Recombinant construct P{PTT-GA}

General Information
Symbol	P{PTT-GA}	FlyBase ID	FBtp0017514
Feature type	transgenic_transposon	Created / Updated	2003-08-22/2003-08-22

Size	5.956	Expression data
Location-dependent role		Associated insertions	115 available
Molecular map
Description & Uses
Species	synthetic construct
Description	CV term Qualifiers & info Reference transposon P-element (Morin et al., 2001, Buszczak et al., 2007)
Uses	CV term Qualifiers & info Reference genomic insertion screen protein trap gene trap vital (Morin and Chia, 2002.12.21)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)	FlyBase-compiled
Sequence
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size	5.956
Left end	P5\' (Morin and Chia, 2002.12.21)
Right end	P3\' (Morin and Chia, 2002.12.21)

Segments	Number Orientation Symbol 1 + CaSpeR_P5'(P5',Sp) 2 + EcoRI 3 + Mhc⁺0.100(SA) 4 + GA-EGFP 5 + Mhc⁺0.121(SD) 6 + 001360 7 + Car1_P3'(H3,P3')
Reference	(Morin and Chia, 2002.12.21)
Features
	CV term Qualifiers & info Reference selectable marker (Pirrotta, 1988) partial rescue (Pirrotta, 1988) protein trap (Morin and Chia, 2002.12.21) molecular tag (Morin et al., 2001) selectable marker (Buszczak et al., 2007)
Component Alleles
w^+mC
Reference(s)	(Pirrotta, 1988)
Molecular data	Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors. 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Minigene of the white cDNA. (Chung et al., 1993) Deletion of w^+mC 5' flanking sequences to position -305. (Qian and Pirrotta, 1995)
Phenotypic class	courtship defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^Sym.Scer\UAS, w^{a.Sym.Scer\UAS} (Giordano et al., 2002)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^Sym.Scer\UAS, w^{a.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Avic\GFP^{GA.T:Zzzz\His6}
Reference(s)	(Morin and Chia, 2002.12.21, Morin et al., 2001, Buszczak et al., 2007, Quinones-Coello et al., 2007)
Molecular data	Construct: GA splice variant of Avic\GFP, tagged with T:Zzzz\His6. (Morin and Chia, 2002.12.21)
Phenotypic class
Phenotype manifest in
Other information
w^+*
Reference(s)	(Buszczak et al., 2007)
Molecular data	Construct: Large intron is removed. (Jack et al., 1991)
Phenotypic class
Phenotype manifest in
Other information
Expression Data
Reporter Expression
Stage Tissue/Position Reference
Additional Information
Statement Reference

Assay mode
Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{PTT-GA} (FlyBase, 1996-, Quinones-Coello et al., 2007) P-GA (Morin et al., 2001) pPGA (Buszczak et al., 2007)
Secondary FlyBase IDs

References ( 17 )
Research paper	Buszczak et al., 2007, Genetics 175(3): 1505--1531 The Carnegie protein trap library: A versatile tool for Drosophila developmental studies. [FBrf0200345] Quinones-Coello et al., 2007, Genetics 175(3): 1089--1104 Exploring strategies for protein trapping in Drosophila. [FBrf0200349] Giordano et al., 2002, Genetics 160(2): 637--648 RNAi triggered by symmetrically transcribed transgenes in Drosophila melanogaster. [FBrf0144904] Lloyd et al., 2002, Genome 45(2): 296--312 Enhancer of garnet/δAP-3 is a cryptic allele of the white gene and identifies the intracellular transport system for the white protein. [FBrf0147069] Morin et al., 2001, Proc. Natl. Acad. Sci. USA 98(26): 15050--15055 A protein trap strategy to detect GFP-tagged proteins expressed from their endogenous loci in Drosophila. [FBrf0141725] Soldatov et al., 1999, Molec. Cell. Biol. 19(5): 3769--3778 TAFII40 protein is encoded by the e(y)1 gene: biological consequences of mutations. [FBrf0108279] Hagstrom et al., 1996, Genes Dev. 10(24): 3202--3215 Fab-7 functions as a chromatin domain boundary to ensure proper segment specification by the Drosophila bithorax complex. [FBrf0091065] Hing and Carlson, 1996, J. Neurobiol. 30(4): 454--464 Male-male courtship behavior induced by ectopic expression of the Drosophila white gene: Role of sensory function and age. [FBrf0089680] True et al., 1996, Genetics 142(3): 819--837 A genome-wide survey of hybrid incompatibility factors by the introgression of marked segments of Drosophila mauritiana chromosomes into Drosophila simulans. [FBrf0086664] Qian and Pirrotta, 1995, Genetics 139(2): 733--744 Dosage compensation of the Drosophila white gene requires both the X chromosome environment and multiple intragenic elements. [FBrf0080335] Dorer and Henikoff, 1994, Cell 77(7): 993--1002 Expansions of transgene repeats cause heterochromatin formation and gene silencing in Drosophila. [FBrf0072979] Chung et al., 1993, Cell 74(3): 505--514 A 5' element of the chicken -globin domain serves as an insulator in human erythroid cells and protects against position effect in Drosophila. [FBrf0064408] Roseman et al., 1993, EMBO J. 12(2): 435--442 The su(Hw) protein insulates expression of the Drosophila melanogaster white gene from chromosomal position-effects. [FBrf0058161] Jack et al., 1991, Europ. J. Biochem. 202(2): 441--446 Restriction enzymes permit quantitative determination of defined chromatin structures within the chromosome. [FBrf0053929]
Review	Pirrotta, 1988, Rodriguez, Denhardt, 1988: 437--456 Vectors for P-mediated transformation in Drosophila. [FBrf0048982]
Personal communication to FlyBase	Morin and Chia, 2002.12.21, Sequence of P{PPT-GA}, P{PPT-GB}, P{PPT-GC}. Sequence of P{PPT-GA}, P{PPT-GB}, P{PPT-GC}. [FBrf0157321]
FlyBase analysis	FlyBase, 1996-, FlyBase inference based on genome sequence analysis. FlyBase inference based on genome sequence analysis. [FBrf0104946]