Recombinant construct PBac{3HPy⁺}

General Information
Symbol	PBac{3HPy⁺}	FlyBase ID	FBtp0016566
Feature type	transgenic_transposon

Size	8.198	Expression data
Associated insertions	110 available
Molecular map
Recent Updates
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FB2012_01
FB2011_10
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon Tni\piggyBac (Ring, 2001, Ring and Garza, 2003.6.10, Bellen et al., 2004)
Uses	CV term Qualifiers & info Reference analytical tool deletion generator (Ring, 2001) genomic insertion screen
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)	FlyBase-compiled
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size	8.198
Left end	PBac 5\' (Ring, 2001)
Right end	PBac 3\' (Ring, 2001)

Segments	Number Orientation Symbol 1 + PBac_5'(5',Hp1) 2 + 001356 3 - w⁺(6.1,r) 4 - 6.1_P3'(dl,P3') 5 - 6.1_Int3 6 + 001358 7 - y^+mDint25.2(S,S) 8 - 001357 9 + PBac_3'(Hp1,3')
Reference	(Ring, 2001)
Features
	CV term Qualifiers & info Reference selectable marker (Patton et al., 1992)
Component Alleles
Allele	y^+mDint2
Reference(s)	(Patton et al., 1992, Ring and Garza, 2003.6.10)
Molecular data	Construct: 5.2kb y⁺ gene including 2.8kb of 5' flanking sequences and 0.13kb of 3' sequences and lacks the tissue-specific bristle and tarsal claw enhancers. (Roseman et al., 1995) 5.2kb y gene fragment containing 2.8kb of 5' flanking and 0.13kb of 3' flanking DNA and lacking the intron that is the location of the tissue-specific bristle and tarsal claw enhancers. (Roseman et al., 1995) 5kb intronless SalI fragment containing the y gene. (Harrison and Perrimon, 1993) 5.2kb SalI y gene intronless fragment containing 2.9kb of 5' flanking and 0.3kb of 3' flanking DNA. (Patton et al., 1992)
Phenotypic class	body color defective (Ring and Garza, 2003.6.10, Roseman et al., 2001) visible (Roseman et al., 2001)
Phenotype manifest in	adult cuticle (Roseman et al., 2001) arista (Ring and Garza, 2003.6.10) macrochaeta (Ring and Garza, 2003.6.10) unguis (Ring and Garza, 2003.6.10)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Assay mode
Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments
	construct_comment: This is a 3\' half-P construct marked with y^+mDint. The yellow mini-gene and P 3\'element interrupt the piggyBac transposase gene. (Ring, 2001)
Stocks Listed in FlyBase ( 104 )
Bloomington	16296 y¹ w^*; PBac{3HPy⁺}C176 16260 y¹ w¹¹¹⁸; PBac{3HPy⁺}C028 16261 y¹ w¹¹¹⁸; PBac{3HPy⁺}C033 16264 y¹ w¹¹¹⁸; PBac{3HPy⁺}C040 16269 y¹ w¹¹¹⁸; PBac{3HPy⁺}C051 16272 y¹ w¹¹¹⁸; PBac{3HPy⁺}C065 16278 y¹ w¹¹¹⁸; PBac{3HPy⁺}C092 16299 y¹ w¹¹¹⁸; PBac{3HPy⁺}C198 16307 y¹ w¹¹¹⁸; PBac{3HPy⁺}C211 16319 y¹ w¹¹¹⁸; PBac{3HPy⁺}C255 16355 y¹ w¹¹¹⁸; PBac{3HPy⁺}C377 16273 y¹ w¹¹¹⁸; PBac{3HPy⁺}C066/CyO
	More stocks available...
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	PBac{3HPy⁺} (FlyBase, 1996-, Ring, 2001, Ring and Garza, 2003.6.10, Bellen et al., 2004)
Secondary FlyBase IDs

References ( 9 )
Research paper	Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Roseman et al., 2001, Genetics 158(1): 291--307 Long-range repression by multiple Polycomb Group (PcG) proteins targeted by fusion to a defined DNA-binding domain in Drosophila. [FBrf0135809] Roseman et al., 1995, Genetics 141(3): 1061--1074 A P element containing suppressor of Hairy-wing binding regions has novel properties for mutagenesis in Drosophila melanogaster. [FBrf0084323] Roseman et al., 1995, Development 121(11): 3573--3582 A Drosophila insulator protein facilitates dosage compensation of the X chromosome mini-white gene located at autosomal insertion sites. [FBrf0084324] Harrison and Perrimon, 1993, Curr. Biol. 3(7): 424--433 Simple and efficient generation of marked clones in Drosophila. [FBrf0057940] Patton et al., 1992, Nucleic Acids Res. 20(21): 5859--5860 Position-independent germline transformation in Drosophila using a cuticle pigmentation gene as a selectable marker. [FBrf0057421]
Personal communication to FlyBase	Ring and Garza, 2003.6.10, PBac Constructs from the Project 'Genome Wide Dispersal of Half-P Elements in Drosophila Using PiggyBac. PBac Constructs from the Project 'Genome Wide Dispersal of Half-P Elements in Drosophila Using PiggyBac. [FBrf0159884]
FlyBase analysis	FlyBase, 1996-, FlyBase inference based on genome sequence analysis. FlyBase inference based on genome sequence analysis. [FBrf0104946]
Thesis	Ring, 2001, Ph.D. Thesis, Florida State University, FL: xii + 219pp Functional analysis of the Drosophila genome using transposable elements. [FBrf0152434]

Recombinant construct PBac{3HPy+}

Recombinant construct PBac{3HPy⁺}