Recombinant construct P{Gad1-GAL4.3.098}

General Information
Symbol	P{Gad1-GAL4.3.098}	FlyBase ID	FBtp0016769
Feature type	transgenic_transposon

Size		Expression data	GAL4 reporter/driver
Associated insertions	0 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Ng et al., 2002)
Uses	CV term Qualifiers & info Reference characterization Gad1 (Ng et al., 2002)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference selectable marker (Ng et al., 2002)
Component Alleles
Allele	Scer\GAL4^Gad1.3.098
Reference(s)	(Ng et al., 2002, Yu et al., 2004, Iyengar et al., 2006, Jefferis et al., 2004, Enell et al., 2010, Gao et al., 2008, Nässel et al., 2008, Hekmat-Scafe et al., 2010, Hamasaka et al., 2005, Chiang et al., 2011, Jansen et al., 2009, Kahsai and Winther, 2011)
Molecular data	Construct: 3.098kb of sequence from immediately upstream of the Gad1 putative translation start site drives expression of Scer\GAL4. (Ng et al., 2002)
Phenotypic class
Phenotype manifest in
Other information

Allele	w^+mC
Reference(s)	(Ng et al., 2002)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS) Stage Tissue/Position (including subcellular localization) Reference larval stage antennal lobe (Jefferis et al., 2004) GABAergic neuron (Nässel et al., 2008) prepupal stage P1 -- P4 organism (Jefferis et al., 2004) Comment:expression is absent adult stage intrinsic neuron \| subset (Ng et al., 2002, Yu et al., 2004) GABAergic neuron (Nässel et al., 2008) neuron \| subset of adult brain (Chen et al., 2012) eye (Chen et al., 2012) Comment:medium expression ellipsoid body (Chen et al., 2012) Comment:strong expression adult mushroom body (Chen et al., 2012) Comment:strong expression antennal lobe projection neuron (Chen et al., 2012) Comment:medium expression mushroom body dorsal paired medial cell (Chen et al., 2012) Comment:faint expression local interneuron of adult antennal lobe \| subset (Ng et al., 2002) adult antennal lobe glomerulus (Ng et al., 2002, Yu et al., 2004) local interneuron of adult antennal lobe (Yu et al., 2004) adult antennal lobe (Yu et al., 2004) adult antennal lobe \| restricted (Ng et al., 2002)
Additional Information
Statement Reference Expression is observed in GABAergic local interneurons which circumscribe the dorsal poles of the antennal lobes, and innervate all antennal glomeruli. (Ng et al., 2002) Expression is not observed in developing adult-specific antennal lobes during the early stages of pupal development. (Jefferis et al., 2004)

Marker for	local interneuron <of> antennal lobe (Yu et al., 2004) local interneuron <of> antennal lobe antennal glomerulus (Yu et al., 2004) antennal glomerulus (Ng et al., 2002, Yu et al., 2004) local interneuron <of> antennal lobe \| subset antennal glomerulus (Ng et al., 2002) larval antennal lobe (Jefferis et al., 2004) GABAergic neuron (Nässel et al., 2008) local interneuron <of> antennal lobe \| subset (Ng et al., 2002)
Reflects expression of	Gad1 (Ng et al., 2002, Jefferis et al., 2004, Yu et al., 2004)
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	GAD1-GAL4 (Jefferis et al., 2004, Hamasaka et al., 2005, Kahsai and Winther, 2011) GAD1-Gal4 (Gao et al., 2008) gad1-Gal4 (Nässel et al., 2008) Gad1-GAL4 (Hekmat-Scafe et al., 2010, Chiang et al., 2011, Kahsai and Winther, 2011) Gad1-Gal4 (Chiang et al., 2011, Enell et al., 2010) gad1-GAL4 (Jansen et al., 2009) Gad1 GAL4 (Kahsai and Winther, 2011) GAD-GAL4 (Yu et al., 2004) glutamic acid decarboxylase GAL4 (Kahsai and Winther, 2011) P{Gad1-GAL4.3.098} (FlyBase, 1996-) P{Gad1-Gal4} (Iyengar et al., 2006) P{w⁺;GAD1-GAL4} (Ng et al., 2002)
Secondary FlyBase IDs

References ( 12 )
Research paper	Chiang et al., 2011, Curr. Biol. 21(1): 1--11 Three-Dimensional Reconstruction of Brain-wide Wiring Networks in Drosophila at Single-Cell Resolution. [FBrf0212704] Kahsai and Winther, 2011, J. Comp. Neurol. 519(2): 290--315 Chemical neuroanatomy of the Drosophila central complex: distribution of multiple neuropeptides in relation to neurotransmitters. [FBrf0212649] Enell et al., 2010, PLoS ONE 5(12): e15780 Insulin Signaling, Lifespan and Stress Resistance Are Modulated by Metabotropic GABA Receptors on Insulin Producing Cells in the Brain of Drosophila. [FBrf0212696] Hekmat-Scafe et al., 2010, Genetics 184(1): 171--183 Seizure sensitivity is ameliorated by targeted expression of k+-cl- cotransporter function in the mushroom body of the Drosophila brain. [FBrf0209683] Jansen et al., 2009, J. Comp. Neurol. 517(3): 313--332 PICK1 expression in the Drosophila central nervous system primarily occurs in the neuroendocrine system. [FBrf0208848] Nässel et al., 2008, BMC Neurosci. 9: 90 A large population of diverse neurons in the Drosophila central nervous system expresses short neuropeptide F, suggesting multiple distributed peptide functions. [FBrf0206024] Iyengar et al., 2006, J. Comp. Neurol. 499(4): 583--602 Modular neuropile organization in the Drosophila larval brain facilitates identification and mapping of central neurons. [FBrf0192978] Hamasaka et al., 2005, J. Neurobiol. 65(3): 225--240 GABA modulates Drosophila circadian clock neurons via GABA(B) receptors and decreases in calcium. [FBrf0191024] Jefferis et al., 2004, Development 131(1): 117--130 Developmental origin of wiring specificity in the olfactory system of Drosophila. [FBrf0167525] Yu et al., 2004, Neuron 42(3): 437--449 Altered representation of the spatial code for odors after olfactory classical conditioning; memory trace formation by synaptic recruitment. [FBrf0175026] Ng et al., 2002, Neuron 36(3): 463--474 Transmission of olfactory information between three populations of neurons in the antennal lobe of the fly. [FBrf0152271]
Supplementary material	Gao et al., 2008, Neuron 60(2): Supplemental Experimental Procedures. [FBrf0210625]