Recombinant construct P{GMR-Akt1.T342D.S505D}

General Information
Symbol	P{GMR-Akt1.T342D.S505D}	FlyBase ID	FBtp0018790
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	3 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Parks, 2004.5.12)
Uses	CV term Qualifiers & info Reference characterization Akt1 (Parks, 2004.5.12) GMR construct Akt1 (Parks, 2004.5.12)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference ectopic expression (Parks, 2004.5.12) selectable marker (Parks, 2004.5.12)
Component Alleles
Allele	Akt1^{T342D.S505D.GMR}
Reference(s)	(Parks, 2004.5.12, Kaplan et al., 2008, Wang et al., 2012)
Molecular data	Construct: Amino acid replacement: T342D. Amino acid replacement: S505D. (Parks, 2004.5.12)
Phenotypic class	visible (Kaplan et al., 2008)
Phenotype manifest in	eye (Kaplan et al., 2008) ommatidium (Kaplan et al., 2008)
Other information

Allele	w^+mC
Reference(s)	(Parks, 2004.5.12)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 2 )
Bloomington	8193 P{GMR-Akt1.T342D.S505D}1, y¹ w¹¹¹⁸ 8194 y¹ w¹¹¹⁸; P{GMR-Akt1.T342D.S505D}2
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	GMR-AKT^T342D,S505D (Wang et al., 2012) GMR-dAkt1^T342D-S505D (Kaplan et al., 2008) P{GMR-Akt1.T342D.S505D} (FlyBase, 1996-)
Secondary FlyBase IDs

References ( 3 )
Research paper	Wang et al., 2012, Mol. Cell. Biol. 32(12): 2203--2213 LST8 Regulates Cell Growth via Target-of-Rapamycin Complex 2 (TORC2). [FBrf0218417] Kaplan et al., 2008, Genes Dev. 22(14): 1877--1893 A nucleostemin family GTPase, NS3, acts in serotonergic neurons to regulate insulin signaling and control body size. [FBrf0205884]
Personal communication to FlyBase	Parks, 2004.5.12, Akt1 constructs and insertions. Akt1 constructs and insertions. [FBrf0178843]