Recombinant construct P{(FRT.Eye)(loxP.su(Hw)BR)Eye454bpYSW}

General Information
Symbol	P{(FRT.Eye)(loxP.su(Hw)BR)Eye454bpYSW}	FlyBase ID	FBtp0017519
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	0 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Golovnin et al., 2003)
Uses	CV term Qualifiers & info Reference characterization su(Hw)BR-y-AS-C (Golovnin et al., 2003) y (Golovnin et al., 2003) FRT construct y (Golovnin et al., 2003)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference regulatory/structural DNA (Golovnin et al., 2003)
Component Alleles
Allele	gypsy\su(Hw)BR⁴³⁰
Reference(s)	(Golovnin et al., 2003)
Molecular data	Contains 12 su(Hw)-binding sites, corresponding to bp 647-1077 in the gypsy transposon. (Kuhn et al., 2003) Consists of a 430bp gypsy\su(Hw)BR, gypsy sequences 647-1077 (coordinates of FBrf0045014). (Roseman et al., 1995) Consists of a 430bp gypsy\su(Hw)BR, including all 12 binding sites, nucleotides 647 to 1077 of FBrf0045014. (Roseman et al., 1993) Construct: Consists of a 430bp gypsy\su(Hw)BR, including all 12 binding sites. (Patton et al., 1992)
Phenotypic class
Phenotype manifest in
Other information

Allele	su(Hw)BR-y-AS-C^454bp
Reference(s)	(Golovnin et al., 2003)
Molecular data	Construct: A 454bp fragment from the intergenic region between y and ac, which contains su(Hw) protein binding sites. (Golovnin et al., 2003)
Phenotypic class
Phenotype manifest in
Other information

Allele	w^{Eye454bpYSW.Scer\FRT.P1\loxP}
Reference(s)	(Golovnin et al., 2003)
Molecular data	Construct: The y wing enhancer (located between -1863 to -2873bp relative to the y transcription start site) and the y body enhancer (located between -1266 and -1963bp relative to the y transcription start site) are fused upstream of w^+mC. An Scer\FRT cassette containing w regulatory sequences (from -1084 to -1465bp relative to the transcription start site) has been inserted between the y wing and body enhancers. The w regulatory sequences contain testis and eye enhancers. A 454bp potential insulator fragment (su(Hw)BR-y-AS-C^454bp) has been inserted downstream of the y body enhancer. A P1\loxP cassette containing the gypsy\su(Hw)BR⁴³⁰ insulator sequence has been inserted between the su(Hw)BR-y-AS-C^125bp sequence and the w sequences. (Golovnin et al., 2003)
Phenotypic class
Phenotype manifest in
Other information

Allele	y^{Eye454bpYSW.Scer\FRT.P1\loxP}
Reference(s)	(Golovnin et al., 2003)
Molecular data	Construct: Contains the entire y gene, including the wing enhancer (located between -1863 to -2873bp relative to the transcription start site) and the body enhancer (located between -1266 and -1963bp relative to the transcription start site). An Scer\FRT cassette containing w regulatory sequences (from -1084 to -1465bp relative to the transcription start site) has been inserted between the y wing and body enhancers. The w regulatory sequences contain testis and eye enhancers. A 454bp potential insulator fragment (su(Hw)BR-y-AS-C^454bp) has been inserted between the y body enhancer and y transcription start site (at position -893 relative to the transcription start site). A P1\loxP cassette containing the gypsy\su(Hw)BR⁴³⁰ insulator sequence has been inserted downstream of the y coding region. (Golovnin et al., 2003)
Phenotypic class	body color defective (Golovnin et al., 2003)
Phenotype manifest in	adult cuticle (Golovnin et al., 2003)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)	P{(FRT.Eye)(-loxP)Eye454bpYSW} (Golovnin et al., 2003) P{(-FRT)(-loxP)Eye454bpYSW} (Golovnin et al., 2003)
Comments

Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{(FRT.Eye)(loxP.su(Hw)BR)Eye454bpYSW} (FlyBase, 1996-)
Secondary FlyBase IDs

References ( 1 )
Research paper	Golovnin et al., 2003, Development 130(14): 3249--3258 An endogenous Su(Hw) insulator separates the yellow gene from the Achaete-scute gene complex in Drosophila. [FBrf0160557]