General Information
Symbol
PBac{WH}
FlyBase ID
FBtp0018840
Feature type
transgenic_transposon
Component Allele(s)
Size
7.234
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Location-dependent role
carries FRT site(s)
deletion generation component
mobile activating element
Description
CV term
Qualifiers and info
Reference
transposon
Construct components
Product class / Tool use(s)
Encoded product / tool
Marker(s)
Description
The PBac{WH} element contains UAS regulatory sequences at its 5' end, oriented so that in the presence of a GAL4 driver, transcription from UAS is directed outwards from this end of the element. This can result in misexpression of neighbouring genomic loci when the element is inserted in the genome. In addition it contains a long (199bp) FRT site, a w+mC marker and a gypsy insulator sequence (at the 3' end of the element, downstream of the marker allele).
Component allele w+mC
Description
Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site.
Component allele TrlBRcRa
Description
Construct: Contains two copies of a 'GAGA element' which can bind Trl.
Component allele gypsy\su(Hw)BR348
Description
Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
(bases )
Segments and Size
Features
CV term
Qualifiers and info
Reference
mobile
activating
partial
rescue
regulatory/structural
DNA
selectable
marker
Component Alleles
Reference(s)
Molecular data
Allele
Reference(s)
Molecular data
Construct: Contains two copies of a 'GAGA element' which can bind Trl.
Allele
Reference(s)
Molecular data
4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site.
Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
Comments
report comment: Corresponds to coordinates 2404-9634 in GB:AY515148.
Additional information at: GDP Transposons http://flypush.imgen.bcm.tmc.edu/pscreen/transposons.html
Stocks (6,352)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Secondary FlyBase IDs
    References (37)