Recombinant construct P{Pdf-GAL80.S}

General Information
Symbol	P{Pdf-GAL80.S}	FlyBase ID	FBtp0019042
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	1 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Stoleru et al., 2004)
Uses	CV term Qualifiers & info Reference binary system (regulatory) Pdf (Stoleru et al., 2004)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference reporter gene (Stoleru et al., 2004)
Component Alleles
Allele	Scer\GAL80^Pdf.PS
Reference(s)	(Stoleru et al., 2004, Stoleru et al., 2007, Picot et al., 2007, Stoleru et al., 2005, Lim et al., 2007, Shafer et al., 2008, Keene et al., 2011, Hermann et al., 2012, Johard et al., 2009, Rieger et al., 2009, Helfrich-Forster et al., 2007, Lear et al., 2005, Parisky et al., 2008, Hodge and Stanewsky, 2008, Nagoshi et al., 2010, Lear et al., 2009, Chen et al., 2011, Sundram et al., 2012, Kaneko et al., 2012, Ruben et al., 2012)
Molecular data	Construct: The 2.4kb promoter drives expression of Pdf drives expression of Scer\GAL80. (Stoleru et al., 2004)
Phenotypic class	behavior defective, with Scer\GAL4^tim.PE, sgg^EP1576 (Stoleru et al., 2007) circadian behavior defective \| adult stage \| male \| heat sensitive, with Scer\GAL4^dimm-929, TrpA1^Scer\UAS.cKa (Parisky et al., 2008) circadian rhythm defective, with Scer\GAL4^tim.PE, sgg^EP1576 (Stoleru et al., 2005) circadian rhythm defective, with Scer\GAL4^tim.PE, Shaw^{RA.Scer\UAS.T:Zzzz\FLAG} (Hodge and Stanewsky, 2008) locomotor rhythm defective, with Scer\GAL4^cry.PE, W^Scer\UAS.cZa (Stoleru et al., 2004) locomotor rhythm defective, with Scer\GAL4^tim.PE, cry^{dsRNA.Scer\UAS.cPa} (Picot et al., 2007) locomotor rhythm defective, with Scer\GAL4^tim.PE, per^Scer\UAS.cKa (Stoleru et al., 2007) locomotor rhythm defective, with Scer\GAL4^tim.PE, per^Scer\UAS.cYa (Stoleru et al., 2007) locomotor rhythm defective, with Scer\GAL4^tim.PE, sgg^EP1576 (Stoleru et al., 2007) locomotor rhythm defective, with Scer\GAL4^tim.PE, Shaw^{RA.Scer\UAS.T:Zzzz\FLAG} (Hodge and Stanewsky, 2008) locomotor rhythm defective, with Scer\GAL4^tim.PE, tim^Scer\UAS.cYa (Stoleru et al., 2007) phototaxis defective, with Hsap\KCNJ2^{Scer\UAS.T:Avic\GFP-EGFP}, Scer\GAL4^tim.PE (Keene et al., 2011)
Phenotype manifest in
Other information
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS) Stage Tissue/Position (including subcellular localization) Reference adult stage LN Pdf neuron (Helfrich-Forster et al., 2007)
Additional Information
Statement Reference Scer\GAL80 expression in the LN Pdf neurons is inferred by loss of Avic\GFP[Scer\UAS.cUa] from Pdf neurons. (Helfrich-Forster et al., 2007)

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{Pdf-GAL80.S} (FlyBase, 1996-) Pdf-GAL80 (Stoleru et al., 2004, Hodge and Stanewsky, 2008, Nagoshi et al., 2010) pdfGal80 (Sundram et al., 2012, Hermann et al., 2012) Pdf-Gal80 (Chen et al., 2011, Ruben et al., 2012) PdfGAL80 (Stoleru et al., 2007, Lear et al., 2009) pdf-GAL80 (Lim et al., 2007, Shafer et al., 2008, Lear et al., 2005, Parisky et al., 2008) Pdf-gal80 (Rieger et al., 2009) pdf-Gal80 (Picot et al., 2007, Keene et al., 2011, Johard et al., 2009, Kaneko et al., 2012) pdf-gal80 (Helfrich-Forster et al., 2007)
Secondary FlyBase IDs

References ( 20 )
Research paper	Hermann et al., 2012, J. Comp. Neurol. 520(5): 970--987 Neuropeptide F immunoreactive clock neurons modify evening locomotor activity and free-running period in Drosophila melanogaster. [FBrf0217231] Kaneko et al., 2012, Curr. Biol. 22(19): 1851--1857 Circadian rhythm of temperature preference and its neural control in Drosophila. [FBrf0219631] Ruben et al., 2012, J. Biol. Rhythms 27(5): 353--364 A mechanism for circadian control of pacemaker neuron excitability. [FBrf0219602] Sundram et al., 2012, J. Biol. Rhythms 27(3): 183--195 Cellular Requirements for LARK in the Drosophila Circadian System. [FBrf0218481] Chen et al., 2011, Curr. Biol. 21(9): 719--729 QUASIMODO, a Novel GPI-Anchored Zona Pellucida Protein Involved in Light Input to the Drosophila Circadian Clock. [FBrf0213611] Keene et al., 2011, J. Neurosci. 31(17): 6527--6534 Distinct visual pathways mediate Drosophila larval light avoidance and circadian clock entrainment. [FBrf0213587] Nagoshi et al., 2010, Nat. Neurosci. 13(1): 60--68 Dissecting differential gene expression within the circadian neuronal circuit of Drosophila. [FBrf0209632] Johard et al., 2009, J. Comp. Neurol. 516(1): 59--73 Peptidergic clock neurons in Drosophila: ion transport peptide and short neuropeptide F in subsets of dorsal and ventral lateral neurons. [FBrf0208477] Lear et al., 2009, PLoS Biol. 7(7): e1000154 The neuropeptide PDF acts directly on evening pacemaker neurons to regulate multiple features of circadian behavior. [FBrf0208444] Rieger et al., 2009, J. Biol. Rhythms 24(4): 271--282 Period Gene Expression in Four Neurons Is Sufficient for Rhythmic Activity of Drosophila melanogaster under Dim Light Conditions. [FBrf0208345] Hodge and Stanewsky, 2008, PLoS ONE 3(5): e2274 Function of the Shaw potassium channel within the Drosophila circadian clock. [FBrf0210276] Parisky et al., 2008, Neuron 60(4): 672--682 PDF cells are a GABA-responsive wake-promoting component of the Drosophila sleep circuit. [FBrf0206340] Shafer et al., 2008, Neuron 58(2): 223--237 Widespread receptivity to neuropeptide PDF throughout the neuronal circadian clock network of Drosophila revealed by real-time cyclic AMP imaging. [FBrf0204718] Helfrich-Forster et al., 2007, J. Comp. Neurol. 500(1): 47--70 Development and morphology of the clock-gene-expressing lateral neurons of Drosophila melanogaster. [FBrf0193849] Lim et al., 2007, Curr. Biol. 17(12): 1082--1089 Clockwork orange encodes a transcriptional repressor important for circadian-clock amplitude in Drosophila. [FBrf0202276] Picot et al., 2007, PLoS Biol. 5(11): e315 Light activates output from evening neurons and inhibits output from morning neurons in the Drosophila circadian clock. [FBrf0202589] Stoleru et al., 2007, Cell 129(1): 207--219 The Drosophila circadian network is a seasonal timer. [FBrf0202062] Lear et al., 2005, Neuron 48(6): 965--976 The ion channel narrow abdomen is critical for neural output of the Drosophila circadian pacemaker. [FBrf0191323] Stoleru et al., 2005, Nature 438(7065): 238--242 A resetting signal between Drosophila pacemakers synchronizes morning and evening activity. [FBrf0191257] Stoleru et al., 2004, Nature 431(7010): 862--868 Coupled oscillators control morning and evening locomotor behaviour of Drosophila. [FBrf0179740]