UAS regulatory sequences drive expression of a dicistronic transcript which consists of two copies of the "EGFP" coding region separated by an internal ribosome entry site (IRES) from the Ubx gene. (Halfon et al., 2002)
Phenotypic class
Phenotype manifest in
Other information
w^+mC
Reference(s)	(Halfon et al., 2002, Tavsanli et al., 2004)
Molecular data	Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors. 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Minigene of the white cDNA. (Chung et al., 1993) Deletion of w^+mC 5' flanking sequences to position -305. (Qian and Pirrotta, 1995)
Phenotypic class	courtship defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^Sym.Scer\UAS, w^{a.Sym.Scer\UAS} (Giordano et al., 2002)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^Sym.Scer\UAS, w^{a.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression
Stage Tissue/Position Reference
Additional Information
Statement Reference

Assay mode
Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Synonyms & Secondary IDs
Reported As
Symbol Synonym	2XE-GFP (Bhalerao et al., 2003) 2xUAS-eGFP (Galindo et al., 2006) P{UAS-2xEGFP} (FlyBase, 1996-, Guelman et al., 2006) P{UAS-EGFP.2x} (FlyBase, 1996-) P{w+;UAS-eGFP} (Walters et al., 2006) UAS-2EGFP (Kraut and Zinn, 2004, Estrada et al., 2006) UAS-2XEGFP (Luan et al., 2006, Sen et al., 2004) UAS-2xEGFP (Luan et al., 2006, Osterwalder et al., 2004, Hueber et al., 2007) UAS-2xeGFP (Galindo et al., 2006) UAS-2xeGFP-AH2 (Galindo et al., 2006) UAS-2x enhanced green fluorescent protein (Osterwalder et al., 2001) UAS-EGFP (Luan et al., 2006) UAS-GFP (Galindo et al., 2006)
Secondary FlyBase IDs

References ( 27 )

Generate a list of	All references relating to this transgenic transposon ( 27 )

List References by type	Research paper ( 24 ) Supplementary material ( 1 ) Review ( 1 ) FlyBase analysis ( 1 )
Recent research papers ( 7 )
	Hueber et al., 2007, Development 134(2): 381--392 Comparative analysis of Hox downstream genes in Drosophila. [FBrf0193798] Estrada et al., 2006, PLoS Gen. 2(2): 160--171 An integrated strategy for analyzing the unique developmental programs of different myoblast subtypes. [FBrf0191387] Galindo et al., 2006, Proc. Natl. Acad. Sci. USA 103(36): 13439--13444 A Drosophila model of the rhabdomyosarcoma initiator PAX7-FKHR. [FBrf0192758] Guelman et al., 2006, Molec. Cell. Biol. 26(19): 7178--7189 The essential gene wda encodes a WD40 repeat subunit of Drosophila SAGA required for histone H3 acetylation. [FBrf0192338] Luan et al., 2006, J. Neurosci. 26(2): 573--584 Functional dissection of a neuronal network required for cuticle tanning and wing expansion in Drosophila. [FBrf0191050] Luan et al., 2006, Neuron 52(3): 425--436 Refined spatial manipulation of neuronal function by combinatorial restriction of transgene expression. [FBrf0193617] Walters et al., 2006, Dev. Biol. 297(2): 323--339 Planar polarization of the denticle field in the Drosophila embryo: roles for Myosin II (Zipper) and Fringe. [FBrf0200191] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2006 Show reviews ...