FB2025_01 , released February 20, 2025
Recombinant Construct: PBac{RB}
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General Information
Symbol
PBac{RB}
FlyBase ID
FBtp0018839
Feature type
transgenic_transposable_element
Component Allele(s)
Rbp1SA.RB
Size
5.971
Expression Data
Associated insertion(s)
3938 available
Molecular map
Description and Uses
Description

The PBac{RB} element contains (from 5' to 3'): a w+mC marker, a single long (199bp) FRT site and a splice acceptor site (in the opposite orientation to the w+mC marker). The presence of the splice acceptor site in addition to the w+mC marker results in the element being mutagenic in both orientations.

(Thibault et al., 2004)

A diagram of the PBac{RB} element is available at the [GDP Transposons page](http://flypush.imgen.bcm.tmc.edu/pscreen/transposons.html)

(FlyBase, 1992-)
Construct components
Component
Use(s)
Element type
Also carries
FRT
Marker(s)
w+mC
Component allele Rbp1SA.RB
Transgenic product class
Component
Use(s)
Encoded product / tool
Rbp1
Description

Construct: An exon of Rbp1 has been engineered into the PBac{RB} transposon, in the opposite direction to the w+mC marker, to act as a splice trap.

(Thibault et al., 2004)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
AY515147
(bases )
Segments and Size
Total Size

5.971

Left end

PBac 5\'

(Thibault, 2003.12.19)
Right end

PBac 3\'

(Thibault, 2003.12.19)
 
Segments
Num
Orientation
Symbol
1
+
PBac_5'(5',B2)
2
+
001351
3
+
w+mC4.1(Sp)
4
+
001069
5
-
Car1_frgmt.WH
6
-
001343
7
-
FRT_200bp
8
+
001352
10
+
001353
11
+
PBac_3'(P1,3')
Reference
(Thibault, 2003.12.19)
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
Comments

report comment: Corresponds to coordinates 2404-8938 in GB:AY515147.

(Thibault, 2003.12.19)
Stocks (666)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
PBac{RB}
(Parks et al., 2004, Thibault et al., 2004, FlyBase, 1996-)
piggyBac_RB
(Thibault, 2003.12.19)
razorBac
(FlyBase, 1996-)
Secondary FlyBase IDs
    References (23)