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General Information
Symbol
P{PTT-GB}
FlyBase ID
FBtp0017513
Feature type
transgenic_transposon
Component Allele(s)
Size
5.956
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Location-dependent role
Description
CV term
Qualifiers and info
Reference
Construct components
Product class / Tool use(s)
Encoded product / tool
Marker(s)
Description

The P{PTT-GB} construct contains an artificial exon that consists of a splice donor site, a multiple tag cassette (Tag:polyHis followed by EGFP without start and stop codons), and a splice acceptor site. The exon is designed to act as a protein trap element when inserted into a coding intron of an endogenous gene in the correct orientation and splice phase.

In addition to acting as a protein trap element, insertions of the P{PTT-GB} element may also act as enhancer trap lines. This can occur via the production of a mRNA in which the artificial EGFP exon is fused to sequences derived from the non-coding strand of the mini-w marker present at the 5' end of the construct (this sequence contains a cryptic start codon and is in a compatible frame with the EGFP coding sequence in P{PTT-GB}). Insertions that act as enhancer traps rather than protein traps are typically located upstream of the annotated start site of the trapped gene and the EGFP protein produced is localized to the nucleus, possibly due to fusion of the first exon of P\T gene sequence present in the construct to the EGFP coding sequence.

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Features
CV term
Qualifiers and info
Reference
molecular
tag
partial
rescue
selectable
marker
selectable
marker
Component Alleles
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
Comments
Stocks (41)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Secondary FlyBase IDs
    References (20)