Recombinant construct PBac{RB5.WH5}

General Information
Symbol	PBac{RB5.WH5}	FlyBase ID	FBtp0018845
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	11 available
Molecular map
Recent Updates
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FB2013_03	Alleles Lnk[Del29] Insertions PBac{RB5.WH5}Lnk[Del29]
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Description & Uses
Species	synthetic construct
Location-dependent role	carries FRT site(s) (Parks et al., 2004) deletion-associated product (Parks et al., 2004)
Description	CV term Qualifiers & info Reference transposon Tni\piggyBac \| derivative (Parks et al., 2004)
Uses	CV term Qualifiers & info Reference mobile activating element Scer\UAS (Parks et al., 2004) mutagenesis chromosomal aberrations (Parks et al., 2004)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference
Component Alleles
Allele	w^+mC
Reference(s)	(Parks et al., 2004)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	PBac{RB} (Parks et al., 2004) PBac{WH} (Parks et al., 2004)
descendant(s)
Comments
	construct_comment: Product of recombination between Scer\FRT sites in progenitor transposons; results in a transgenic element with both termini derived from Tni\piggyBac 5\' end.Results in deletion or inversion of intervening genomic sequences.Contains single Scer\FRT site. (Parks et al., 2004) Results in deletion or inversion of intervening genomic sequences. (Parks et al., 2004) Product of recombination between Scer\FRT sites in progenitor transposons; results in a transgenic element with both termini derived from Tni\piggyBac 5\' end. (Parks et al., 2004) Contains single Scer\FRT site. (Parks et al., 2004)
Stocks Listed in FlyBase ( 7 )
Bloomington	7885 w¹¹¹⁸; Df(2R)Exel9060, PBac{RB5.WH5}Exel9060 7925 w¹¹¹⁸; Df(3L)Exel9028, PBac{RB5.WH5}Exel9028 7946 w¹¹¹⁸; Df(3L)Exel9061, PBac{RB5.WH5}Exel9061 7818 w¹¹¹⁸; Df(2L)Exel9032, PBac{RB5.WH5}Exel9032/CyO 41798 w^*; Df(2R)hbs^j5, PBac{RB5.WH5}hbs^j5/CyO 7989 w¹¹¹⁸; Df(3R)Exel9030, PBac{RB5.WH5}Exel9030/TM6B, Tb¹ 38448 w¹¹¹⁸; PBac{RB5.WH5}CG7609¹/TM6C, Sb¹ cu¹
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	PBac{RB5.WH5} (FlyBase, 1996-, Parks et al., 2004)
Secondary FlyBase IDs

References ( 8 )
Research paper	Kim et al., 2012, Nature 483(7388): 209--212 The role of Drosophila Piezo in mechanical nociception. [FBrf0217681] Singh and Mlodzik, 2012, Dev. Cell 23(1): 82--96 Hibris, a Drosophila Nephrin Homolog, Is Required for Presenilin-Mediated Notch and APP-like Cleavages. [FBrf0218978] Slack et al., 2010, PLoS Genet. 6(3): e1000881 Regulation of lifespan, metabolism, and stress responses by the Drosophila SH2B protein, Lnk. [FBrf0210418] Moazzen et al., 2009, Mech. Dev. 126(8-9): 605--610 Non-requirement of a regulatory subunit of Protein Phosphatase 2A, PP2A-B', for activation of Sex comb reduced activity in Drosophila melanogaster. [FBrf0208658] Parks et al., 2004, Nat. Genet. 36(3): 288--292 Systematic generation of high-resolution deletion coverage of the Drosophila melanogaster genome. [FBrf0175003]
Supplementary material	Zhang et al., 2008, Dev. Cell 14(3): Supplemental Data. [FBrf0208874]
Personal communication to FlyBase	Cash and Andrews, 2012.4.12, CG6330, P5CDh1, CG7609 & CG7220 intragenic deletions. CG6330, P5CDh1, CG7609 & CG7220 intragenic deletions. [FBrf0218082]
FlyBase analysis	FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]