Recombinant construct Herm{M.TTComp}

General Information
Symbol	Herm{M.TTComp}	FlyBase ID	FBtp0018169
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	0 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon Mdoa\Hermes (Lozovsky et al., 2002)
Uses	CV term Qualifiers & info Reference characterization Dmau\mariner\T (Lozovsky et al., 2002)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference
Component Alleles
Allele	Dmau\mariner\T^pch
Reference(s)	(Lozovsky et al., 2002)
Molecular data	There are 11 nucleotide differences between Dmau\mariner\T^pch and a functional mariner transposase; 3 changes upstream of the open reading frame, four missense and one silent substitution within the open reading frame, and 3 changes downstream of the open reading frame. (Medhora et al., 1991)
Phenotypic class
Phenotype manifest in
Other information

Allele	w^+mW.hs
Reference(s)	(Lozovsky et al., 2002)
Molecular data	A mini-w gene is expressed under the control of an Hsp70 promoter. (Heinrich et al., 1993) w gene expression is under the influence of FLIP recombinase. (Golic and Lindquist, 1989) Expression of a fragment of w genomic DNA with a 2.2kb deletion of 3' flanking sequences and a reduction in the size of the largest intron to 356bp is driven by a 388bp fragment of an Hsp70 gene. (Klemenz et al., 1987) Construct: A 'mini-white' gene constructed by deletion of the XbaI-HindII fragment from the long 5' intron. Carried by the W6 and W8 series of vectors.
Phenotypic class	suppressor of variegation, with Su(var)205⁵⁰² (Ahmad and Golic, 1996)
Phenotype manifest in	pigment cell (Kurkulos et al., 1994, Golic and Lindquist, 1989)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments
	construct_comment: Vector containing an almost complete duplication of M{peach} in tail-to-tail orientation. w^+mW.hs is inserted at the junction of the duplicated fragments. The innermost pair of inverted repeats (directly adjacent to the w^+mW.hs marker gene) are modified and are not susceptible to excision by Dmau\mariner\T, while the outermost inverted repeats are intact, and can, in principle, be used by Dmau\mariner\T to mobilise the vector. (Lozovsky et al., 2002) Vector containing an almost complete duplication of M{peach} in tail-to-tail orientation. w^+mW.hs is inserted at the junction of the duplicated fragments. The innermost pair of inverted repeats (directly adjacent to the w^+mW.hs marker gene) are modified and are not susceptible to excision by Dmau\mariner\T, while the outermost inverted repeats are intact, and can, in principle, be used by Dmau\mariner\T to mobilise the vector. (Lozovsky et al., 2002)
Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	Herm{M.TTComp} (FlyBase, 1996-) Herm{M{TTComp}} (FlyBase, 1996-) TTComp (Lozovsky et al., 2002)
Secondary FlyBase IDs

References ( 2 )
Research paper	Lozovsky et al., 2002, Genetics 160(2): 527--535 Unexpected Stability of mariner Transgenes in Drosophila. [FBrf0144896]
FlyBase analysis	FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]