Recombinant construct P{PTT-GA}

General Information
Symbol	P{PTT-GA}	FlyBase ID	FBtp0017514
Feature type	transgenic_transposon

Size	5.956	Expression data
Associated insertions	121 available
Molecular map
Recent Updates
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FB2013_03	Alleles Avic\GFP[trol-CA06698] Insertions P{PTT-GA}trol[CA06698]
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Description & Uses
Species	synthetic construct
Location-dependent role	GFP gene trap (Morin et al., 2001)
Description	CV term Qualifiers & info Reference transposon P-element (Morin et al., 2001, Buszczak et al., 2007)
Uses	CV term Qualifiers & info Reference gene trap vital (Morin and Chia, 2002.12.21) genomic insertion screen protein trap
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)	FlyBase-compiled
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size	5.956
Left end	P5\' (Morin and Chia, 2002.12.21)
Right end	P3\' (Morin and Chia, 2002.12.21)

Segments	Number Orientation Symbol 1 + CaSpeR_P5'(P5',Sp) 2 + EcoRI 3 + Mhc⁺0.100(SA) 4 + GA-EGFP 5 + Mhc⁺0.121(SD) 6 + 001360 7 + Car1_P3'(H3,P3')
Reference	(Morin and Chia, 2002.12.21)
Features
	CV term Qualifiers & info Reference molecular tag (Morin et al., 2001) partial rescue (Pirrotta, 1988) protein trap (Morin and Chia, 2002.12.21) selectable marker (Pirrotta, 1988) selectable marker (Buszczak et al., 2007)
Component Alleles
Allele	Avic\GFP^{GA.T:Zzzz\His6}
Reference(s)	(Morin and Chia, 2002.12.21, Morin et al., 2001, Buszczak et al., 2007, Quinones-Coello et al., 2007)
Molecular data	Construct: GA splice variant of Avic\GFP, tagged with T:Zzzz\His6. (Morin and Chia, 2002.12.21)
Phenotypic class
Phenotype manifest in
Other information

Allele	w^+*
Reference(s)	(Buszczak et al., 2007)
Molecular data	Construct: Large intron is removed. (Jack et al., 1991)
Phenotypic class
Phenotype manifest in
Other information

Allele	w^+mC
Reference(s)	(Pirrotta, 1988)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)	P{N473.SVS-A} (FlyProt project., 2009.11)
Comments

Stocks Listed in FlyBase ( 21 )
Bloomington	6844 w¹¹¹⁸ P{PTT-GA}Nrg^G00305 6842 w¹¹¹⁸; P{PTT-GA}clu^G00271
Kyoto	110624 P{PTT-GA}Pdi^G00198 110643 P{PTT-GA}clu^G00271 110658 P{PTT-GA}Nrg^G00305 110795 P{PTT-GA}Asph^ZCL1605 110802 P{PTT-GA}nrv2^ZCL1649 110956 P{PTT-GA}lost^ZCL3169 110958 P{PTT-GA}Pabp2^ZCL3178 110606 P{PTT-GA}Jupiter^G00147 110740 P{PTT-GA}Zasp66^ZCL0663 110763 P{PTT-GA}Jupiter^ZCL0931
	More stocks available...
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{PTT-GA} (FlyBase, 1996-, Quinones-Coello et al., 2007) P-GA (Morin et al., 2001) pGA (FlyProt project., 2009.11) pPGA (Buszczak et al., 2007)
Secondary FlyBase IDs

References ( 11 )
Research paper	Lerner et al., 2013, Dev. Cell 24(2): 159--168 A Rab10-Dependent Mechanism for Polarized Basement Membrane Secretion during Organ Morphogenesis. [FBrf0220718] Nezis et al., 2010, J. Cell Biol. 190(4): 523--531 Autophagic degradation of dBruce controls DNA fragmentation in nurse cells during late Drosophila melanogaster oogenesis. [FBrf0211647] Pertceva et al., 2010, Cell Biol. Int. 34(10): 991--996 The role of Drosophila hyperplastic discs gene in spermatogenesis. [FBrf0211767] Buszczak et al., 2009, Science 323(5911): 248--251 Drosophila stem cells share a common requirement for the histone H2B ubiquitin protease scrawny. [FBrf0207075] Reich et al., 2009, PLoS ONE 4(3): e4669 miRNA-dependent translational repression in the Drosophila ovary. [FBrf0207452] Buszczak et al., 2007, Genetics 175(3): 1505--1531 The Carnegie protein trap library: A versatile tool for Drosophila developmental studies. [FBrf0200345] Quinones-Coello et al., 2007, Genetics 175(3): 1089--1104 Exploring strategies for protein trapping in Drosophila. [FBrf0200349] Benoit et al., 2005, Dev. Cell 9(4): 511--522 An essential cytoplasmic function for the nuclear poly(A) binding protein, PABP2, in poly(A) tail length control and early development in Drosophila. [FBrf0190193] Morin et al., 2001, Proc. Natl. Acad. Sci. U.S.A. 98(26): 15050--15055 A protein trap strategy to detect GFP-tagged proteins expressed from their endogenous loci in Drosophila. [FBrf0141725]
Personal communication to FlyBase	FlyProt project., 2009.11, Notes on constructs used in generating CPTI protein trap lines. (Computer file) Notes on constructs used in generating CPTI protein trap lines. (Computer file) [FBrf0213656] Morin and Chia, 2002.12.21, Sequence of P{PPT-GA}, P{PPT-GB}, P{PPT-GC}. Sequence of P{PPT-GA}, P{PPT-GB}, P{PPT-GC}. [FBrf0157321]