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General Information
Symbol
PBac{RB5.WH5}
FlyBase ID
FBtp0018845
Feature type
transgenic_transposable_element
Component Allele(s)
Size
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Description

The PBac{RB5.WH5} transgene represents a hybrid element produced by FLP-mediated recombination between an insertion of a PBac{RB} element and an insertion of a PBac{WH} element. PBac{RB5.WH5} consists of: terminus derived from the Tni\piggyBac 5' end - w+mC marker - single FRT site - UAS regulatory sequences (oriented so that in the presence of a GAL4 driver, transcription from UAS is directed outwards from the end of the element) - terminus derived from the Tni\piggyBac 5' end. PBac{RB5.WH5} is typically generated as part of a deletion-generation strategy; when the two insertions are in trans and are in the appropriate relative orientation, FLP-mediated recombination can result in deletion of the genomic region between the two original insertions along with production of the hybrid element at the recombination point. FLP-mediated recombination between a PBac{RB} and a PBac{WH} insertion can result in either a PBac{RB5.WH5} or a PBac{RB3.WH3} hybrid element, depending on the relative genomic locations and orientations of the original insertions.

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Sequence Data
Sequence (FB)
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DNA Sequence
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Additional Information
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Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
Comments
Stocks (12)
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Synonyms and Secondary IDs (1)
Reported As
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    References (17)