FlyBase Recombinant Construct Report: P{NPF-GAL4.1}

General Information

Symbol

P{NPF-GAL4.1}

FlyBase ID

FBtp0018000

Feature type

transgenic_transposable_element

Component Allele(s)

Scer\GAL4^NPF.1

Also Known As

npf-gal4, dNPF-Gal4, npfGal4

Size

Expression Data

GAL4 reporter/driver

Associated insertion(s)

2 available

Molecular map

Description and Uses

Construct components

Component allele Scer\GAL4^NPF.1

Transgenic product class

Component

Use(s)

Regulatory region(s)

NPF

NPF_npf-1kb

Encoded product / tool

GAL4

binary expression system - driver

Description

A 1kb fragment containing the NPF 5' regulatory region and part of the first exon regulates expression of a GAL4 driver.

(Wu et al., 2003)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

Scer\GAL4^NPF.1

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

larval NPF-DL1 cell

(Rohwedder et al., 2015)

larval NPF-DL2 cell

(Rohwedder et al., 2015)

larval NPF-SOG cell

(Rohwedder et al., 2015)

early third instar larval stage

peptidergic neuron | subset

(Wu et al., 2003)

larval protocerebrum | restricted

(Wu et al., 2003)

peptidergic neuron of protocerebrum

(Wu et al., 2003)

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

Additional Information

Statement

Reference

GAT13b. Alleles or genotypes used :P{UAS-GFP.U}

(FlyBase Curators, 2017, Wu et al., 2003)

ScerGAL4^NPF.1 drives expression in NPF neurons that potentially overlap with ple positive dopaminergic neurons in the dorsolateral, basolateral and ipsilateral dorsomedial parts of the embryonic/larval protocerebrum, and with Tdc2 positive tyraminergic/octopaminergic neurons in the embryonic/larval subesophageal ganglion.

(Rohwedder et al., 2015)

ScerGAL4^npf.1 drives expression in some of the clock neurons, including, in males, three LNd neurons, three or four l-LNv neurons (weaker signal) and the Pdf negative s-LNV neuron. In females, ScerGAL4^npf.1 drives expression in one less LNd and l-LNv neurons on average than males. Labelling is also detected in other cells in the central and lateral brain, including in the fan-shaped body. The expression pattern is similar to that of NPF protein, except that varicosities and axon terminals are less strongly stained with ScerGAL4^npf.1.

(Hermann et al., 2012)

ScerGAL4^npf.1 drives expression in neurons of the dorsal and lateral protocerebrum, fan-shaped body and ellipsoid body. The expression is more widespread than the one obtained with NPF, except that neurons in the subesophageal ganglion labelled by NPF are not labelled with ScerGAL4^npf.1.

(Krashes et al., 2009)

Expression of ScerGAL4^npf.1 is seen in three cells of the LNd neuronal cluster.

(Yoshii et al., 2008)

Expression in early third instar larvae is observed in four protocerebral neuropeptide F-positive neurons.

(Wu et al., 2003)

Marker for

adult NPF neuron

(Shao et al., 2017)

peptidergic neuron

(Wu et al., 2003)

Reflects expression of

NPF

(Feng et al., 2021, Shao et al., 2017, Hermann et al., 2012, Kahsai and Winther, 2011, Krashes et al., 2009, Yoshii et al., 2008)

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

descendant(s)

Comments

Stocks (2)

Stocks containing insertion(s) of this recombinant construct

Bloomington

25681

y¹ w^*; P{NPF-GAL4.1}2

25682

y¹ w^*; P{NPF-GAL4.1}1

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (19)

Reported As

Symbol Synonym

NPF-GAL4

(Miyamoto et al., 2024, Chen et al., 2023, Feng et al., 2021, Li et al., 2021, Sareen et al., 2021, Zhang et al., 2021, Hu et al., 2020, Williams et al., 2020, Corfas et al., 2019, Miyamoto and Amrein, 2019, Stern et al., 2019, Kim et al., 2017, Lee et al., 2017, Shao et al., 2017, Wu et al., 2013, Hergarden et al., 2012, Kahsai and Winther, 2011)

NPF-GAL4(2)

(Chung et al., 2017)

NPF-GAL4(3)

(Chung et al., 2017)

NPF-Gal4

(Brunßen and Suter, 2024, Chen et al., 2024, Landayan et al., 2021, Yu et al., 2021, Lee et al., 2020, Sapiro et al., 2019, Zhang et al., 2019, Ohhara et al., 2018, Liu et al., 2017, Wang et al., 2016, Albin et al., 2015, Shankar et al., 2015, Schoofs et al., 2014, Kaplan et al., 2008)

NPF-gal4

(Chng et al., 2017, Veenstra and Ida, 2014, Veenstra, 2009, Park et al., 2008, Veenstra et al., 2008, Yoshii et al., 2008)

NPFGal4

(Zer-Krispil et al., 2018)

Neuropeptide F GAL4

(Wu et al., 2019)

Npf GAL4

(Søvik et al., 2017)

Npf-GAL4

(Erion et al., 2016)

Npf-Gal4

(Okamoto and Nishimura, 2015, Marella et al., 2012)

P{NPF-GAL4.1}

(FlyBase, 1996-)

P{npf-GAL4.1}

(FlyBase, 1996-)

dNPF-GAL4

(Senapati et al., 2019, Krashes et al., 2009)

dNPF-Gal4

(Grabowska et al., 2020, Grabowska et al., 2018, Rohwedder et al., 2016, Rohwedder et al., 2015)

neuropeptide F-GAL4

(Kahsai and Winther, 2011)

npf-GAL4

(Pu et al., 2018, Ries et al., 2017, Shih et al., 2015, Tanenhaus et al., 2012, Chiang et al., 2011, Dierick and Greenspan, 2007)

npf-Gal4

(Poe et al., 2024, Liu et al., 2019, Beehler-Evans and Micchelli, 2015, Yuan et al., 2014, He et al., 2013, Chiang et al., 2011, Johard et al., 2009, Iyengar et al., 2006)

npf-gal4

(Beshel et al., 2017, Bahn et al., 2009, Chen et al., 2008, Lee et al., 2006, Wen et al., 2005, Wu et al., 2005, Wu et al., 2003)

npfGal4

(Hermann et al., 2012)

Secondary FlyBase IDs

References (74)

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