Recombinant construct P{Dmn-GFP.W}

General Information
Symbol	P{Dmn-GFP.W}	FlyBase ID	FBtp0017422
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	0 available
Molecular map
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Wojcik et al., 2001)
Uses	CV term Qualifiers & info Reference characterization Dmn (Wojcik et al., 2001) reporter construct vital \| Dmn (Wojcik et al., 2001)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference molecular tag (Wojcik et al., 2001) selectable marker (Wojcik et al., 2001)
Component Alleles
Allele	Dmn^T:Avic\GFP
Reference(s)	(Wojcik et al., 2001, Basto et al., 2004, Anderson et al., 2009, Li et al., 2004, Basto et al., 2004, Kracklauer et al., 2010, Sitaram et al., 2012)
Molecular data	Construct: Construct containing 2491bp of Dmn promoter/enhancer sequence, the Dmn transcribed region and 419bp 3' of the putative polyadenylation signal. The Dmn coding sequence is tagged at the C-terminal end with T:Avic\GFP. (Wojcik et al., 2001)
Phenotypic class	fertile (Wojcik et al., 2001) viable (Wojcik et al., 2001)
Phenotype manifest in
Other information

Allele	w^+mC
Reference(s)	(Wojcik et al., 2001)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	Dmn-GFP (Anderson et al., 2009) DMN-GFP (Sitaram et al., 2012) p50:GFP (Kracklauer et al., 2010) p50/Dmn-GFP (Wojcik et al., 2001) P50-GFP (Basto et al., 2004, Basto et al., 2004) P{Dmn-GFP.W} (FlyBase, 1996-)
Secondary FlyBase IDs

References ( 7 )
Research paper	Sitaram et al., 2012, Development 139(16): 2945--2954 Regulation of dynein localization and centrosome positioning by Lis-1 and asunder during Drosophila spermatogenesis. [FBrf0219060] Kracklauer et al., 2010, J. Cell Sci. 123(Pt 16): 2763--2772 The Drosophila SUN protein Spag4 cooperates with the coiled-coil protein Yuri Gagarin to maintain association of the basal body and spermatid nucleus. [FBrf0211482] Anderson et al., 2009, Mol. Biol. Cell 20(11): 2709--2721 Asunder is a critical regulator of dynein-dynactin localization during Drosophila spermatogenesis. [FBrf0208135] Basto et al., 2004, Curr. Biol. 14(1): 56--61 In Vivo Dynamics of the Rough Deal Checkpoint Protein during Drosophila Mitosis. [FBrf0167421] Li et al., 2004, Mol. Biol. Cell 15(7): 3005--3014 The Drosophila tctex-1 light chain is dispensable for essential cytoplasmic dynein functions but is required during spermatid differentiation. [FBrf0180499] Wojcik et al., 2001, Nat. Cell Biol. 3(11): 1001--1007 Kinetochore dynein: its dynamics and role in the transport of the Rough deal checkpoint protein. [FBrf0141673]
Supplementary material	Basto et al., 2004, Curr. Biol. 14(1): In vivo dynamics of the rough deal checkpoint protein during Drosophila mitosis. [FBrf0178908]