A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Recombinant construct PBac{WH}

General Information
Symbol PBac{WH} FlyBase ID FBtp0018840
Feature type transgenic_transposon
Size 7.234 Expression data
Associated insertions 6650 available
Molecular map
hide Recent Updates
Description
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This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
What does this section not display?
This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates Click here to see a list of all updates to this record from FB2010_08 and on.
hide Description & Uses
Species synthetic construct
Location-dependent
role
carries FRT site(s)
(Thibault et al., 2004)
deletion generation component
(Thibault et al., 2004)
mobile activating element
(Thibault et al., 2004)
Description
CV term
Qualifiers & info
Reference
transposon
P-element
(Parks et al., 2004)
Tni\piggyBac
(Thibault et al., 2004)
Uses
CV term
Qualifiers & info
Reference
analytical tool
carries FRT site(s)
(Thibault et al., 2004)
genomic insertion screen
mutagenesis
chromosomal aberrations
(Thibault et al., 2004)
Cloning Sites
Location
Restriction sites
Reference
hide Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ /
EMBL /
GenBank
DNA sequence
Extent
 
hide Segments & Size
Total Size
7.234
Left end
PBac 5\'
(Thibault, 2003.12.19)
Right end
PBac 3\'
(Thibault, 2003.12.19)
Segments
Number
Orientation
Symbol
1
+
PBac_5'(5',B2)
2
+
BglII
3
-
14xUASGhs.XP
4
+
001341
5
-
GAGA_sites
6
+
001342
7
+
FRT_200bp
8
+
001343
9
+
Car1_frgmt.WH
10
-
001069
11
-
w+mC4.1(Sp)
12
-
001068
13
+
001344
14
+
w+0.282
15
+
001345
16
-
su(Hw)BR.348
17
+
001347
18
+
PBac_3'(P1,3')
Reference (Thibault, 2003.12.19)
hide Features
CV term
Qualifiers & info
Reference
mobile
activating
(Thibault et al., 2004)
partial
rescue
(Pirrotta, 1988)
regulatory/structural
DNA
(Rorth, 1996)
selectable
marker
(Pirrotta, 1988)
hide Component Alleles
Allele gypsy\su(Hw)BR348
Reference(s) (Thibault et al., 2004)
Molecular data
Phenotypic class
Phenotype manifest in
Other information
Allele Scer\UAS14x.hs.XP.Scer\FRT
Reference(s) (Thibault et al., 2004)
Molecular data
Phenotypic class
Phenotype manifest in
Other information
Allele Scer\UAS14x.hs.XP
Reference(s) (Thibault et al., 2004)
Molecular data
Phenotypic class
Phenotype manifest in
Other information
Allele TrlBRcRa
Reference(s) (Rorth, 1996, Thibault et al., 2004)
Molecular data
Construct: Contains two copies of a 'GAGA element' which can bind Trl.
(Rorth, 1996)
Phenotypic class
Phenotype manifest in
Other information
Allele w+mC
Reference(s) (Pirrotta, 1988, Forstemann et al., 2005, Thibault et al., 2004, Lee et al., 2008)
Molecular data
Minigene of the white cDNA.
(Chung et al., 1993)
4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site.
(Pirrotta, 1988)
Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
 
Phenotypic class
courtship behavior defective | male
(Lloyd et al., 2002, Hing and Carlson, 1996)
eye color defective
(Soldatov et al., 1999, Hagstrom et al., 1996)
eye color defective, with Scer\GAL4Act5C.PI, wdsRNA.Sym.Scer\UAS
(Giordano et al., 2002)
visible | dominant
(True et al., 1996)
visible | recessive
(Roseman et al., 1993)
Phenotype manifest in
pigment cell
(Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995)
pigment cell, with Scer\GAL4Act5C.PI, wdsRNA.Sym.Scer\UAS
(Giordano et al., 2002)
Other information
hide Expression Data
Reporter Expression
Additional Information
Statement
Reference
Marker for
Reflects expression of
hide Progenitors & Descendants
progenitor(s)
(Thibault et al., 2004)
descendant(s)
(Parks et al., 2004)
(Parks et al., 2004)
(Meyer and Moussian, 2009)
(Parks et al., 2004)
(Parks et al., 2004)
(Parks et al., 2004)
hide Comments
Additional information at: GDP Transposons http://flypush.imgen.bcm.tmc.edu/pscreen/transposons.html
(Bellen et al., 2011)
report comment: Corresponds to coordinates 2404-9634 in GB:AY515148.
(Thibault, 2003.12.19)
hide Stocks Listed in FlyBase ( 6405 )
Harvard
-
PBac{WH}f00007
-
PBac{WH}f00008
-
PBac{WH}f00011
-
PBac{WH}f00012
-
PBac{WH}f00013
-
PBac{WH}f00015
-
PBac{WH}f00021
-
PBac{WH}f00022
-
PBac{WH}f00023
-
PBac{WH}f00025
-
PBac{WH}f00042
-
PBac{WH}f00043
hide External Crossreferences & Linkouts
Other Crossreferences
Linkouts
hide Synonyms & Secondary IDs
Reported As
Symbol Synonym
PBac{WH}
(FlyBase, 1996-, Thibault et al., 2004, Parks et al., 2004)
piggyBac_WH
(Thibault, 2003.12.19)
wartHog
(FlyBase, 1996-)
Secondary FlyBase IDs
hide References ( 28 )
Generate a list of
List References by type
hide Recent research papers ( 4 )
Chen and Ganetzky, 2012, J. Cell Biol. 196(4): 529--543
A neuropeptide signaling pathway regulates synaptic growth in Drosophila. [FBrf0217500]
Takaesu et al., 2012, Development 139(18): 3392--3401
Drosophila CORL is required for Smad2-mediated activation of Ecdysone Receptor expression in the mushroom body. [FBrf0219224]
Bellen et al., 2011, Genetics 188(3): 731--743
The Drosophila gene disruption project: progress using transposons with distinctive site specificities. [FBrf0214229]
Ye et al., 2011, J. Neurosci. 31(9): 3309--3319
Differential regulation of dendritic and axonal development by the novel kruppel-like factor dar1. [FBrf0213162]
Show all research papers ...