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General Information
Symbol
P{NPF-GAL4.1}
FlyBase ID
FBtp0018000
Feature type
transgenic_transposable_element
Component Allele(s)
Also Known As
npf-gal4, dNPF-Gal4, npfGal4
Size
Expression Data
GAL4 reporter/driver
Associated insertion(s)
Molecular map
Description and Uses
Construct components
Component allele Scer\GAL4NPF.1
Product class / Tool use(s)
Regulatory region(s)
Description

A 1kb fragment containing the NPF 5' regulatory region and part of the first exon regulates expression of a GAL4 driver.

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Component Alleles
Molecular data

A 1kb fragment containing the NPF 5' regulatory region and part of the first exon regulates expression of a GAL4 driver.

Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Information
Statement
Reference

GAT13b. Alleles or genotypes used :P{UAS-GFP.U}

Scer\GAL4NPF.1 drives expression in NPF neurons that potentially overlap with ple positive dopaminergic neurons in the dorsolateral, basolateral and ipsilateral dorsomedial parts of the embryonic/larval protocerebrum, and with Tdc2 positive tyraminergic/octopaminergic neurons in the embryonic/larval subesophageal ganglion.

Scer\GAL4NPF.1 drives expression in some of the clock neurons, including, in males, three LNd neurons, three or four l-LNv neurons (weaker signal) and the Pdf negative s-LNV neuron. In females, Scer\GAL4NPF.1 drives expression in one less LNd and l-LNv neurons on average than males. Labelling is also detected in other cells in the central and lateral brain, including in the fan-shaped body. The expression pattern is similar to that of NPF protein, except that varicosities and axon terminals are less strongly stained with Scer\GAL4NPF.1.

Scer\GAL4NPF.1 drives expression in neurons of the dorsal and lateral protocerebrum, fan-shaped body and ellipsoid body. The expression is more widespread than the one obtained with NPF, except that neurons in the subesophageal ganglion labelled by NPF are not labelled with Scer\GAL4NPF.1.

Expression of Scer\GAL4NPF.1 is seen in three cells of the LNd neuronal cluster.

Expression in early third instar larvae is observed in four protocerebral neuropeptide F-positive neurons.

 
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (2)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (19)
Reported As
Symbol Synonym
NPF-GAL4(2)
NPF-GAL4(3)
Neuropeptide F GAL4
P{NPF-GAL4.1}
P{npf-GAL4.1}
neuropeptide F-GAL4
Secondary FlyBase IDs
    References (65)