Recombinant construct P{J21.17-GAL4}

General Information
Symbol	P{J21.17-GAL4}	FlyBase ID	FBtp0016398
Feature type	transgenic_transposon

Size		Expression data	GAL4 reporter/driver
Associated insertions	1 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Sharma et al., 2002)
Uses	CV term Qualifiers & info Reference
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference
Component Alleles
Allele	Scer\GAL4^JO15
Reference(s)	(Sharma et al., 2002, Kamikouchi et al., 2006, Sarpal et al., 2003, Kamikouchi et al., 2009, Lee et al., 2010, Schoen et al., 2010, Yorozu et al., 2009)
Molecular data	Construct: A 5.8kb BamHI genomic fragment (which derives from the genome about 0.4kb from the H{Lw2} insertion site in the H{Lw2}J21.17 enhancer trap line) is fused upstream of a minimal promoter driving Scer\GAL4 expression. (Sharma et al., 2002)
Phenotypic class	auditory perception defective, with dia^{CA.Scer\UAS.T:Ivir\HA1} (Schoen et al., 2010) auditory perception defective, with Rcom\RA^Scer\UAS.cSa, Scer\GAL4^ph-p-NP1046 (Kamikouchi et al., 2009) locomotor behavior defective, with Ctet\TeTxLC^TNT.Scer\UAS (Kamikouchi et al., 2009) phototaxis defective, with Ctet\TeTxLC^TNT.Scer\UAS (Kamikouchi et al., 2009)
Phenotype manifest in
Other information
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS) Stage Tissue/Position (including subcellular localization) Reference pupal stage zone A Johnston organ neuron (Lee et al., 2010) zone B Johnston organ neuron (Lee et al., 2010) pharate adult stage P9 Johnston organ (Sarpal et al., 2003) Comment:reference states 60 hr APF Johnston organ neuron (Sarpal et al., 2003) Comment:reference states 60 hr APF sensory neuron \| subset (Sarpal et al., 2003) Comment:reference states 60 hr APF adult stage zone B Johnston organ neuron \| subset (Kamikouchi et al., 2006) zone A Johnston organ neuron \| subset (Kamikouchi et al., 2006) zone B Johnston organ neuron (Kamikouchi et al., 2009, Yorozu et al., 2009) zone A Johnston organ neuron (Kamikouchi et al., 2009, Yorozu et al., 2009) sound activated Johnston organ neuron (Kamikouchi et al., 2009, Yorozu et al., 2009)
Additional Information
Statement Reference Expression of Scer\GAL4[JO15] is seen in the ~50-100 neurons innervating AMMC zone A, and ~100-150 neurons innervating AMMC zone B. (Kamikouchi et al., 2009)

Marker for	zone B Johnston organ neuron (Lee et al., 2010) neuron <of> Johnston's organ (Sarpal et al., 2003) zone A Johnston organ neuron (Lee et al., 2010)
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 1 )
Bloomington	6753 w^*; P{J21.17-GAL4}JO15/TM3, Sb¹
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	Gal4^JO15 (Sarpal et al., 2003) JO15 (Kamikouchi et al., 2006, Kamikouchi et al., 2009, Lee et al., 2010, Schoen et al., 2010) JO-AB (Kamikouchi et al., 2009, Lee et al., 2010, Yorozu et al., 2009) P{J21.17-GAL4} (FlyBase, 1996-)
Secondary FlyBase IDs

References ( 7 )
Research paper	Lee et al., 2010, PLoS ONE 5(6): e11012 Drosophila TRPN(=NOMPC) channel localizes to the distal end of mechanosensory cilia. [FBrf0210994] Schoen et al., 2010, Proc. Natl. Acad. Sci. U.S.A. 107(30): 13396--13401 Increased activity of Diaphanous homolog 3 (DIAPH3)/diaphanous causes hearing defects in humans with auditory neuropathy and in Drosophila. [FBrf0211399] Kamikouchi et al., 2009, Nature 458(7235): 165--171 The neural basis of Drosophila gravity-sensing and hearing. [FBrf0207518] Yorozu et al., 2009, Nature 458(7235): 201--205 Distinct sensory representations of wind and near-field sound in the Drosophila brain. [FBrf0207559] Kamikouchi et al., 2006, J. Comp. Neurol. 499(3): 317--356 Comprehensive classification of the auditory sensory projections in the brain of the fruit fly Drosophila melanogaster. [FBrf0194812] Sarpal et al., 2003, Curr. Biol. 13(19): 1687--1696 Drosophila KAP interacts with the kinesin II motor subunit KLP64D to assemble chordotonal sensory cilia, but not sperm tails. [FBrf0167402] Sharma et al., 2002, genesis 34(1-2): 115--118 pPTGAL, a convenient Gal4 P-element vector for testing expression of enhancer fragments in Drosophila. [FBrf0152359]