Recombinant construct P{wHy}

General Information
Symbol	P{wHy}	FlyBase ID	FBtp0016141
Feature type	transgenic_transposon

Size	15.588	Expression data
Associated insertions	825 available
Molecular map
Recent Updates
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FB2011_10
FB2012_01	References Myrick et al., 2005.5.16, Deletion Generator Project genomic insertion collection.
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Description & Uses
Species	synthetic construct
Location-dependent role	carries hobo element (Huet et al., 2002) deletion generation component (Huet et al., 2002)
Description	CV term Qualifiers & info Reference transposon H-element (Huet et al., 2002) P-element (Huet et al., 2002, Mohr and Gelbart, 2002, Huet and Gelbart, 2002.11, Bellen et al., 2004)
Uses	CV term Qualifiers & info Reference genomic insertion screen mutagenesis deletions (Huet et al., 2002, Mohr and Gelbart, 2002)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)	FlyBase-compiled
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent AF516513
Segments & Size
Total Size	15.588
Left end	P5\' (Huet et al., 2002)
Right end	P3\' (Huet et al., 2002)

Segments	Number Orientation Symbol 1 + CaSpeR_P5'(P5',Sp) 2 + 001299 3 + 94E-0.034 4 + hobo5'(H5',R1) 5 + EcoRI 6 + hobo3'(R1,H3') 7 + 94E-0.4 8 + 001300 9 - y⁺7.7::y⁺3'(S) 10 + 001301 11 + Car1_P3'(H3,P3')
Reference	(Huet et al., 2002)
Features
	CV term Qualifiers & info Reference complete rescue (Basler and Struhl, 1994) partial rescue (Pirrotta, 1988) selectable marker (Pirrotta, 1988) selectable marker (Huet et al., 2002) selectable marker (Huet et al., 2002) visible marker (Geyer and Corces, 1987)
Component Alleles
Allele	w^+mC
Reference(s)	(Pirrotta, 1988, Huet et al., 2002)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information

Allele	y^+t7.7
Reference(s)	(Basler and Struhl, 1994, Geyer and Corces, 1987, Huet et al., 2002)
Molecular data	Construct: 8kb SalI fragment carrying y⁺. (Merriam, 1997.5.9) Contained within 7.7kb SalI-BglII fragment that includes the entire y⁺ gene. (Struhl and Basler, 1993) Fragment containing the y gene, including 2873bp 5' and 130bp 3' flanking sequences. (Harrison et al., 1989) 5' regulatory region deletion derivative of y, 2873 nucleotides remain upstream of the transcription start site. (Geyer and Corces, 1987)
Phenotypic class	wild-type (Geyer and Corces, 1987, Harrison et al., 1989, Benedyk et al., 1994)
Phenotype manifest in
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Assay mode
Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	pP{wHy} (Huet et al., 2002)
descendant(s)	H{FL1-R1D} (Huet et al., 2002) P{3'wHy} (Huet and Gelbart, 2002.11, Mohr and Gelbart, 2002) P{5'wHy} (Huet and Gelbart, 2002.11, Mohr and Gelbart, 2002)
Comments
	report comment: See GenBank accession AF516513. (Huet et al., 2002) construct_comment: Exposure to source of H\T (hobo elementtransposase) results in efficient generation of deletions with one endpoint atsite of P{wHy} insertion. (Mohr and Gelbart, 2002)
Stocks Listed in FlyBase ( 831 )
Bloomington	21040 y¹ w^67c23 P{wHy}DG05201 17334 y¹ w^67c23; P{wHy}DG07106 17370 y¹ w^67c23; P{wHy}DG07205 17379 y¹ w^67c23; P{wHy}DG07710 17384 y¹ w^67c23; P{wHy}DG07206 17431 y¹ w^67c23; P{wHy}DG07301 17499 y¹ w^67c23; P{wHy}DG07612 17572 y¹ w^67c23; P{wHy}DG07810 20371 y¹ w^67c23; P{wHy}DG04111 20386 y¹ w^67c23; P{wHy}DG04605 20388 y¹ w^67c23; P{wHy}DG04609 20395 y¹ w^67c23; P{wHy}DG04711
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External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{wHy} (FlyBase, 1996-, Baugh, 2002.5.30, Kornberg, 2002, Bellen et al., 2004, Mohr and Gelbart, 2002, Huet and Gelbart, 2002.11, Huet et al., 2002)
Secondary FlyBase IDs

References ( 28 )

Generate a list of	All references relating to this transgenic transposon ( 28 )

List References by type	Research paper ( 18 ) Review ( 2 ) Personal communication to FlyBase ( 4 ) FlyBase analysis ( 3 ) DNA/RNA sequence record ( 1 )
Recent research papers (0)
	All research papers listed in FlyBase were published before 2010 Show research papers ...
Recent reviews (0)
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