Recombinant construct PBac{5HPw⁺}

General Information
Symbol	PBac{5HPw⁺}	FlyBase ID	FBtp0016567
Feature type	transgenic_transposon

Size	6.938	Expression data
Associated insertions	239 available
Molecular map
Recent Updates
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FB2012_01
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon PBac (Ring, 2001) Tni\piggyBac (Ring and Garza, 2003.6.10, Bellen et al., 2004)
Uses	CV term Qualifiers & info Reference analytical tool deletion generator (Ring, 2001) genomic insertion screen
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)	FlyBase-compiled
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size	6.938
Left end	PBac 5\' (Ring, 2001)
Right end	PBac 3\' (Ring, 2001)

Segments	Number Orientation Symbol 1 + PBac_5'(5',B2) 2 - bamHI/bglII 3 + w⁺(r,6.1) 4 + 6.1_P5'(P5',r)0.261 5 + bamHI/bglII 6 + PBac(Bg2,Hp1) 7 + 001354 8 - w^+mC4.1(Sp) 9 + 001355 10 + PBac_3'(Hp1,3')
Reference	(Ring, 2001)
Features
	CV term Qualifiers & info Reference partial rescue (Pirrotta, 1988) selectable marker (Pirrotta, 1988)
Component Alleles
Allele	w^+mC
Reference(s)	(Pirrotta, 1988, Ring and Garza, 2003.6.10)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Assay mode
Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	PBac{Dmw} (Ring, 2001) pP{lacW} (Ring, 2001)
descendant(s)
Comments
	construct_comment: This is a 5\' half-P construct marked with w^+mC. The white mini-gene interrupts the piggyBac transposase open reading frame, but otherwise leaves the piggyBac element intact. (Ring, 2001)
Stocks Listed in FlyBase ( 231 )
Bloomington	16149 w¹¹¹⁸; PBac{5HPw⁺}B126 16023 y¹ w¹¹¹⁸ PBac{5HPw⁺}A056 16010 y¹ w¹¹¹⁸; PBac{5HPw⁺}A001 16020 y¹ w¹¹¹⁸; PBac{5HPw⁺}A044 16027 y¹ w¹¹¹⁸; PBac{5HPw⁺}A066 16036 y¹ w¹¹¹⁸; PBac{5HPw⁺}A086 16037 y¹ w¹¹¹⁸; PBac{5HPw⁺}A090 16038 y¹ w¹¹¹⁸; PBac{5HPw⁺}A091 16039 y¹ w¹¹¹⁸; PBac{5HPw⁺}A113 16041 y¹ w¹¹¹⁸; PBac{5HPw⁺}A119 16044 y¹ w¹¹¹⁸; PBac{5HPw⁺}A129 16047 y¹ w¹¹¹⁸; PBac{5HPw⁺}A133
	More stocks available...
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	PBac{5HPw⁺} (FlyBase, 1996-, Ring, 2001, Ring and Garza, 2003.6.10, Bellen et al., 2004)
Secondary FlyBase IDs

References ( 15 )
Research paper	Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Giordano et al., 2002, Genetics 160(2): 637--648 RNAi triggered by symmetrically transcribed transgenes in Drosophila melanogaster. [FBrf0144904] Lloyd et al., 2002, Genome 45(2): 296--312 Enhancer of garnet/δAP-3 is a cryptic allele of the white gene and identifies the intracellular transport system for the white protein. [FBrf0147069] Soldatov et al., 1999, Molec. Cell. Biol. 19(5): 3769--3778 TAFII40 protein is encoded by the e(y)1 gene: biological consequences of mutations. [FBrf0108279] Hagstrom et al., 1996, Genes Dev. 10(24): 3202--3215 Fab-7 functions as a chromatin domain boundary to ensure proper segment specification by the Drosophila bithorax complex. [FBrf0091065] Hing and Carlson, 1996, J. Neurobiol. 30(4): 454--464 Male-male courtship behavior induced by ectopic expression of the Drosophila white gene: Role of sensory function and age. [FBrf0089680] True et al., 1996, Genetics 142(3): 819--837 A genome-wide survey of hybrid incompatibility factors by the introgression of marked segments of Drosophila mauritiana chromosomes into Drosophila simulans. [FBrf0086664] Qian and Pirrotta, 1995, Genetics 139(2): 733--744 Dosage compensation of the Drosophila white gene requires both the X chromosome environment and multiple intragenic elements. [FBrf0080335] Dorer and Henikoff, 1994, Cell 77(7): 993--1002 Expansions of transgene repeats cause heterochromatin formation and gene silencing in Drosophila. [FBrf0072979] Chung et al., 1993, Cell 74(3): 505--514 A 5' element of the chicken -globin domain serves as an insulator in human erythroid cells and protects against position effect in Drosophila. [FBrf0064408] Roseman et al., 1993, EMBO J. 12(2): 435--442 The su(Hw) protein insulates expression of the Drosophila melanogaster white gene from chromosomal position-effects. [FBrf0058161]
Review	Pirrotta, 1988, Rodriguez, Denhardt, 1988: 437--456 Vectors for P-mediated transformation in Drosophila. [FBrf0048982]
Personal communication to FlyBase	Ring and Garza, 2003.6.10, PBac Constructs from the Project 'Genome Wide Dispersal of Half-P Elements in Drosophila Using PiggyBac. PBac Constructs from the Project 'Genome Wide Dispersal of Half-P Elements in Drosophila Using PiggyBac. [FBrf0159884]
FlyBase analysis	FlyBase, 1996-, FlyBase inference based on genome sequence analysis. FlyBase inference based on genome sequence analysis. [FBrf0104946]
Thesis	Ring, 2001, Ph.D. Thesis, Florida State University, FL: xii + 219pp Functional analysis of the Drosophila genome using transposable elements. [FBrf0152434]

Recombinant construct PBac{5HPw+}

Recombinant construct PBac{5HPw⁺}