Recombinant construct P{UAS-nec.L-dsRNA}

General Information
Symbol	P{UAS-nec.L-dsRNA}	FlyBase ID	FBtp0016223
Feature type	transgenic_transposon	Created / Updated	2003-04-03/2003-04-03

Size		Expression data
Location-dependent role		Associated insertions	0 available
Molecular map
Description & Uses
Species	synthetic construct
Description	CV term Qualifiers & info Reference transposon P-element (Reichhart et al., 2002)
Uses	CV term Qualifiers & info Reference characterization nec (Reichhart et al., 2002) binary system (targeted) nec (Reichhart et al., 2002) RNAi (dsRNA) construct nec (Reichhart et al., 2002)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Sequence
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference binary component (Reichhart et al., 2002)
Component Alleles
nec^{dsRNA.L.Scer\UAS}
Reference(s)	(Reichhart et al., 2002, Kambris et al., 2006, Kambris et al., 2006)
Molecular data	Construct: Scer\UAS regulatory sequences drive expression of 566bp antisense and sense nec fragments which are separated by mub^816.i, an 816bp spacer fragment derived from an intron of mub. (Reichhart et al., 2002)
Phenotypic class	melanotic necrosis, with Scer\GAL4^Act5C.PI (Reichhart et al., 2002)
Phenotype manifest in
Other information
mub^816.i
Reference(s)	(Reichhart, 2004.8)
Molecular data	Construct: Intronic fragment of mub used in generating most of the UAS-dsRNA transgenic constructs in FBrf0152370. (Reichhart, 2004.8)
Phenotypic class
Phenotype manifest in
Other information
Expression Data
Reporter Expression
Stage Tissue/Position Reference
Additional Information
Statement Reference

Assay mode
Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{UAS-nec.L-dsRNA} (FlyBase, 1996-) UAS-nec-IR (Kambris et al., 2006, Kambris et al., 2006)
Secondary FlyBase IDs

References ( 5 )
Research paper	Kambris et al., 2006, Curr. Biol. 16(8): 808--813 Drosophila immunity: a large-scale in vivo RNAi screen identifies five serine proteases required for Toll activation. [FBrf0190023] Reichhart et al., 2002, genesis 34(1-2): 160--164 Splice-activated UAS hairpin vector gives complete RNAi knockout of single or double target transcripts in Drosophila melanogaster. [FBrf0152370]
Supplementary material	Kambris et al., 2006, Curr. Biol. 16(8): Document S1. Two Figures and Supplemental Experimental Procedures. [FBrf0199186]
Personal communication to FlyBase	Reichhart, 2004.8, pJM1084. pJM1084. [FBrf0178906]
FlyBase analysis	FlyBase, 1996-, FlyBase inference based on genome sequence analysis. FlyBase inference based on genome sequence analysis. [FBrf0104946]