FB2020_02, released Apr 14, 2020

Recombinant Construct: P{Ilp2-GAL4.R}

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General Information
Symbol
P{Ilp2-GAL4.R}
FlyBase ID
FBtp0015862
Feature type
transgenic_transposon
Component Allele(s)
Scer\GAL4Ilp2.PR
Also Known As
dilp2-GAL4, Ilp2-GAL4, dilp2Gal4, dilp2, dilp2G4, Dilp-Gal4
Size
Expression Data
GAL4 reporter/driver
Associated insertion(s)
1 available
Molecular map
Description and Uses
Location-dependent role
Description
CV term
Qualifiers and info
Reference
transposon
P-element
(Rulifson et al., 2002)
Construct components
Component allele Scer\GAL4Ilp2.PR
Product class / Tool use(s)
Regulatory region(s)
Ilp2
Encoded product / tool
GAL4
Description

The 859bp fragment from immediately 5' of the Ilp2 open reading frame regulates expression of a GAL4 driver.

(Rulifson et al., 2002)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Features
CV term
Qualifiers and info
Reference
Component Alleles
Allele
Scer\GAL4Ilp2.PR
Reference(s)
(Bhukel et al., 2019, Deng et al., 2019, Deveci et al., 2019, Dhiman et al., 2019, Drelon et al., 2019, Dreyer et al., 2019, Meschi et al., 2019, Miyamoto and Amrein, 2019, Ryu et al., 2019, Trinh et al., 2019, Yurgel et al., 2019, Ashe et al., 2018, Buhler et al., 2018, de Vreede et al., 2018, Lee et al., 2018, Ren et al., 2018, Sonn et al., 2018, Valadas et al., 2018, Zandawala et al., 2018, Ziegler et al., 2018, Branch et al., 2017, Huang et al., 2017, Kang et al., 2017, Martelli et al., 2017, Aditi et al., 2016, Hallier et al., 2016, Min et al., 2016, Wang et al., 2016, Wiemerslage et al., 2016, Albin et al., 2015, Hentze et al., 2015, Jeong et al., 2015, Li and Gong, 2015, Liu et al., 2015, Okamoto and Nishimura, 2015, Sano et al., 2015, Suh et al., 2015, Williams et al., 2015, Cavanaugh et al., 2014, Davis et al., 2014, Hasygar and Hietakangas, 2014, Sakai et al., 2014, Schoofs et al., 2014, Williams et al., 2014, Williams et al., 2014, Williams et al., 2014, Bader et al., 2013, Holst et al., 2013, Kwak et al., 2013, Okamoto et al., 2013, Pendse et al., 2013, Subramanian et al., 2013, Hong et al., 2012, Jepson et al., 2012, Sakai et al., 2012, Yu et al., 2012, Gouzi et al., 2011, Hyun and Hashimoto, 2011, McClure et al., 2011, Sousa-Nunes et al., 2011, Stavropoulos and Young, 2011, Tennessen et al., 2011, Agrawal et al., 2010, Biteau et al., 2010, Broughton et al., 2010, Crocker et al., 2010, Sekine et al., 2010, Tsuda et al., 2010, Agrawal et al., 2009, Corl et al., 2009, Fridell et al., 2009, Jansen et al., 2009, Karpac et al., 2009, Kumar et al., 2009, Shahidullah et al., 2009, Ueishi et al., 2009, Venkiteswaran and Hasan, 2009, Zhang et al., 2009, Buch et al., 2008, Clements et al., 2008, Kaplan et al., 2008, Lee et al., 2008, Miguel-Aliaga et al., 2008, Nielsen et al., 2008, Park et al., 2008, Wang et al., 2008, Mattaliano et al., 2007, Urizar et al., 2007, Cerrato et al., 2006, Hewes et al., 2006, Park et al., 2006, Walker et al., 2006, Wang et al., 2005, Colombani et al., 2003, Rulifson et al., 2002, Rulifson et al., 2002)
Molecular data

The 859bp fragment from immediately 5' of the Ilp2 open reading frame regulates expression of a GAL4 driver.

(Rulifson et al., 2002)
Phenotype Data
Phenotypic ClassPhenotype Manifest InDetailed Description
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
third instar larval stage
neurosecretory neuron
(Colombani et al., 2003)
brain | restricted
(Rulifson et al., 2002, Rulifson et al., 2002)
neurosecretory neuron of brain | subset
(Rulifson et al., 2002, Rulifson et al., 2002)
neurosecretory neuron | subset
(Rulifson et al., 2002, Rulifson et al., 2002)
adult stage
adult pars intercerebralis
(Davis et al., 2014, Jansen et al., 2009)
adult pars intercerebralis | restricted
(Crocker et al., 2010)
adult insulin secreting cell of pars intercerebralis | subset
(Crocker et al., 2010)
adult DH44 neuron of pars intercerebralis
(Shahidullah et al., 2009)
larval stage -- adult stage
insulin secreting cell of pars intercerebralis
(Agrawal et al., 2010)
larval stage -- pupal stage
larval insulin secreting cell of pars intercerebralis
(Agrawal et al., 2010)
pupal stage -- adult stage
adult insulin secreting cell of pars intercerebralis
(Agrawal et al., 2010)
Additional Information
Statement
Reference

Drives larval expression in a small cluster of insulin-producing cells in each brain lobe; processes from these neurons extend to the heart. Drives adult expression in cells of the pars intercerebralis.

(FlyBase Curators, 2017)

Scer\GAL4Ilp2.PR-driven expression does not overlap with Ddc expression in the pupal or adult brain or larval ventral nerve cord.

(Agrawal et al., 2010)

Scer\GAL4Ilp2.PR is expressed in insulin-like producing cells.

(Agrawal et al., 2010)

Scer\GAL4Ilp2.PR drives expression in cells of the pars intercerebralis. Most of these cells co-express PICK1 protein.

(Jansen et al., 2009)

Larval expression is observed in a cluster of seven neurosecretory cells in each brain lobe.

(Colombani et al., 2003)

Larval expression is observed in a small cluster of insulin-producing cells in each brain lobe. Processes from these neurons extend to the heart.

(Rulifson et al., 2002)

Larval expression is observed in a small cluster of insulin-producing cells in each brain lobe.

(Rulifson et al., 2002)
 
Marker for
neurosecretory neuron
(Rulifson et al., 2002, Rulifson et al., 2002)
Reflects expression of
Ilp2
(Davis et al., 2014, Agrawal et al., 2010, Crocker et al., 2010, Jansen et al., 2009, Colombani et al., 2003, Rulifson et al., 2002, Rulifson et al., 2002)
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (1)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (33)
Reported As
Symbol Synonym
DILP2-GAL4
(Dreyer et al., 2019, Zandawala et al., 2018, Jansen et al., 2009)
DILP2-Gal4
(Schoofs et al., 2014, Kaplan et al., 2008)
Dilp-Gal4
(Kumar et al., 2009)
Dilp2-G4
(Cavanaugh et al., 2014)
Dilp2-GAL4
(Meschi et al., 2019, Yurgel et al., 2019, Williams et al., 2015, Williams et al., 2014, Williams et al., 2014, Williams et al., 2014, Crocker et al., 2010)
Dilp2-GAL437516
(Buhler et al., 2018)
Dilp2-GAL4R
(Buhler et al., 2018)
Dilp2-GAL4Rulifson
(Buhler et al., 2018)
Dilp2-Gal4
(Deveci et al., 2019, Ryu et al., 2019, de Vreede et al., 2018, Ziegler et al., 2018, Wang et al., 2016, Liu et al., 2015, Suh et al., 2015, Bader et al., 2013, Kwak et al., 2013, Hong et al., 2012, Lee et al., 2008, Nielsen et al., 2008)
Dilp2GAL4
(Subramanian et al., 2013, Agrawal et al., 2010, Agrawal et al., 2009, Venkiteswaran and Hasan, 2009)
Ilp2-GAL4
(Lee et al., 2018, Aditi et al., 2016, Min et al., 2016, Wiemerslage et al., 2016, Sakai et al., 2014, Sousa-Nunes et al., 2011, Miguel-Aliaga et al., 2008)
Ilp2-GAL4.R
(Williams et al., 2015)
Ilp2-Gal4
(Ren et al., 2018, Sonn et al., 2018, Valadas et al., 2018)
P{IRP-GAL4.R}
(FlyBase, 1996-)
P{Ilp2-GAL4.R}
(Holst et al., 2013, FlyBase, 1996-)
P{ilp2-Gal4}
(Ueishi et al., 2009)
dILP2-GAL4
(Sekine et al., 2010, Walker et al., 2006)
dILP2-Gal4
(Martelli et al., 2017, Hasygar and Hietakangas, 2014, Gouzi et al., 2011)
dILP2-gal4
(Park et al., 2008)
dIlp2-GAL4
(Branch et al., 2017, Davis et al., 2014)
dIlp2-Gal4
(Trinh et al., 2019)
dilp-Gal4
(Albin et al., 2015)
dilp2
(Urizar et al., 2007, Cerrato et al., 2006)
dilp2 promoter
(Rulifson et al., 2002)
dilp2 promoter-GAL4
(Rulifson et al., 2002)
dilp2-GAL
(Broughton et al., 2010)
dilp2-GAL4
(Miyamoto and Amrein, 2019, Sano et al., 2015, Pendse et al., 2013, Sakai et al., 2012, McClure et al., 2011, Tennessen et al., 2011, Corl et al., 2009, Shahidullah et al., 2009, Zhang et al., 2009, Mattaliano et al., 2007, Park et al., 2006, Colombani et al., 2003)
dilp2-Gal4
(Bhukel et al., 2019, Deng et al., 2019, Drelon et al., 2019, Huang et al., 2017, Hallier et al., 2016, Hentze et al., 2015, Jeong et al., 2015, Li and Gong, 2015, Okamoto and Nishimura, 2015, Okamoto et al., 2013, Jepson et al., 2012, Yu et al., 2012, Hyun and Hashimoto, 2011, Biteau et al., 2010, Tsuda et al., 2010, Fridell et al., 2009, Buch et al., 2008, Clements et al., 2008, Hewes et al., 2006)
dilp2-gal4
(Kang et al., 2017)
dilp2G4
(Yu et al., 2012, Nielsen et al., 2008)
dilp2GAL4
(Zhang et al., 2009)
dilp2Gal4
(Ashe et al., 2018, Karpac et al., 2009, Nielsen et al., 2008, Wang et al., 2005)
ilp2-GAL4
(Dhiman et al., 2019, Wang et al., 2008)
Secondary FlyBase IDs
    References (98)