FB2025_01 , released February 20, 2025
Recombinant Construct: P{UAS-synapto-pHluorin.nSyb.P}
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General Information
Symbol
P{UAS-synapto-pHluorin.nSyb.P}
FlyBase ID
FBtp0018352
Feature type
transgenic_transposable_element
Component Allele(s)
nSybP.UAS.pHluorinSE
Also Known As
UAS-n-Syb-pH, UAS-SpH
Size
Expression Data
Associated insertion(s)
0 available
Molecular map
Description and Uses
Construct components
Component allele nSybP.UAS.pHluorinSE
Transgenic product class
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
nSyb
Tagged with
pHluorinSE
Description

UASt regulatory sequences drive expression of the N-terminal of nSyb (amplified using PCR primers: 5' n-Syb (ACAGCCGAATTCGCTGAGGC) and 3' n-Syb-BsrB1 (CCAATTAGATCTCCGCTCACGCCGCCGTGATCGCC)) fused at its C-terminal to pHluorinSE (a pH-sensitive fluorescent protein).

(Poskanzer et al., 2003)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (6)
Reported As
Symbol Synonym
P{UAS-synapto-pHluorin.n-syb.P}
(FlyBase, 1996-)
P{UAS-synapto-pHluorin.nSyb.P}
(FlyBase, 1996-)
UAS-SpH
(Miśkiewicz et al., 2011, Kumar et al., 2009)
UAS-n-Syb-pH
(May et al., 2020, Dason et al., 2010, Poskanzer et al., 2003)
UAS-n-syb-pH
(Poskanzer et al., 2006)
synaptopHluorin
(Dason et al., 2010)
Secondary FlyBase IDs
    References (8)