Recombinant construct P{wHy}

General Information
Symbol	P{wHy}	FlyBase ID	FBtp0016141
Feature type	transgenic_transposon

Size	15.588	Expression data
Associated insertions	825 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role	carries hobo element (Huet et al., 2002) deletion generation component (Huet et al., 2002)
Description	CV term Qualifiers & info Reference transposon H-element (Huet et al., 2002) P-element (Huet et al., 2002, Mohr and Gelbart, 2002, Huet and Gelbart, 2002.11, Bellen et al., 2004)
Uses	CV term Qualifiers & info Reference genomic insertion screen mutagenesis deletions (Huet et al., 2002, Mohr and Gelbart, 2002)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)	FlyBase-compiled
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent AF516513
Segments & Size
Total Size	15.588
Left end	P5\' (Huet et al., 2002)
Right end	P3\' (Huet et al., 2002)

Segments	Number Orientation Symbol 1 + CaSpeR_P5'(P5',Sp) 2 + 001299 3 + 94E-0.034 4 + hobo5'(H5',R1) 5 + EcoRI 6 + hobo3'(R1,H3') 7 + 94E-0.4 8 + 001300 9 - y⁺7.7::y⁺3'(S) 10 + 001301 11 + Car1_P3'(H3,P3')
Reference	(Huet et al., 2002)
Features
	CV term Qualifiers & info Reference complete rescue (Basler and Struhl, 1994) partial rescue (Pirrotta, 1988) selectable marker (Pirrotta, 1988) selectable marker (Huet et al., 2002) selectable marker (Huet et al., 2002) visible marker (Geyer and Corces, 1987)
Component Alleles
Allele	w^+mC
Reference(s)	(Pirrotta, 1988, Huet et al., 2002)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information

Allele	y^+t7.7
Reference(s)	(Basler and Struhl, 1994, Geyer and Corces, 1987, Huet et al., 2002)
Molecular data	Construct: 8kb SalI fragment carrying y⁺. (Merriam, 1997.5.9) Contained within 7.7kb SalI-BglII fragment that includes the entire y⁺ gene. (Struhl and Basler, 1993) Fragment containing the y gene, including 2873bp 5' and 130bp 3' flanking sequences. (Harrison et al., 1989) 5' regulatory region deletion derivative of y, 2873 nucleotides remain upstream of the transcription start site. (Geyer and Corces, 1987)
Phenotypic class	wild-type (Benedyk et al., 1994, Geyer and Corces, 1987, Harrison et al., 1989)
Phenotype manifest in
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	pP{wHy} (Huet et al., 2002)
descendant(s)	H{FL1-R1D} (Huet et al., 2002) P{3'wHy} (Huet and Gelbart, 2002.11, Mohr and Gelbart, 2002) P{5'wHy} (Huet and Gelbart, 2002.11, Mohr and Gelbart, 2002)
Comments
	Additional information at: GDP Transposons http://flypush.imgen.bcm.tmc.edu/pscreen/transposons.html (Bellen et al., 2004) report comment: See GenBank accession AF516513. (Huet et al., 2002) construct_comment: Exposure to source of H\T (hobo elementtransposase) results in efficient generation of deletions with one endpoint atsite of P{wHy} insertion. (Mohr and Gelbart, 2002)
Stocks Listed in FlyBase ( 159 )
Bloomington	17499 y¹ w^67c23; P{wHy}DG07612 21249 y¹ w^67c23; P{wHy}DG24411 21320 y¹ w^67c23; P{wHy}DG30409 21540 y¹ w^67c23; P{wHy}DG16502 21548 y¹ w^67c23; P{wHy}DG16607 21663 y¹ w^67c23; P{wHy}DG08511 21686 y¹ w^67c23; P{wHy}DG19401 21736 y¹ w^67c23; P{wHy}DG20507 21776 y¹ w^67c23; P{wHy}DG39210 21778 y¹ w^67c23; P{wHy}DG39301 22276 y¹ w^67c23; P{wHy}DG20404 22277 y¹ w^67c23; P{wHy}DG20510
	More stocks available...
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{wHy} (FlyBase, 1996-, Baugh, 2002.5.30, Kornberg, 2002, Bellen et al., 2004, Mohr and Gelbart, 2002, Huet and Gelbart, 2002.11, Huet et al., 2002)
Secondary FlyBase IDs

References ( 9 )
Research paper	Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Huet et al., 2002, Proc. Natl. Acad. Sci. U.S.A. 99(15): 9948--9953 A deletion-generator compound element allows deletion saturation analysis for genomewide phenotypic annotation. [FBrf0151478] Mohr and Gelbart, 2002, Genetics 162(1): 165--176 Using the P{wHy} hybrid transposable element to disrupt genes in region 54D-55B in Drosophila melanogaster. [FBrf0152030]
Review	Kornberg, 2002, Proc. Natl. Acad. Sci. U.S.A. 99(15): 9607--9608 Another arrow in the Drosophila quiver. [FBrf0151476]
Personal communication to FlyBase	Myrick et al., 2005.5.16, Deletion Generator Project genomic insertion collection. Deletion Generator Project genomic insertion collection. [FBrf0188490] Huet and Gelbart, 2002.11, Huet et al. deletions. Huet et al. deletions. [FBrf0155386]
FlyBase analysis	FlyBase, 2007.6, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0199566] FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]
DNA/RNA sequence record	Baugh, 2002.5.30, GenBank/EMBL/DDBJ: AF516513 A deletion-generator compound element allows deletion saturation analysis for genomewide phenotypic annotation. [FBrf0153944]