Recombinant construct P{gypsy-lacZ.p12}

General Information
Symbol	P{gypsy-lacZ.p12}	FlyBase ID	FBtp0018658
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	3 available
Molecular map
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Sarot et al., 2004)
Uses	CV term Qualifiers & info Reference reporter construct gypsy element (Sarot et al., 2004)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference reporter gene (Sarot et al., 2004) selectable marker (Sarot et al., 2004)
Component Alleles
Allele	Ecol\lacZ^gypsy.p12
Reference(s)	(Sarot et al., 2004, Mevel-Ninio et al., 2007, Olivieri et al., 2010, Handler et al., 2011, Zamparini et al., 2011, Sienski et al., 2012)
Molecular data	Construct: The promoter and 5'UTR of gypsy (including the gypsy\su(Hw)BR) is fused upstream of Ecol\lacZ. (Sarot et al., 2004)
Phenotypic class
Phenotype manifest in
Other information

Allele	w^+mW.hs
Reference(s)	(Sarot et al., 2004)
Molecular data	A mini-w gene is expressed under the control of an Hsp70 promoter. (Heinrich et al., 1993) w gene expression is under the influence of FLIP recombinase. (Golic and Lindquist, 1989) Expression of a fragment of w genomic DNA with a 2.2kb deletion of 3' flanking sequences and a reduction in the size of the largest intron to 356bp is driven by a 388bp fragment of an Hsp70 gene. (Klemenz et al., 1987) Construct: A 'mini-white' gene constructed by deletion of the XbaI-HindII fragment from the long 5' intron. Carried by the W6 and W8 series of vectors.
Phenotypic class	suppressor of variegation, with Su(var)205⁵⁰² (Ahmad and Golic, 1996)
Phenotype manifest in	pigment cell (Kurkulos et al., 1994, Golic and Lindquist, 1989)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	gypsy-lacZ (Mevel-Ninio et al., 2007, Olivieri et al., 2010, Handler et al., 2011, Zamparini et al., 2011, Sienski et al., 2012) P{gypsy-lacZ.p12} (FlyBase, 1996-) p#12 (Sarot et al., 2004, Mevel-Ninio et al., 2007)
Secondary FlyBase IDs

References ( 6 )
Research paper	Sienski et al., 2012, Cell 151(5): 964--980 Transcriptional silencing of transposons by piwi and maelstrom and its impact on chromatin state and gene expression. [FBrf0220033] Handler et al., 2011, EMBO J. 30(19): 3977--3993 A systematic analysis of Drosophila TUDOR domain-containing proteins identifies Vreteno and the Tdrd12 family as essential primary piRNA pathway factors. [FBrf0216344] Zamparini et al., 2011, Development 138(18): 4039--4050 Vreteno, a gonad-specific protein, is essential for germline development and primary piRNA biogenesis in Drosophila. [FBrf0214783] Olivieri et al., 2010, EMBO J. 29(19): 3301--3317 An in vivo RNAi assay identifies major genetic and cellular requirements for primary piRNA biogenesis in Drosophila. [FBrf0212004] Mevel-Ninio et al., 2007, Genetics 175(4): 1615--1624 The flamenco locus controls the gypsy and ZAM retroviruses and is required for Drosophila oogenesis. [FBrf0200369] Sarot et al., 2004, Genetics 166(3): 1313--1321 Evidence for a piwi-dependent RNA silencing of the gypsy endogenous retrovirus by the Drosophila melanogaster flamenco gene. [FBrf0174688]