FlyBase Recombinant Construct Report: P{Ilp2-GAL4.R}

General Information

Symbol

P{Ilp2-GAL4.R}

FlyBase ID

FBtp0015862

Feature type

transgenic_transposable_element

Component Allele(s)

Scer\GAL4^Ilp2.PR

Also Known As

dilp2-GAL4, Ilp2-GAL4, dilp2Gal4, dilp2, dilp2G4, Dilp-Gal4

Size

Expression Data

GAL4 reporter/driver

Associated insertion(s)

1 available

Molecular map

Description and Uses

Construct components

Component allele Scer\GAL4^Ilp2.PR

Product class / Tool use(s)

Regulatory region(s)

Ilp2

Encoded product / tool

GAL4

binary expression system - driver

Description

The 859bp fragment from immediately 5' of the Ilp2 open reading frame regulates expression of a GAL4 driver.

(Rulifson et al., 2002)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Component Alleles

Allele

Scer\GAL4^Ilp2.PR

Reference(s)

(Wang et al., 2022, Arquier et al., 2021, Barber et al., 2021, Botero et al., 2021, Clements et al., 2021, de la Riva-Carrasco et al., 2021, Hasygar et al., 2021, Hückesfeld et al., 2021, Jin et al., 2021, Kamiyama et al., 2021, Landayan et al., 2021, Lund et al., 2021, Mascolo et al., 2021, Pathak et al., 2021, Qi et al., 2021, Yamazoe et al., 2021, Yoshinari et al., 2021, Zhang et al., 2021, Zhao et al., 2021, Abrieux et al., 2020, Agrawal et al., 2020, Davis et al., 2020, Liao and Nässel, 2020, Lien et al., 2020, Luo et al., 2020, Ruzzi et al., 2020, Sanaki et al., 2020, Wang et al., 2020, Wilson et al., 2020, Bhukel et al., 2019, Cassidy et al., 2019, Deng et al., 2019, Deveci et al., 2019, Dhiman et al., 2019, Drelon et al., 2019, Dreyer et al., 2019, Katsube et al., 2019, Meschi et al., 2019, Miyamoto and Amrein, 2019, Oh et al., 2019, Ryu et al., 2019, Trinh et al., 2019, Wu et al., 2019, Yurgel et al., 2019, Ashe et al., 2018, Buhler et al., 2018, de Vreede et al., 2018, Lee et al., 2018, Ohhara et al., 2018, Ren et al., 2018, Sonn et al., 2018, Valadas et al., 2018, Zandawala et al., 2018, Ziegler et al., 2018, Branch et al., 2017, Chng et al., 2017, Fedina et al., 2017, Huang et al., 2017, Kang et al., 2017, Kim et al., 2017, Martelli et al., 2017, Aditi et al., 2016, Delanoue et al., 2016, Hallier et al., 2016, Min et al., 2016, Wang et al., 2016, Wiemerslage et al., 2016, Albin et al., 2015, Hentze et al., 2015, Jeong et al., 2015, Li and Gong, 2015, Liu et al., 2015, Okamoto and Nishimura, 2015, Sano et al., 2015, Suh et al., 2015, Williams et al., 2015, Cavanaugh et al., 2014, Davis et al., 2014, Hasygar and Hietakangas, 2014, Mukherjee et al., 2014, Sakai et al., 2014, Schoofs et al., 2014, Williams et al., 2014, Williams et al., 2014, Williams et al., 2014, Bader et al., 2013, Holst et al., 2013, Kwak et al., 2013, Oh et al., 2013, Okamoto et al., 2013, Pendse et al., 2013, Subramanian et al., 2013, Hong et al., 2012, Jepson et al., 2012, Sakai et al., 2012, Yu et al., 2012, Gouzi et al., 2011, Hyun and Hashimoto, 2011, McClure et al., 2011, Sousa-Nunes et al., 2011, Stavropoulos and Young, 2011, Tennessen et al., 2011, Agrawal et al., 2010, Biteau et al., 2010, Broughton et al., 2010, Crocker et al., 2010, Sekine et al., 2010, Tsuda et al., 2010, Agrawal et al., 2009, Corl et al., 2009, Fridell et al., 2009, Jansen et al., 2009, Karpac et al., 2009, Kumar et al., 2009, Shahidullah et al., 2009, Ueishi et al., 2009, Venkiteswaran and Hasan, 2009, Zhang et al., 2009, Buch et al., 2008, Clements et al., 2008, Kaplan et al., 2008, Lee et al., 2008, Miguel-Aliaga et al., 2008, Nielsen et al., 2008, Park et al., 2008, Wang et al., 2008, Mattaliano et al., 2007, Urizar et al., 2007, Cerrato et al., 2006, Hewes et al., 2006, Park et al., 2006, Walker et al., 2006, Wang et al., 2005, Colombani et al., 2003, Rulifson et al., 2002, Rulifson et al., 2002)

Molecular data

The 859bp fragment from immediately 5' of the Ilp2 open reading frame regulates expression of a GAL4 driver.

(Rulifson et al., 2002)

Phenotype Data

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 16 -- wandering third instar larval stage

larval insulin secreting cell of pars intercerebralis

(Buhler et al., 2018)

embryonic/larval salivary gland

(Buhler et al., 2018)

larval stage -- pupal stage

larval insulin secreting cell of pars intercerebralis

(Agrawal et al., 2010)

larval stage -- adult stage

insulin secreting cell of pars intercerebralis

(Agrawal et al., 2010)

third instar larval stage

neurosecretory neuron

(Colombani et al., 2003)

brain | restricted

(Rulifson et al., 2002, Rulifson et al., 2002)

neurosecretory neuron of brain | subset

(Rulifson et al., 2002, Rulifson et al., 2002)

neurosecretory neuron | subset

(Rulifson et al., 2002, Rulifson et al., 2002)

embryonic/larval tracheal section

(Buhler et al., 2018)

pupal stage -- adult stage

adult insulin secreting cell of pars intercerebralis

(Agrawal et al., 2010)

adult stage

adult pars intercerebralis

(Davis et al., 2014, Jansen et al., 2009)

adult pars intercerebralis | restricted

(Crocker et al., 2010)

adult insulin secreting cell of pars intercerebralis | subset

(Crocker et al., 2010)

adult Dh44 neuron of pars intercerebralis

(Shahidullah et al., 2009)

Additional Information

Statement

Reference

Drives larval expression in a small cluster of insulin-producing cells in each brain lobe; processes from these neurons extend to the heart. Drives adult expression in cells of the pars intercerebralis.

(FlyBase Curators, 2017)

Scer\GAL4^Ilp2.PR-driven expression does not overlap with Ddc expression in the pupal or adult brain or larval ventral nerve cord.

(Agrawal et al., 2010)

Scer\GAL4^Ilp2.PR is expressed in insulin-like producing cells.

(Agrawal et al., 2010)

Scer\GAL4^Ilp2.PR drives expression in cells of the pars intercerebralis. Most of these cells co-express PICK1 protein.

(Jansen et al., 2009)

Larval expression is observed in a cluster of seven neurosecretory cells in each brain lobe.

(Colombani et al., 2003)

Larval expression is observed in a small cluster of insulin-producing cells in each brain lobe. Processes from these neurons extend to the heart.

(Rulifson et al., 2002)

Larval expression is observed in a small cluster of insulin-producing cells in each brain lobe.

(Rulifson et al., 2002)

Marker for

neurosecretory neuron

(Rulifson et al., 2002, Rulifson et al., 2002)

Reflects expression of

Ilp2

(Buhler et al., 2018, Davis et al., 2014, Agrawal et al., 2010, Crocker et al., 2010, Jansen et al., 2009, Colombani et al., 2003, Rulifson et al., 2002, Rulifson et al., 2002)

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

descendant(s)

Comments

Stocks (1)

Stocks containing insertion(s) of this recombinant construct

Bloomington

37516

w^*; P{Ilp2-GAL4.R}2/CyO

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (41)

Reported As

Symbol Synonym

D2G4

(Pathak et al., 2021)

DILP2-GAL4

(Dreyer et al., 2019, Zandawala et al., 2018, Mukherjee et al., 2014, Jansen et al., 2009)

DILP2-Gal4

(Barber et al., 2021, Schoofs et al., 2014, Kaplan et al., 2008)

Dilp-Gal4

(Kumar et al., 2009)

Dilp2-G4

(Cavanaugh et al., 2014)

Dilp2-GAL4

(Jin et al., 2021, Yoshinari et al., 2021, Agrawal et al., 2020, Wang et al., 2020, Meschi et al., 2019, Yurgel et al., 2019, Kim et al., 2017, Williams et al., 2015, Williams et al., 2014, Williams et al., 2014, Williams et al., 2014, Crocker et al., 2010)

Dilp2-GAL4³⁷⁵¹⁶

(Buhler et al., 2018)

Dilp2-GAL4^R

(Buhler et al., 2018)

Dilp2-GAL4^Rulifson

(Buhler et al., 2018)

Dilp2-Gal4

(Clements et al., 2021, Zhao et al., 2021, Luo et al., 2020, Sanaki et al., 2020, Deveci et al., 2019, Ryu et al., 2019, de Vreede et al., 2018, Ziegler et al., 2018, Wang et al., 2016, Liu et al., 2015, Suh et al., 2015, Bader et al., 2013, Kwak et al., 2013, Hong et al., 2012, Lee et al., 2008, Nielsen et al., 2008)

Dilp2GAL4

(Wu et al., 2019, Subramanian et al., 2013, Agrawal et al., 2010, Agrawal et al., 2009, Venkiteswaran and Hasan, 2009)

Dilp2Gal4

(Pathak et al., 2021)

Dilp2R-Gal4

(Fedina et al., 2017)

ILP2-GAL4

(Cassidy et al., 2019)

ILP2-Gal4

(Botero et al., 2021)

Ilp2-GAL4

(Lee et al., 2018, Aditi et al., 2016, Min et al., 2016, Wiemerslage et al., 2016, Sakai et al., 2014, Sousa-Nunes et al., 2011, Miguel-Aliaga et al., 2008)

Ilp2-GAL4.R

(Williams et al., 2015)

Ilp2-Gal4

(Hückesfeld et al., 2021, Landayan et al., 2021, Lund et al., 2021, Ruzzi et al., 2020, Ren et al., 2018, Sonn et al., 2018, Valadas et al., 2018)

Insulin-like peptide 2 GAL4

(Wu et al., 2019)

PI-Gal4

(Zhang et al., 2021)

P{IRP-GAL4.R}

(FlyBase, 1996-)

P{Ilp2-GAL4.R}

(Holst et al., 2013, FlyBase, 1996-)

P{ilp2-Gal4}

(Ueishi et al., 2009)

dILP2-GAL4

(Sekine et al., 2010, Walker et al., 2006)

dILP2-Gal4

(Hasygar et al., 2021, Martelli et al., 2017, Hasygar and Hietakangas, 2014, Gouzi et al., 2011)

dILP2-gal4

(Chng et al., 2017, Park et al., 2008)

dIlp2-GAL4

(Branch et al., 2017, Davis et al., 2014)

dIlp2-Gal4

(Trinh et al., 2019)

dilp-Gal4

(Albin et al., 2015)

dilp2

(Urizar et al., 2007, Cerrato et al., 2006)

dilp2 promoter

(Rulifson et al., 2002)

dilp2 promoter-GAL4

(Rulifson et al., 2002)

dilp2-GAL

(Broughton et al., 2010)

dilp2-GAL4

(Wang et al., 2022, Kamiyama et al., 2021, Qi et al., 2021, Miyamoto and Amrein, 2019, Sano et al., 2015, Pendse et al., 2013, Sakai et al., 2012, McClure et al., 2011, Tennessen et al., 2011, Corl et al., 2009, Shahidullah et al., 2009, Zhang et al., 2009, Mattaliano et al., 2007, Park et al., 2006, Colombani et al., 2003)

dilp2-gal4

(Kang et al., 2017)

dilp2G4

(Yu et al., 2012, Nielsen et al., 2008)

dilp2GAL4

(Zhang et al., 2009)

dilp2Gal4

(Ashe et al., 2018, Karpac et al., 2009, Nielsen et al., 2008, Wang et al., 2005)

ilp2-GAL4

(Dhiman et al., 2019, Wang et al., 2008)

ilp2-Gal4

(Yamazoe et al., 2021, Zhang et al., 2021, Katsube et al., 2019)

Secondary FlyBase IDs

References (139)

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