FlyBase Recombinant Construct Report: P{eve-GAL4.RN2}

General Information

Symbol

P{eve-GAL4.RN2}

FlyBase ID

FBtp0018002

Feature type

transgenic_transposable_element

Component Allele(s)

Scer\GAL4^eve.RN2

Also Known As

RN2-Gal4, RN2Gal4, eve-GAL4, P{RN2-GAL4}, HGN1-Gal4, eve'-GAL4^RN2, RN2-E-Gal4, Eve^RN2-GAL4

Size

Expression Data

GAL4 reporter/driver

Associated insertion(s)

2 available

Molecular map

Description and Uses

Construct components

Component allele Scer\GAL4^eve.RN2

Transgenic product class

Component

Use(s)

Regulatory region(s)

eve

Encoded product / tool

GAL4

binary expression system - driver

Description

Two tandem repeats of eve sequences from +7.9kb to +8.6kb (relative to the transcription start site) are fused upstream of eve promoter sequences from -275bp to +11bp followed by the GAL4 driver coding region. Sequence consisting of a 38bp multi-cloning sequence followed by eve DNA from +91 to +99 nucleotides and an ATG codon has been used to replace the GAL4 translation initiation signal with that of eve. eve 3' sequences from +1306bp to +1521bp are present downstream of the GAL4 coding region.

(Fujioka et al., 2003)

Two tandem repeats pof the RP2+a/pCC element from +7.9 (EcoRI) to +8.6 (NheI) is placed upstream of the eve 5' promoter. This controls expression of a GAL4 driver.

(Landgraf et al., 2003)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

Scer\GAL4^eve.RN2

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

pCC neuron

(Kuzin et al., 2005, Landgraf et al., 2003)

larval DA1 motor neuron

(Kuzin et al., 2005, Landgraf et al., 2003)

larval RP2 motor neuron

(Kuzin et al., 2005, Landgraf et al., 2003)

embryonic stage 16

larval RP2 motor neuron

Comment: reference states 13 hours of developmental time

(Bottenberg et al., 2009, Fujioka et al., 2003)

pCC neuron

(Fujioka et al., 2003)

larval DA1 motor neuron

Comment: reference states 13 hours of developmental time

(Bottenberg et al., 2009, Fujioka et al., 2003)

embryonic stage 17

dendrite of motor neuron

Comment: reference states 21 hours of developmental time

(Bottenberg et al., 2009)

Additional Information

Statement

Reference

Drives expression in a subset of motor neurons at all stages.

(FlyBase Curators, 2017)

ScerGAL4^eve.RN2 is expressed in a group of two to five neurons innervating the adult hindgut. These neurons innervate the circular muscles, and the epithelium underlying both the rectal valve and the inner muscle layers of the rectum.

(Cognigni et al., 2011)

Marker for

larval DA1 motor neuron

(Kuzin et al., 2005, Fujioka et al., 2003)

larval RP2 motor neuron

(Kuzin et al., 2005, Fujioka et al., 2003)

pCC neuron

(Kuzin et al., 2005, Fujioka et al., 2003)

Reflects expression of

eve

(Cognigni et al., 2011, Kuzin et al., 2005, Fujioka et al., 2003, Landgraf et al., 2003)

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

descendant(s)

Comments

construct_comment: The construction and transformation of P{RN2-GAL4} was described in Fujioka et al., 2003, Development 130: 5385--5400.

(Fujioka and Jaynes, 2003.12)

The construction and transformation of P{RN2-GAL4} was described in Fujioka et al., 2003, Development 130: 5385--5400.

(Fujioka and Jaynes, 2003.12)

Stocks (6)

Stocks containing insertion(s) of this recombinant construct

Bloomington

7473

w^*; P{eve-GAL4.RN2}P

7470

y¹ w^*; P{eve-GAL4.RN2}E

7471

w^*; P{eve-GAL4.RN2}E, P{UAS-tau-lacZ.B}3

7474

w^*; P{eve-GAL4.RN2}P, P{UAS-tau-lacZ.B}2

7475

w^*; P{eve-GAL4.RN2}P, P{UAS-mCD8::GFP.L}LL5

7472

y¹ w^*; P{eve-GAL4.RN2}E, P{UAS-mCD8::GFP.L}LL6

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (18)

Reported As

Symbol Synonym

Eve'-GAL4^RN2

(Kamiyama et al., 2015)

Eve^RN2-GAL4

(Ashley et al., 2019, Cheng et al., 2019)

HGN1-Gal4

(Zhang et al., 2014, Cognigni et al., 2011)

P{RN2-GAL4}

(Chu-Lagraff et al., 2010, Buescher et al., 2006, Fujioka and Jaynes, 2003.12, FlyBase, 1996-)

P{eve-GAL4.RN2}

(Schützler et al., 2019, FlyBase, 1996-)

RN-2-Gal4

(Daubert et al., 2010)

RN-GAL4

(Andrews et al., 2008)

RN2-E-Gal4

(He et al., 2020, Xu et al., 2016)

RN2-GAL4

(Wilhelm et al., 2022, Grintsevich et al., 2021, Manavalan et al., 2013, Zhou et al., 2012, Ping and Tsunoda, 2011, Hung et al., 2010, Lacin et al., 2009, Garces and Thor, 2006, Fujioka et al., 2005, Kuzin et al., 2005, Sanchez-Soriano and Prokop, 2005, Baines, 2004, Fujioka et al., 2003)

RN2-Gal4

(Nguyen et al., 2024, He et al., 2020, Kellermeyer et al., 2020, Newman et al., 2017, Clark et al., 2016, Xu et al., 2016, Wang et al., 2015, van den Brink et al., 2013, Chu-Lagraff et al., 2010, Li et al., 2010, Fradkin et al., 2008, Stone et al., 2008, Roy et al., 2007, Labrador, 2005, Labrador et al., 2005, Landgraf et al., 2003)

RN2-gal4

(Chu-Lagraff et al., 2010)

RN2Gal4

(Zarin et al., 2014, Zarin et al., 2012, Garg et al., 2007)

aCC/RP2-Gal4

(Chu-Lagraff et al., 2010)

eve'-GAL4^RN2

(Hsu et al., 2009)

eve-GAL4

(Banzai et al., 2022, Heinrich and Ryglewski, 2020, Schützler et al., 2019)

eve-Gal4^RN2

(Bottenberg et al., 2009)

eve-Gal4^RN

(Sanchez-Soriano et al., 2005)

eveRN2-GAL4

(Oswald et al., 2018)

Secondary FlyBase IDs

References (49)

Report Sections

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