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Recombinant Construct: P{EPgy2}

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General Information
Symbol
P{EPgy2}
FlyBase ID
FBtp0015947
Feature type
transgenic_transposon
Component Allele(s)
Also Known As
EY
Size
10.908
Expression Data
Associated insertion(s)
3635 available
Molecular map
Description and Uses
Location-dependent role
mobile activating element (UASG)
(Bellen et al., 2004)
Description
CV term
Qualifiers and info
Reference
transposon
P-element
(Bellen et al., 2004)
Construct components
Product class / Tool use(s)
Regulatory region(s)
UASp
Encoded product / tool
Marker(s)
w+mC
y+mDint2
Description
The P{EPgy2} element contains UASp regulatory sequences at its 3' end, oriented oriented so that in the presence of a GAL4 driver, transcription from UASp is directed outwards from this end of the element. This can result in misexpression of neighbouring genomic loci when the element is inserted in the genome. P{EPgy2} is similar to the P{EPg} element, with the major difference being the inclusion of y+mDint2 (between the w+mC marker and the UASp sequences) and the removal of the plasmid rescue module.
(Levis, 2002.11.20)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
10.908
Left end
P5'
(Levis, 2002.11.20)
Right end
P3'
(Levis, 2002.11.20)
 
Segments
Num
Orientation
Symbol
1
+
CaSpeR_P5'(P5',Sp)
2
+
001268
3
+
001331
4
-
y+mDint25.2(S,S)
5
+
001332
6
+
GAGA_sites
7
+
001271
8
+
14xUASGP\T
9
+
001335
10
+
EPg_P3'(r,P3')
Reference
(Levis, 2002.11.20)
Features
CV term
Qualifiers and info
Reference
3\'
terminus
(Levis, 2002.11.20)
mobile
activating
(Levis, 2002.11.20)
partial
rescue
(Pirrotta, 1988)
promoter
|
(Levis, 2002.11.20)
regulatory/structural
DNA
(Rorth, 1996)
selectable
marker
(Pirrotta, 1988)
selectable
marker
(Patton et al., 1992)
Component Alleles
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
Comments
Additional information at: GDP Transposons http://flypush.imgen.bcm.tmc.edu/pscreen/transposons.html
(Bellen et al., 2004)
Stocks (3,215)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
References (53)