Recombinant construct P{PTT-GA}
| General Information | |||
|---|---|---|---|
| Symbol | P{PTT-GA} | FlyBase ID | FBtp0017514 |
| Feature type | transgenic_transposon | Created / Updated | 2003-08-22/2003-08-22 |
| Size | 5.956 | Expression data | |
| Location-dependent role | Associated insertions | 115 available | |
| Molecular map | |||
Description & Uses
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| Species | synthetic construct | ||
| Description |
CV term
Qualifiers & info
Reference
transposon
P-element
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| Uses |
CV term
Qualifiers & info
Reference
genomic insertion screen
protein trap
gene trap
vital
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| Cloning Sites |
Location
Restriction sites
Reference
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Sequence Data
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| Sequence (FB) | FlyBase-compiled | ||
| Sequence | |||
| Associated Sequence Data | |||
| DDBJ
/
EMBL / GenBank |
DNA sequence
Extent
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Segments & Size
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| Total Size |
5.956
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| Left end |
P5\'
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| Right end |
P3\'
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| Segments |
Number
Orientation
Symbol
2
+
3
4
+
5
6
+
7
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| Reference | (Morin and Chia, 2002.12.21) | ||
Features
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CV term
Qualifiers & info
Reference
selectable
marker
partial
rescue
protein
trap
molecular
tag
selectable
marker
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Component Alleles
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w+mC |
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| Reference(s) | (Pirrotta, 1988) | ||
| Molecular data |
Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the
Casper series of vectors.
4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding
the transcription start site.
Minigene of the white cDNA.
Deletion of w+mC 5' flanking sequences to position -305.
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| Phenotypic class | |||
| Phenotype manifest in |
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| Other information | |||
Avic\GFPGA.T:Zzzz\His6 |
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| Reference(s) | (Morin and Chia, 2002.12.21, Morin et al., 2001, Buszczak et al., 2007, Quinones-Coello et al., 2007) | ||
| Molecular data |
Construct: GA splice variant of Avic\GFP, tagged with T:Zzzz\His6.
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| Phenotypic class | |||
| Phenotype manifest in | |||
| Other information | |||
w+* |
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| Reference(s) | (Buszczak et al., 2007) | ||
| Molecular data |
Construct: Large intron is removed.
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| Phenotypic class | |||
| Phenotype manifest in | |||
| Other information | |||
Expression Data
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| Reporter Expression | |||
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Stage
Tissue/Position
Reference
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| Additional Information | |||
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Statement
Reference
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| Assay mode | |||
| Marker for | |||
| Reflects expression of | |||
Progenitors & Descendants
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| progenitor(s) | |||
| descendant(s) | |||
Comments
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Synonyms & Secondary IDs
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| Reported As | |||
| Symbol Synonym |
P{PTT-GA}
P-GA
pPGA
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| Secondary FlyBase IDs | |||
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References
( 17 )
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| Research paper |
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| Review |
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| Personal communication to FlyBase |
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| FlyBase analysis |
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Description & Uses