Recombinant construct P{UAS-n-syb.eGFP}

General Information
Symbol	P{UAS-n-syb.eGFP}	FlyBase ID	FBtp0016225
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	3 available
Molecular map
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Zhang et al., 2002)
Uses	CV term Qualifiers & info Reference binary system (targeted) n-syb (Zhang et al., 2002) characterization n-syb (Zhang et al., 2002) reporter construct vital \| n-syb (Zhang et al., 2002)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference binary component (Zhang et al., 2002) molecular tag (Zhang et al., 2002) selectable marker (Zhang et al., 2002)
Component Alleles
Allele	n-syb^{Scer\UAS.T:Avic\GFP-EGFP}
Reference(s)	(Sykes and Condron, 2005, Zhang et al., 2002, Lorenzo et al., 2010, Kolodziejczyk et al., 2008, Thorne and Amrein, 2008, Hamasaka et al., 2005, Petersen and Stowers, 2011, Jansen et al., 2009, Kong et al., 2010, Carlsson et al., 2010, Hamasaka and Nassel, 2006)
Molecular data	Construct: Scer\UAS regulatory sequences drive expression of n-syb fused to "eGFP" (T:Avic\GFP^EGFP). (Zhang et al., 2002)
Phenotypic class	lethal \| pupal stage, with Scer\GAL4^4G (Zhang et al., 2002)
Phenotype manifest in
Other information

Allele	w^+mC
Reference(s)	(Zhang et al., 2002)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 3 )
Bloomington	6921 w^; P{UAS-n-syb.eGFP}2 6922 w^; P{UAS-n-syb.eGFP}3 6920 P{GawB}elav^C155, P{UAS-n-syb.eGFP}1, w^*/FM7a
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	n-syb-GFP (Lorenzo et al., 2010) P{UAS-n-syb.eGFP} (FlyBase, 1996-) P{UAS-n-syb.egfp} (Hamasaka and Nassel, 2006) syb-eGFP (Zhang et al., 2002) UAS-nSyb::GFP (Carlsson et al., 2010) UAS-nsyb.egfp (Kolodziejczyk et al., 2008) UAS-n-syb.eGFP (Jansen et al., 2009) UAS-nsybEGFP (Thorne and Amrein, 2008) UAS-n-syb-egfp (Kong et al., 2010) UAS-n-syb-GFP (Petersen and Stowers, 2011) UAS-syb-GFP (Sykes and Condron, 2005)
Secondary FlyBase IDs

References ( 11 )
Research paper	Petersen and Stowers, 2011, PLoS ONE 6(9): e24531 A Gateway MultiSite Recombination Cloning Toolkit. [FBrf0215584] Carlsson et al., 2010, J. Comp. Neurol. 518(16): 3359--3380 Multiple neuropeptides in the Drosophila antennal lobe suggest complex modulatory circuits. [FBrf0211156] Kong et al., 2010, PLoS ONE 5(4): e9954 A pair of dopamine neurons target the D1-like dopamine receptor DopR in the central complex to promote ethanol-stimulated locomotion in Drosophila. [FBrf0210514] Lorenzo et al., 2010, J. Cell Biol. 189(1): 143--158 Spectrin mutations that cause spinocerebellar ataxia type 5 impair axonal transport and induce neurodegeneration in Drosophila. [FBrf0210445] Jansen et al., 2009, J. Comp. Neurol. 517(3): 313--332 PICK1 expression in the Drosophila central nervous system primarily occurs in the neuroendocrine system. [FBrf0208848] Kolodziejczyk et al., 2008, PLoS ONE 3(5): e2110 Glutamate, GABA and acetylcholine signaling components in the lamina of the Drosophila visual system. [FBrf0210240] Thorne and Amrein, 2008, J. Comp. Neurol. 506(4): 548--568 Atypical expression of Drosophila gustatory receptor genes in sensory and central neurons. [FBrf0201488] Hamasaka and Nassel, 2006, J. Comp. Neurol. 494(2): 314--330 Mapping of serotonin, dopamine, and histamine in relation to different clock neurons in the brain of Drosophila. [FBrf0191078] Hamasaka et al., 2005, J. Neurobiol. 65(3): 225--240 GABA modulates Drosophila circadian clock neurons via GABA(B) receptors and decreases in calcium. [FBrf0191024] Sykes and Condron, 2005, Dev. Biol. 286(1): 207--216 Development and sensitivity to serotonin of Drosophila serotonergic varicosities in the central nervous system. [FBrf0190167] Zhang et al., 2002, genesis 34(1-2): 142--145 Living synaptic vesicle marker: synaptotagmin-GFP. [FBrf0152366]