Recombinant construct P{UAS-syt.eGFP}

General Information
Symbol	P{UAS-syt.eGFP}	FlyBase ID	FBtp0016185
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	3 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Zhang et al., 2002)
Uses	CV term Qualifiers & info Reference binary system (targeted) syt (Zhang et al., 2002) characterization syt (Zhang et al., 2002) reporter construct vital \| syt (Zhang et al., 2002)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference binary component (Zhang et al., 2002) molecular tag (Zhang et al., 2002) selectable marker (Zhang et al., 2002)
Component Alleles
Allele	Syt1^{Scer\UAS.T:Avic\GFP-EGFP}
Reference(s)	(Zhang et al., 2002, Zhang et al., 2007, Murthy et al., 2003, Iyengar et al., 2006, Ang et al., 2006, Wang et al., 2007, Lorenzo et al., 2010, Wu et al., 2011, Morikawa et al., 2011, Lai et al., 2012, Chen et al., 2012, Koon et al., 2011, Hartl et al., 2011, Barber et al., 2009, Ting et al., 2007, Vömel and Wegener, 2008, Iijima-Ando et al., 2009, Besse et al., 2007, Clements et al., 2008, Li et al., 2009, Nicolaï et al., 2010, Mao and Davis, 2009, Nicolaï et al., 2010, Liu et al., 2010, Roy et al., 2007, Liu et al., 2011, Shih and Chiang, 2011, Eddison et al., 2011, Christiansen et al., 2011, Tong et al., 2011, Séjourné et al., 2011, Neckameyer and Bhatt, 2012, Crocker et al., 2010, Jansen et al., 2009, Lloyd et al., 2012, Miller et al., 2008, Liu et al., 2010, Gong et al., 2010, Lam et al., 2010)
Molecular data	Construct: Scer\UAS regulatory sequences drive expression of syt fused to "eGFP" (T:Avic\GFP^EGFP). (Zhang et al., 2002)
Phenotypic class	viable, with Scer\GAL4^4G (Zhang et al., 2002)
Phenotype manifest in
Other information

Allele	w^+mC
Reference(s)	(Zhang et al., 2002)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 6 )
Bloomington	6924 P{UAS-syt.eGFP}1, w^* 6925 w^; P{UAS-syt.eGFP}2 6926 w^; P{UAS-syt.eGFP}3 6923 P{GawB}elav^C155, P{UAS-syt.eGFP}1, w^* 33065 w¹¹¹⁸; L¹/CyO; P{UAS-DenMark}2, P{UAS-syt.eGFP}3 33064 w¹¹¹⁸; P{UAS-DenMark}2, P{UAS-syt.eGFP}2; In(3L)D, mirr^SaiD1 D¹/TM6C, Sb¹
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{UAS-syt.eGFP} (FlyBase, 1996-) Synaptotagmin::GFP (Ang et al., 2006) synaptotagmin.eGFP (Mao and Davis, 2009) synaptotagmin-GFP (Morikawa et al., 2011, Miller et al., 2008) Synaptotagmin-GFP (Miller et al., 2008) Syt 1-GFP (Barber et al., 2009) syt::GFP (Chen et al., 2012) Syt::GFP (Nicolaï et al., 2010, Séjourné et al., 2011) Syt:GFP (Lloyd et al., 2012) syt-eGFP (Zhang et al., 2002) Syt-eGFP (Liu et al., 2010) syt-GFP (Lorenzo et al., 2010, Iijima-Ando et al., 2009) SytGFP (Vömel and Wegener, 2008) Syt-GFP (Miller et al., 2008, Gong et al., 2010) UAS:syt-GFP (Wu et al., 2011) UAS-eGFP (Lam et al., 2010) UAS-GFP syt (Crocker et al., 2010) UAS-Synaptotagmin GFP (Tong et al., 2011) UAS-Syt1-GFP (Koon et al., 2011) UAS-syt::GFP (Nicolaï et al., 2010, Lai et al., 2012) UAS-Syt::GFP (Shih and Chiang, 2011) UAS-syt.eGFP (Mao and Davis, 2009, Neckameyer and Bhatt, 2012, Jansen et al., 2009) UAS-syt^GFP (Christiansen et al., 2011) UAS-syt-EGFP (Wang et al., 2007, Besse et al., 2007) UAS-syt-eGFP (Ting et al., 2007) UAS-Syt-eGFP (Liu et al., 2011) UAS-Syt-GFP (Murthy et al., 2003, Li et al., 2009, Morikawa et al., 2011, Iyengar et al., 2006, Roy et al., 2007) uas-SytGFP (Vömel and Wegener, 2008) UAS-syt-GFP (Iijima-Ando et al., 2009, Clements et al., 2008, Eddison et al., 2011, Liu et al., 2010) UAS-SytGFP (Tong et al., 2011) UAS-sytGFP (Hartl et al., 2011)
Secondary FlyBase IDs

References ( 39 )

Generate a list of	All references relating to this transgenic transposon ( 39 )

List References by type	Research paper ( 37 ) Supplementary material ( 2 )
Recent research papers ( 14 )
	Chen et al., 2012, Science 335(6069): 678--685 Visualizing long-term memory formation in two neurons of the Drosophila brain. [FBrf0217453] Lai et al., 2012, Proc. Natl. Acad. Sci. U.S.A. 109(7): 2607--2612 Auditory circuit in the Drosophila brain. [FBrf0217491] Lloyd et al., 2012, Neuron 74(2): 344--360 The p150(Glued) CAP-Gly Domain Regulates Initiation of Retrograde Transport at Synaptic Termini. [FBrf0218160] Neckameyer and Bhatt, 2012, BMC Neurosci. 13: 26 Neurotrophic actions of dopamine on the development of a serotonergic feeding circuit in Drosophila melanogaster. [FBrf0218484] Christiansen et al., 2011, J. Neurosci. 31(26): 9696--9707 Presynapses in Kenyon Cell Dendrites in the Mushroom Body Calyx of Drosophila. [FBrf0214059] Eddison et al., 2011, Neuron 70(5): 979--990 arouser Reveals a Role for Synapse Number in the Regulation of Ethanol Sensitivity. [FBrf0213908] Hartl et al., 2011, J. Neurosci. 31(44): 15660--15673 A New Prospero and microRNA-279 Pathway Restricts CO2 Receptor Neuron Formation. [FBrf0216631] Koon et al., 2011, Nat. Neurosci. 14(2): 190--199 Autoregulatory and paracrine control of synaptic and behavioral plasticity by octopaminergic signaling. [FBrf0212895] Liu et al., 2011, J. Neurosci. 31(6): 2052--2063 Drosophila Acyl-CoA Synthetase Long-Chain Family Member 4 Regulates Axonal Transport of Synaptic Vesicles and Is Required for Synaptic Development and Transmission. [FBrf0212968] Morikawa et al., 2011, Proc. Natl. Acad. Sci. U.S.A. 108(48): 19389--19394 Different levels of the Tripartite motif protein, Anomalies in sensory axon patterning (Asap), regulate distinct axonal projections of Drosophila sensory neurons. [FBrf0216734] Shih and Chiang, 2011, J. Neurogenet. 25(1-2): 1--6 Anatomical Characterization of Thermosensory AC Neurons in the Adult Drosophila Brain. [FBrf0213700] Séjourné et al., 2011, Nat. Neurosci. 14(7): 903--910 Mushroom body efferent neurons responsible for aversive olfactory memory retrieval in Drosophila. [FBrf0214029] Tong et al., 2011, Neuron 71(3): 447--459 Rich Regulates Target Specificity of Photoreceptor Cells and N-Cadherin Trafficking in the Drosophila Visual System via Rab6. [FBrf0214681] Wu et al., 2011, Neuron 70(2): 281--298 A combinatorial semaphorin code instructs the initial steps of sensory circuit assembly in the Drosophila CNS. [FBrf0213571] Show all research papers ...