Recombinant construct P{UAS-f::nec-dsRNA}

General Information
Symbol	P{UAS-f::nec-dsRNA}	FlyBase ID	FBtp0016255
Feature type	transgenic_transposon	Created / Updated	2003-04-03/2003-04-03

Size		Expression data
Location-dependent role		Associated insertions	4 available
Molecular map
Description & Uses
Species	synthetic construct
Description	CV term Qualifiers & info Reference transposon P-element (Reichhart et al., 2002)
Uses	CV term Qualifiers & info Reference characterization f \| fusion construct (Reichhart et al., 2002) nec \| fusion construct (Reichhart et al., 2002)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Sequence
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference chimeric protein (Reichhart et al., 2002)
Component Alleles
f::nec^{dsRNA.Scer\UAS}
Reference(s)	(Reichhart et al., 2002)
Molecular data	Construct: Scer\UAS regulatory sequences drive expression of a 601bp antisense f fragment followed by a 566bp antisense nec fragment, followed by mub^816.i, an 816bp spacer fragment derived from an intron of mub, followed by a 566bp sense nec fragment followed by a 601bp sense f fragment. (Reichhart et al., 2002)
Phenotypic class	melanotic necrosis, with Scer\GAL4^Act5C.PI (Reichhart et al., 2002)
Phenotype manifest in	wing hair, with Scer\GAL4^Act5C.PI (Reichhart et al., 2002) chaeta, with Scer\GAL4^Act5C.PI (Reichhart et al., 2002) chaeta, with Scer\GAL4^ptc-559.1 (Reichhart et al., 2002) chaeta, with Scer\GAL4^VNC.ap (Reichhart et al., 2002) chaeta, with Scer\GAL4^Dll-md23 (Reichhart et al., 2002) chaeta, with Scer\GAL4^dpp.blk1 (Reichhart et al., 2002)
Other information
mub^816.i
Reference(s)	(Reichhart, 2004.8)
Molecular data	Construct: Intronic fragment of mub used in generating most of the UAS-dsRNA transgenic constructs in FBrf0152370. (Reichhart, 2004.8)
Phenotypic class
Phenotype manifest in
Other information
Expression Data
Reporter Expression
Stage Tissue/Position Reference
Additional Information
Statement Reference

Assay mode
Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{UAS-f::nec-dsRNA} (FlyBase, 1996-)
Secondary FlyBase IDs

References ( 3 )
Research paper	Reichhart et al., 2002, genesis 34(1-2): 160--164 Splice-activated UAS hairpin vector gives complete RNAi knockout of single or double target transcripts in Drosophila melanogaster. [FBrf0152370]
Personal communication to FlyBase	Reichhart, 2004.8, pJM1084. pJM1084. [FBrf0178906]
FlyBase analysis	FlyBase, 1996-, FlyBase inference based on genome sequence analysis. FlyBase inference based on genome sequence analysis. [FBrf0104946]