Recombinant construct PBac{GAL4D,EYFP}

General Information
Symbol	PBac{GAL4D,EYFP}	FlyBase ID	FBtp0017476
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	296 available
Molecular map
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2012_01
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Description & Uses
Species	synthetic construct
Location-dependent role	GAL4 driver/enhancer trap (Horn et al., 2003)
Description	CV term Qualifiers & info Reference transposon P-element (Hacker et al., 2003) Tni\piggyBac (Horn et al., 2003, Bellen et al., 2004)
Uses	CV term Qualifiers & info Reference enhancer trap binary system (Horn et al., 2003) genomic insertion screen
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference
Component Alleles
Allele	Avic\GFP^EYFP.3xP3
Reference(s)	(Horn et al., 2003, Forstemann et al., 2005, Hacker et al., 2003, Tamura et al., 2010)
Molecular data	Construct: The "3xP3" promoter sequence (an artificial promoter containing 3 Mmus\Pax6 homodimer binding sites) drives expression of the "EYFP" form ("yellow GFP") of Avic\GFP. (Horn and Wimmer, 2000)
Phenotypic class
Phenotype manifest in
Other information

Allele	Scer\GAL4^Δ.P\T
Reference(s)	(Horn et al., 2003, Hacker et al., 2003)
Molecular data	Construct: The minimal P\T promoter and first intron are fused upstream of an Scer\GAL4 deletion variant "II-9", which contains both the amino-terminal DNA-binding domain and the carboxy-terminal activation domain. This variant of Scer\GAL4 protein is almost as good an activator as the wild-type Scer\GAL4, but is supposed to be more stable. fs(1)K10 3' untranslated region sequences are also present. (Horn et al., 2003)
Phenotypic class
Phenotype manifest in
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Assay mode
Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	pPBac{GAL4D,EYFP} (Horn et al., 2003)
descendant(s)
Comments

Stocks Listed in FlyBase ( 246 )
Bloomington	19535 w^; PBac{GAL4D,EYFP}PL00529 cn¹ bw¹ 19361 w^; PBac{GAL4D,EYFP}PL00031 P{FRT(w^hs)}2A P{neoFRT}82B 19363 w^; PBac{GAL4D,EYFP}PL00038 P{FRT(w^hs)}2A P{neoFRT}82B 19371 w^; PBac{GAL4D,EYFP}PL00062 P{FRT(w^hs)}2A P{neoFRT}82B 19372 w^; PBac{GAL4D,EYFP}PL00067 P{FRT(w^hs)}2A P{neoFRT}82B 19374 w^; P{FRT(w^hs)}2A PBac{GAL4D,EYFP}PL00074 P{neoFRT}82B 19375 w^; PBac{GAL4D,EYFP}PL00077 P{FRT(w^hs)}2A P{neoFRT}82B 19379 w^; P{FRT(w^hs)}2A P{neoFRT}82B PBac{GAL4D,EYFP}PL00084 19384 w^; PBac{GAL4D,EYFP}PL00093 P{FRT(w^hs)}2A P{neoFRT}82B 19385 w^; PBac{GAL4D,EYFP}PL00097 P{FRT(w^hs)}2A P{neoFRT}82B 19386 w^; PBac{GAL4D,EYFP}PL00098 P{FRT(w^hs)}2A P{neoFRT}82B 19387 w^; P{FRT(w^hs)}2A P{neoFRT}82B PBac{GAL4D,EYFP}PL00102
	More stocks available...
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	{GAL4Δ} (Horn et al., 2003) Bac{3xP3-EYFP,p-GAL4Δ-K10} (Horn et al., 2003) pBAC{3xP3-EYFP,p-Gal4Δ-K10} (Forstemann et al., 2005) pBac{3xP3-EYFP,p-GAL4Δ-K10} (Hacker et al., 2003) PBac{GAL4D,EYFP} (FlyBase, 1996-, Bellen et al., 2004, Hacker et al., 2003)
Secondary FlyBase IDs
	FBtp0017775
References ( 10 )
Research paper	Tamura et al., 2010, J. Neurosci. 30(42): 14091--14101 Drosophila PQBP1 regulates learning acquisition at projection neurons in aversive olfactory conditioning. [FBrf0212135] Shpargel et al., 2009, Mol. Biol. Cell 20(1): 90--101 Gemin3 is an essential gene required for larval motor function and pupation in Drosophila. [FBrf0206864] Forstemann et al., 2005, PLoS Biol. 3(7): 1187--1201 Normal microRNA maturation and germ-line stem cell maintenance requires Loquacious, a double-stranded RNA-binding domain protein. [FBrf0188210] Padash Barmchi et al., 2005, J. Cell Biol. 168(4): 575--585 DRhoGEF2 regulates actin organization and contractility in the Drosophila blastoderm embryo. [FBrf0184065] Bellen et al., 2004, Genetics 167(2): 761--781 The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes. [FBrf0179132] Hacker et al., 2003, Proc. Natl. Acad. Sci. USA 100(13): 7720--7725 piggyBac-based insertional mutagenesis in the presence of stably integrated P elements in Drosophila. [FBrf0159706] Horn et al., 2003, Genetics 163(2): 647--661 piggyBac-based insertional mutagenesis and enhancer detection as a tool for functional insect genomics. [FBrf0158985] Horn and Wimmer, 2000, Dev. Genes Evol. 210(12): 630--637 A versatile vector set for animal transgenesis. [FBrf0134553]
FlyBase analysis	FlyBase, 2007.6, Assessment of transgenic construct insertion sites. Assessment of transgenic construct insertion sites. [FBrf0199566] FlyBase, 1996-, FlyBase inference based on genome sequence analysis. FlyBase inference based on genome sequence analysis. [FBrf0104946]