Recombinant construct P{UAS-aPKC.ΔN}

General Information
Symbol	P{UAS-aPKC.ΔN}	FlyBase ID	FBtp0016734
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	0 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Betschinger et al., 2003)
Uses	CV term Qualifiers & info Reference binary system (targeted) aPKC (Betschinger et al., 2003) characterization aPKC (Betschinger et al., 2003)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference binary component (Betschinger et al., 2003)
Component Alleles
Allele	aPKC^ΔN.Scer\UAS
Reference(s)	(Langevin et al., 2005, Mayer et al., 2005, Betschinger et al., 2003, Lee et al., 2006, Djiane et al., 2005, Betschinger et al., 2006, Roegiers et al., 2005, Tian and Deng, 2008, Wirtz-Peitz et al., 2008, Zeng et al., 2010, Kaplan et al., 2011, Fletcher et al., 2012)
Molecular data	Construct: Scer\UAS drives expression of a truncated aPKC consisting of amino acids 180-606. (Betschinger et al., 2003)
Phenotypic class	lethal \| larval stage, with Scer\GAL4^{neur-GAL4-A101} (Roegiers et al., 2005) neuroanatomy defective, with Scer\GAL4^wor.PA (Lee et al., 2006) planar polarity defective, with Scer\GAL4^sev.EP (Djiane et al., 2005)
Phenotype manifest in	adult Malpighian tubule \| conditional \| somatic clone, with Scer\GAL4^{Scer\FRT.Act5C}, Scer\GAL80^αTub84B.PL (Zeng et al., 2010) eye photoreceptor cell, with Scer\GAL4^sev.EP (Djiane et al., 2005) larval brain & neuroblast \| supernumerary, with Scer\GAL4^wor.PA (Lee et al., 2006) larval brain \| posterior & neuroblast \| supernumerary (Betschinger et al., 2006) ommatidium, with Scer\GAL4^sev.EP (Djiane et al., 2005) tormogen cell \| somatic clone \| supernumerary, with Scer\GAL4^{neur-GAL4-A101} (Roegiers et al., 2005)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	aPKC.ΔN (Djiane et al., 2005) aPKC^DN (Kaplan et al., 2011) P{UAS-aPKC.ΔN} (FlyBase, 1996-) UAS:aPKC^ΔN (Tian and Deng, 2008) UAS.aPKCΔN (Fletcher et al., 2012) UAS>aPKC^ΔN (Wirtz-Peitz et al., 2008) UAS-aPKC.ΔN (Betschinger et al., 2006) UAS-aPKC^ΔN (Lee et al., 2006, Kaplan et al., 2011) UAS-aPKC-ΔN (Mayer et al., 2005) UAS-DaPKC^ΔN (Zeng et al., 2010) UAS-DaPKCΔN (Langevin et al., 2005)
Secondary FlyBase IDs

References ( 12 )
Research paper	Fletcher et al., 2012, Curr. Biol. 22(12): 1116--1122 Positive feedback and mutual antagonism combine to polarize crumbs in the Drosophila follicle cell epithelium. [FBrf0218646] Kaplan et al., 2011, PLoS ONE 6(4): e18616 Complex Interactions between GSK3 and aPKC in Drosophila Embryonic Epithelial Morphogenesis. [FBrf0213424] Zeng et al., 2010, J. Cell. Physiol. 224(3): 766--774 Tumor suppressors Sav/scrib and oncogene ras regulate stem-cell transformation in adult Drosophila malpighian tubules. [FBrf0211181] Tian and Deng, 2008, Development 135(3): 463--471 Lgl and its phosphorylation by aPKC regulate oocyte polarity formation in Drosophila. [FBrf0203052] Betschinger et al., 2006, Cell 124(6): 1241--1253 Asymmetric segregation of the tumor suppressor brat regulates self-renewal in Drosophila neural stem cells. [FBrf0189901] Lee et al., 2006, Nature 439(7076): 594--598 lgl, pins and aPKC regulate neuroblast self-renewal versus differentiation. [FBrf0191245] Djiane et al., 2005, Cell 121(4): 621--631 The apical determinants aPKC and dPatj regulate Frizzled-dependent planar cell polarity in the Drosophila eye. [FBrf0187224] Langevin et al., 2005, Curr. Biol. 15(10): 955--962 Lethal giant larvae controls the localization of notch-signaling regulators numb, neuralized, and Sanpodo in Drosophila sensory-organ precursor cells. [FBrf0187315] Mayer et al., 2005, Curr. Biol. 15(20): 1847--1854 Quantitative analysis of protein dynamics during asymmetric cell division. [FBrf0190032] Roegiers et al., 2005, Mol. Biol. Cell 16(8): 3480--3487 Regulation of membrane localization of Sanpodo by lethal giant larvae and neuralized in asymmetrically dividing cells of Drosophila sensory organs. [FBrf0188062] Betschinger et al., 2003, Nature 422(6929): 326--330 The Par complex directs asymmetric cell division by phosphorylating the cytoskeletal protein Lgl. [FBrf0157155]
Supplementary material	Wirtz-Peitz et al., 2008, Cell 135(1): Supplemental Data. [FBrf0211096]