A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Recombinant construct PBac{PB}

General Information
Symbol PBac{PB} FlyBase ID FBtp0016553
Feature type transgenic_transposon
Size 7.257 Expression data
Associated insertions 4824 available
Molecular map
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Description
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FB2013_03
Alleles
Cdk8[c01804]
CalpB[4062]
Insertions
PBac{PB}CalpB[4062]
FB2013_02
All updates Click here to see a list of all updates to this record from FB2010_08 and on.
hide Description & Uses
Species synthetic construct
Location-dependent
role
carries FRT site(s)
(Thibault et al., 2004)
deletion generation component
(Thibault et al., 2004)
Description
CV term
Qualifiers & info
Reference
transposon
P-element
(Cho et al., 2002, Kambris et al., 2003)
Tni\piggyBac
(Cho et al., 2002, Kambris et al., 2003, Thibault et al., 2004)
Uses
CV term
Qualifiers & info
Reference
genomic insertion screen
Cloning Sites
Location
Restriction sites
Reference
hide Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ /
EMBL /
GenBank
DNA sequence
Extent
 
hide Segments & Size
Total Size
7.257
Left end
PBac 5\'
(Thibault, 2003.12.19)
Right end
PBac 3\'
(Thibault, 2003.12.19)
Segments
Number
Orientation
Symbol
1
+
PBac_5'(5',Hp1)
2
+
001349
3
+
Car1_frgmt.PB(H3,R1)
4
+
001069
5
+
w+mPB.FRT
7
+
PBac_3'(Hp1,3')
Reference (Thibault, 2003.12.19)
hide Features
CV term
Qualifiers & info
Reference
selectable
marker
(Kambris et al., 2003)
selectable
marker
(Thibault et al., 2004)
hide Component Alleles
Allele w+mPB.FRT
Reference(s) (Thibault et al., 2004)
Molecular data
Phenotypic class
Phenotype manifest in
Other information
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects expression of
hide Progenitors & Descendants
progenitor(s)
(Thibault et al., 2004)
descendant(s)
(Meyer and Moussian, 2009)
hide Comments
Additional information at: GDP Transposons http://flypush.imgen.bcm.tmc.edu/pscreen/transposons.html
(Bellen et al., 2011)
The w[+mPB.FRT] marker in the PBac{PB} element is flanked by 48bp Scer\FRT sites. Upon exposure to Scer\FLP1, these FRT sites efficiently recombine; the w[+mPB.FRT] marker is removed and a single 48bp FRT site is regenerated. The 48bp FRT site is significantly less efficient for longer distance recombination reactions than the longer FRTs commonly used in constructs designed for generation of chromosomal deficiencies.
(Parks and Cook, 2010.6.3)
report comment: Corresponds to coordinates 2404-9684 in GB:AY515146.
(Thibault, 2003.12.19)
hide Stocks Listed in FlyBase ( 3764 )
Harvard
-
PBac{PB}c00002
-
PBac{PB}c00003
-
PBac{PB}c00005
-
PBac{PB}c00029
-
PBac{PB}c00031
-
PBac{PB}c00040
-
PBac{PB}c00041
-
PBac{PB}c00046
-
PBac{PB}c00052
-
PBac{PB}c00053
-
PBac{PB}c00059
-
PBac{PB}c00071
hide External Crossreferences & Linkouts
Other Crossreferences
Linkouts
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
PBac{PB}
(FlyBase, 1996-, Parks and Cook, 2010.6.3)
PBac{w+.T}
(FlyBase, 1996-)
PBac{w+mC.T}
(Kambris et al., 2003)
piggyBac
(Cho et al., 2002)
piggyBac_PB
(Thibault, 2003.12.19)
pPBac{
(FlyBase, 1996-)
Secondary FlyBase IDs
hide References ( 18 )
Research paper
Acehan et al., 2011, Biophys. J. 100(9): 2184--2192
Cardiolipin Affects the Supramolecular Organization of ATP Synthase in Mitochondria. [FBrf0213614]
Bellen et al., 2011, Genetics 188(3): 731--743
The Drosophila gene disruption project: progress using transposons with distinctive site specificities. [FBrf0214229]
Pandey et al., 2011, PLoS ONE 6(11): e28106
The Glucuronyltransferase GlcAT-P Is Required for Stretch Growth of Peripheral Nerves in Drosophila. [FBrf0216828]
Reinecke et al., 2011, PLoS ONE 6(8): e23865
Implicating calpain in tau-mediated toxicity in vivo. [FBrf0214684]
Kadam et al., 2009, Development 136(5): 739--747
FGF ligands in Drosophila have distinct activities required to support cell migration and differentiation. [FBrf0206790]
Meyer and Moussian, 2009, Dev. Growth Differ. 51(5): 483--498
Drosophila multiplexin (Dmp) modulates motor axon pathfinding accuracy. [FBrf0208225]
Baril and Therrien, 2006, Dev. Biol. 294(1): 232--245
Alphabet, a Ser/Thr phosphatase of the protein phosphatase 2C family, negatively regulates RAS/MAPK signaling in Drosophila. [FBrf0190087]
Thompson et al., 2005, Dev. Cell 9(5): 711--720
Tumor suppressor properties of the ESCRT-II complex component Vps25 in Drosophila. [FBrf0190222]
Thibault et al., 2004, Nat. Genet. 36(3): 283--287
A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac. [FBrf0175002]
Kambris et al., 2003, EMBO Rep. 4(1): 64--69
DmMyD88 controls dorsoventral patterning of the Drosophila embryo. [FBrf0155743]
Cho et al., 2002, Cell 108(6): 865--876
Developmental control of blood cell migration by the Drosophila VEGF pathway. [FBrf0146914]
Personal communication to FlyBase
Parks and Cook, 2010.6.3, Information about the FRT sites present in the PBac{PB} element.
Information about the FRT sites present in the PBac{PB} element. [FBrf0210944]
Levis and Matthews, 2006.8.8, Sorting out Exelixis multiples.
Sorting out Exelixis multiples. [FBrf0195873]
Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection. (Computer file)
Genomic mapping of Exelixis insertion collection. (Computer file) [FBrf0184340]
FlyBase analysis
FlyBase Curators, 2008, Insertion identifiers and alleles based on genomic location of insertions with respect to gene annotations.
Insertion identifiers and alleles based on genomic location of insertions with respect to gene annotations. [FBrf0202955]
FlyBase, 2007.6, Assessment of transgenic construct insertion sites.
Assessment of transgenic construct insertion sites. [FBrf0199566]
FlyBase, 1992-, FlyBase curation.
FlyBase curation. [FBrf0105495]
DNA/RNA sequence record
Thibault, 2003.12.19, GenBank/EMBL/DDBJ: AY515146
Cloning vector piggyBac_PB, complete sequence. [FBrf0174224]