Recombinant construct PBac{RB3.WH3}

General Information
Symbol	PBac{RB3.WH3}	FlyBase ID	FBtp0018844
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	12 available
Molecular map
Recent Updates
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FB2013_03	Alleles LpR2[Df] Insertions PBac{RB3.WH3}LpR2[Df]
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Description & Uses
Species	synthetic construct
Location-dependent role	carries FRT site(s) (Parks et al., 2004) deletion-associated product (Parks et al., 2004)
Description	CV term Qualifiers & info Reference transposon Tni\piggyBac \| derivative (Parks et al., 2004)
Uses	CV term Qualifiers & info Reference mutagenesis chromosomal aberrations (Parks et al., 2004)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference
Component Alleles
Allele	w^+mC
Reference(s)	(Parks et al., 2004)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	PBac{RB} (Parks et al., 2004) PBac{WH} (Parks et al., 2004)
descendant(s)
Comments
	construct_comment: Product of recombination between Scer\FRT sites in progenitor transposons; results in a transgenic element with both termini derived from Tni\piggyBac 3\' end.Results in deletion or inversion of intervening genomic sequences.Contains single Scer\FRT site. (Parks et al., 2004) Product of recombination between Scer\FRT sites in progenitor transposons; results in a transgenic element with both termini derived from Tni\piggyBac 3\' end. (Parks et al., 2004) Results in deletion or inversion of intervening genomic sequences. (Parks et al., 2004) Contains single Scer\FRT site. (Parks et al., 2004)
Stocks Listed in FlyBase ( 6 )
Bloomington	38447 w¹¹¹⁸; PBac{RB3.WH3}CG7220¹/CyO 7805 w¹¹¹⁸; Df(2L)Exel9031, PBac{RB3.WH3}Exel9031 7951 w¹¹¹⁸; Df(3R)Exel9029, PBac{RB3.WH3}Exel9029 37744 w¹¹¹⁸; PBac{RB3.WH3}CG17360^M56/TM6B, Tb¹ 38449 w¹¹¹⁸; PBac{RB3.WH3}P5CDh1¹/TM6C, Sb¹ cu¹ 32331 y¹ w^*; Df(2R)Acsl^KO, PBac{RB3.WH3}Acsl^KO Acsl^KO/CyO, P{GAL4-Kr.C}DC3, P{UAS-GFP.S65T}DC7; +/TM6B, Tb¹
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	PBac{RB3.WH3} (FlyBase, 1996-, Parks et al., 2004)
Secondary FlyBase IDs

References ( 9 )
Research paper	Abbasi-Moheb et al., 2012, Am. J. Hum. Genet. 90(5): 847--855 Mutations in NSUN2 Cause Autosomal- Recessive Intellectual Disability. [FBrf0218249] Parra-Peralbo and Culi, 2011, PLoS Genet. 7(2): e1001297 Drosophila Lipophorin Receptors Mediate the Uptake of Neutral Lipids in Oocytes and Imaginal Disc Cells by an Endocytosis-Independent Mechanism. [FBrf0213038] Martin et al., 2009, Mol. Cell. Biol. 29(3): 861--870 A Drosophila pasha mutant distinguishes the canonical microRNA and mirtron pathways. [FBrf0207125] Parks et al., 2004, Nat. Genet. 36(3): 288--292 Systematic generation of high-resolution deletion coverage of the Drosophila melanogaster genome. [FBrf0175003]
Supplementary material	Frankel et al., 2010, Nature 466(7305): Online methods. [FBrf0212744]
Personal communication to FlyBase	Cash and Andrews, 2012.4.12, CG6330, P5CDh1, CG7609 & CG7220 intragenic deletions. CG6330, P5CDh1, CG7609 & CG7220 intragenic deletions. [FBrf0218082] Weiler, 2012.3.1, Df(3R)PS1, Df(3R)PS2, Df(3R)PS5 and CG17360[M56]. Df(3R)PS1, Df(3R)PS2, Df(3R)PS5 and CG17360[M56]. [FBrf0217887] Zhang and Wang, 2010.11.5, Acsl Df from Yi Zhang and Zhaohui Wang. Acsl Df from Yi Zhang and Zhaohui Wang. [FBrf0212314]
FlyBase analysis	FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]