Recombinant construct P{2PE-SU-scs-E3}

General Information
Symbol	P{2PE-SU-scs-E3}	FlyBase ID	FBtp0017362
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	0 available
Molecular map
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Majumder and Cai, 2003)
Uses	CV term Qualifiers & info Reference reporter construct twi (Majumder and Cai, 2003)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference regulatory/structural DNA (Majumder and Cai, 2003) reporter gene (Majumder and Cai, 2003)
Component Alleles
Allele	Ecol\lacZ^{twi.BR.scs.eve}
Reference(s)	(Majumder and Cai, 2003)
Molecular data	Construct: A 340bp gypsy insulator (SU) and a scs^0.9 insulator element is placed between a 560bp fragment containing two tandem copies of the twi proximal element (2PE) and the 500bp eve stripe 3 enhancer (E3). These regulatory sequences are placed upstream of a Ecol\lacZ gene. The order of sequences is 2PE - BR - scs - w. (Majumder and Cai, 2003)
Phenotypic class
Phenotype manifest in
Other information

Allele	gypsy\su(Hw)BR³⁴⁰
Reference(s)	(Majumder and Cai, 2003)
Molecular data	340bp gypsy insulator fragment containing 12 su(Hw) binding sites. (Cai and Levine, 1997) Construct: 340bp fragment from gypsy, carrying 12 su(Hw) binding sites. (Cai and Levine, 1995)
Phenotypic class
Phenotype manifest in
Other information

Allele	scs^0.9
Reference(s)	(Majumder and Cai, 2003)
Molecular data	Construct: 0.9kb fragment of scs. (Cai and Levine, 1995)
Phenotypic class
Phenotype manifest in
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{2PE-SU-scs-E3} (FlyBase, 1996-) PSC3 (Majumder and Cai, 2003)
Secondary FlyBase IDs

References ( 1 )
Research paper	Majumder and Cai, 2003, Proc. Natl. Acad. Sci. U.S.A. 100(9): 5223--5228 The functional analysis of insulator interactions in the Drosophila embryo. [FBrf0159300]