Recombinant construct PBac{PB}

General Information
Symbol	PBac{PB}	FlyBase ID	FBtp0016553
Feature type	transgenic_transposon

Size	7.257	Expression data
Associated insertions	4822 available
Molecular map
Recent Updates
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FB2012_01
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Description & Uses
Species	synthetic construct
Location-dependent role	carries FRT site(s) (Thibault et al., 2004) deletion generation component (Thibault et al., 2004)
Description	CV term Qualifiers & info Reference transposon P-element (Cho et al., 2002, Kambris et al., 2003) Tni\piggyBac (Cho et al., 2002, Kambris et al., 2003, Thibault et al., 2004)
Uses	CV term Qualifiers & info Reference genomic insertion screen
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent AY515146
Segments & Size
Total Size	7.257
Left end	PBac 5\' (Thibault, 2003.12.19)
Right end	PBac 3\' (Thibault, 2003.12.19)

Segments	Number Orientation Symbol 1 + PBac_5'(5',Hp1) 2 + 001349 3 + Car1_frgmt.PB(H3,R1) 4 + 001069 5 + w^+mPB.FRT 7 + PBac_3'(Hp1,3')
Reference	(Thibault, 2003.12.19)
Features
	CV term Qualifiers & info Reference selectable marker (Kambris et al., 2003) selectable marker (Thibault et al., 2004)
Component Alleles
Allele	w^+mPB.FRT
Reference(s)	(Thibault et al., 2004)
Molecular data
Phenotypic class
Phenotype manifest in
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Assay mode
Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	pPBac{PB} (Thibault et al., 2004)
descendant(s)	PBac{PB3.WH3} (Meyer and Moussian, 2009)
Comments
	The w[+mPB.FRT] marker in the PBac{PB} element is flanked by 48bp Scer\FRT sites. Upon exposure to Scer\FLP1, these FRT sites efficiently recombine; the w[+mPB.FRT] marker is removed and a single 48bp FRT site is regenerated. The 48bp FRT site is significantly less efficient for longer distance recombination reactions than the longer FRTs commonly used in constructs designed for generation of chromosomal deficiencies. (Parks and Cook, 2010.6.3) report comment: Corresponds to coordinates 2404-9684 in GB:AY515146. (Thibault, 2003.12.19)
Stocks Listed in FlyBase ( 3767 )
Harvard	- PBac{PB}c00002 - PBac{PB}c00003 - PBac{PB}c00005 - PBac{PB}c00029 - PBac{PB}c00031 - PBac{PB}c00040 - PBac{PB}c00041 - PBac{PB}c00046 - PBac{PB}c00052 - PBac{PB}c00053 - PBac{PB}c00059 - PBac{PB}c00071
	More stocks available...
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	PBac{PB} (FlyBase, 1996-, Parks and Cook, 2010.6.3) PBac{w⁺.T} (FlyBase, 1996-) PBac{w^+mC.T} (Kambris et al., 2003) piggyBac (Cho et al., 2002) piggyBac_PB (Thibault, 2003.12.19) pPBac{ (FlyBase, 1996-)
Secondary FlyBase IDs

References ( 16 )
Research paper	Acehan et al., 2011, Biophys. J. 100(9): 2184--2192 Cardiolipin Affects the Supramolecular Organization of ATP Synthase in Mitochondria. [FBrf0213614] Kadam et al., 2009, Development 136(5): 739--747 FGF ligands in Drosophila have distinct activities required to support cell migration and differentiation. [FBrf0206790] Meyer and Moussian, 2009, Dev., Growth Diffn 51(5): 483--498 Drosophila multiplexin (Dmp) modulates motor axon pathfinding accuracy. [FBrf0208225] Baril and Therrien, 2006, Dev. Biol. 294(1): 232--245 Alphabet, a Ser/Thr phosphatase of the protein phosphatase 2C family, negatively regulates RAS/MAPK signaling in Drosophila. [FBrf0190087] Thompson et al., 2005, Dev. Cell 9(5): 711--720 Tumor suppressor properties of the ESCRT-II complex component Vps25 in Drosophila. [FBrf0190222] Thibault et al., 2004, Nature Genetics 36(3): 283--287 A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac. [FBrf0175002] Kambris et al., 2003, EMBO Rpts 4(1): 64--69 DmMyD88 controls dorsoventral patterning of the Drosophila embryo. [FBrf0155743] Cho et al., 2002, Cell 108(6): 865--876 Developmental control of blood cell migration by the Drosophila VEGF pathway. [FBrf0146914]
Personal communication to FlyBase	Parks and Cook, 2010.6.3, Information about the FRT sites present in the PBac{PB} element. Information about the FRT sites present in the PBac{PB} element. [FBrf0210944] Levis and Matthews, 2006.8.8, Sorting out Exelixis multiples. Sorting out Exelixis multiples. [FBrf0195873]
FlyBase analysis	FlyBase Curators, 2008, Insertion identifiers and alleles based on genomic location of insertions with respect to gene annotations. Insertion identifiers and alleles based on genomic location of insertions with respect to gene annotations. [FBrf0202955] FlyBase, 2007.6, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0199566] FlyBase, 1996-, FlyBase inference based on genome sequence analysis. FlyBase inference based on genome sequence analysis. [FBrf0104946] FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
DNA/RNA sequence record	Thibault, 2003.12.19, GenBank/EMBL/DDBJ: AY515146 Cloning vector piggyBac_PB, complete sequence. [FBrf0174224]
Computer file	Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection. Genomic mapping of Exelixis insertion collection. [FBrf0184340]