Recombinant construct P{UAS-lacZ.IR}

General Information
Symbol	P{UAS-lacZ.IR}	FlyBase ID	FBtp0016505
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	0 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Presente et al., 2002, Kennerdell and Carthew, 2000)
Uses	CV term Qualifiers & info Reference
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference reporter gene (Presente et al., 2002) selectable marker (Kennerdell and Carthew, 2000)
Component Alleles
Allele	Ecol\lacZ^{dsRNA.IR.Scer\UAS}
Reference(s)	(Presente et al., 2002, Kuranaga et al., 2006, Kanuka et al., 2005, Kanuka et al., 2005, Presente et al., 2004, Ishida et al., 2012, Kennerdell and Carthew, 2000, Lee et al., 2009, Sekine et al., 2011, Pelisson et al., 2007)
Molecular data	Construct: Scer\UAS sequences drive expression of Ecol\lacZ dsRNA sequences. (Presente et al., 2004) Construct: Scer\UAS regulatory sequences drive expression of an RNA hairpin responder specific for Ecol\lacZ. (Presente et al., 2002) Scer\UAS regulatory sequences drive expression of an inverted repeat of Ecol\lacZ coding sequences (nucleotides 3090-3835 relative to GenBank accession J01636). One copy of the repeat used to generate the construct had an EcoRI site at the nucleotide 3090 end and a SfiI site (top strand sequence GGCCATCTTGGCC) at the nucleotide 3835 end. The second copy of the repeat used had a XhoI site at the nucleotide 3090 end and a different SfiI site (top strand sequence GGCCTAGATGGCC) at the nucleotide 3835 end. The resulting SfiI site created in the construct has a central ATCTA sequence that is nonpalindromic. Consequently, the inverted repeat has imperfect dyad symmetry. (Kennerdell and Carthew, 2000)
Phenotypic class
Phenotype manifest in
Other information

Allele	w^+mC
Reference(s)	(Kennerdell and Carthew, 2000)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	LacZ IR (Kuranaga et al., 2006, Ishida et al., 2012) P{UAS-lacZ.IR} (FlyBase, 1996-) UAS-IRlacZ (Presente et al., 2002, Kennerdell and Carthew, 2000) UAS-LacZi (Presente et al., 2004) UAS-lacZ IR (Kanuka et al., 2005) UAS-LacZ IR (Lee et al., 2009, Ishida et al., 2012) UAS-LacZ-IR (Sekine et al., 2011) UASt-IRlacZ (Pelisson et al., 2007)
Secondary FlyBase IDs

References ( 10 )
Research paper	Ishida et al., 2012, PLoS ONE 7(2): e30265 Prevention of Apoptosis by Mitochondrial Phosphatase PGAM5 in the Mushroom Body Is Crucial for Heat Shock Resistance in Drosophila melanogaster. [FBrf0217492] Sekine et al., 2011, J. Cell Sci. 124(17): 3006--3016 p38 MAPKs regulate the expression of genes in the dopamine synthesis pathway through phosphorylation of NR4A nuclear receptors. [FBrf0215011] Lee et al., 2009, Proc. Natl. Acad. Sci. U.S.A. 106(15): 6363--6368 Drosophila IKK-related kinase Ik2 and Katanin p60-like 1 regulate dendrite pruning of sensory neuron during metamorphosis. [FBrf0207681] Pelisson et al., 2007, J. Virol. 81(4): 1951--1960 A novel repeat-associated small interfering RNA-mediated silencing pathway downregulates complementary sense gypsy transcripts in somatic cells of the Drosophila ovary. [FBrf0195158] Kuranaga et al., 2006, Cell 126(3): 583--596 Drosophila IKK-related kinase regulates nonapoptotic function of caspases, via degradation of IAPs. [FBrf0194448] Kanuka et al., 2005, EMBO J. 24(21): 3793--3806 Drosophila caspase transduces Shaggy/GSK-3beta kinase activity in neural precursor development. [FBrf0190361] Presente et al., 2004, Proc. Natl. Acad. Sci. U.S.A. 101(6): 1764--1768 Notch is required for long-term memory in Drosophila. [FBrf0175078] Presente et al., 2002, genesis 34(1-2): 165--169 Transgene-mediated RNA interference defines a novel role for Notch in chemosensory startle behavior. [FBrf0152371] Kennerdell and Carthew, 2000, Nat. Biotechnol. 18(8): 896--898 Heritable gene silencing in Drosophila using double-stranded RNA. [FBrf0129894]
Supplementary material	Kanuka et al., 2005, EMBO J. 24(21): Drosophila caspase transduces Shaggy/GSK-3beta kinase activity in neural precursor development. [FBrf0191762]