Recombinant construct P{hs-GstD1::GstD2.D21-65-D1}

General Information
Symbol	P{hs-GstD1::GstD2.D21-65-D1}	FlyBase ID	FBtp0017344
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	0 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Akgul and Tu, 2002)
Uses	CV term Qualifiers & info Reference characterization GstD1 \| fusion construct (Akgul and Tu, 2002) GstD2 \| fusion construct (Akgul and Tu, 2002)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference chimeric protein (Akgul and Tu, 2002) molecular tag (Akgul and Tu, 2002) selectable marker (Akgul and Tu, 2002)
Component Alleles
Allele	GstD1::GstD2^{D21-65-D1.hs.T:Zzzz\FLAG}
Reference(s)	(Akgul and Tu, 2002)
Molecular data	Construct: A heat shock promoter drives expression of the 5' UTR of Hsp70, CCCCAAC of the 5' UTR of GstD1, the N-terminal 65 amino acids of GstD2 fused to the C-terminal 150 amino acids of GstD1 and tagged at the C-terminal end with T:Zzzz\FLAG and the 3' UTR of Act5C. (Akgul and Tu, 2002)
Phenotypic class
Phenotype manifest in
Other information

Allele	w^+mC
Reference(s)	(Akgul and Tu, 2002)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	D21-65-D1 (Akgul and Tu, 2002) P{hs-GstD1::GstD2.D21-65-D1} (FlyBase, 1996-)
Secondary FlyBase IDs

References ( 1 )
Research paper	Akgul and Tu, 2002, J. Biol. Chem. 277(38): 34700--34707 Evidence for a stabilizer element in the untranslated regions of Drosophila glutathione S-transferase D1 mRNA. [FBrf0152096]