FlyBase Recombinant Construct Report: P{Eaat1-GAL4.R}

FB2025_01 , released February 20, 2025

FB2025_01 , released February 20, 2025

Recombinant Construct: P{Eaat1-GAL4.R}

General Information

Symbol

P{Eaat1-GAL4.R}

FlyBase ID

FBtp0019053

Feature type

transgenic_transposable_element

Component Allele(s)

Scer\GAL4^Eaat1.PR

Also Known As

eaat1-Gal4, dEAAT1-GAL4, Eaat-Gal4

Size

Expression Data

GAL4 reporter/driver

Associated insertion(s)

Molecular map

Description and Uses

Construct components

Component allele Scer\GAL4^Eaat1.PR

Transgenic product class

Component

Use(s)

Regulatory region(s)

Encoded product / tool

binary expression system - driver

Description

An 8.5kb genomic segment that contains 4kb of the Eaat1 upstream region, the first exon and the first intron of Eaat1 fused to sequence encoding a GAL4 driver.

(Rival et al., 2004)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

Scer\GAL4^Eaat1.PR

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 11 -- 16

medial dorsal subperineurial glial cell

Comment: in 10-50% of examined hemisegments

(Beckervordersandforth et al., 2008)

Comment: in 10-50% of examined hemisegments

(Beckervordersandforth et al., 2008)

medial-most cell body glial cell

(Beckervordersandforth et al., 2008)

medial cell body glial cell

(Beckervordersandforth et al., 2008)

lateral cell body glial cell

(Beckervordersandforth et al., 2008)

first instar larval stage

larval ventral nerve cord | subset

• cell projection membrane

(Stacey et al., 2010)

embryonic longitudinal glial cell | subset

(Stacey et al., 2010)

Additional Information

Statement

Reference

Drives expression in predominantly in reticular neuropil associated glial cells (astrocytes), with some expression in cortex and ensheathing glial cells.

(FlyBase Curators, 2017)

ScerGAL4^Eaat1.PR drives expression in the same glial cells as EcollexA^GMR75H11, although slightly more in number.

(Aw et al., 2017)

ScerGAL4^Eaat1.PR labels neurons that have their cell bodies in the anterior and posterior of the medulla cortex. They send axons into the lamina neuropil via the optic chiasm, and some fibers are also seen in the medulla neuropil.

(Hamanaka and Meinertzhagen, 2010)

ScerGAL4^Eaat1.PR transcript is expressed in almost all of the the longitudinal (interface) glial cells where it is co-expressed AvicGFP^{CG31235.T:nls-tra}. The majority of the cells labeled with ScerGAL4^Eaat1.PR co-express pros, which marks the six anterior longitudinal glial cells. ScerGAL4^Eaat1.PR is expressed in the glial membrane projections in the ventral nerve cord, adjacent to VGlut1 puncta.

(Stacey et al., 2010)

ScerGAL4^Eaat1.PR drives expression in the glial processes that wrap the motor axons in the adult neuromuscular junction.

(Rival et al., 2006)

Marker for

Reflects expression of

(Hamanaka and Meinertzhagen, 2010, Stacey et al., 2010, Doherty et al., 2009, Beckervordersandforth et al., 2008, Rival et al., 2006, Lievens et al., 2005)

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

descendant(s)

Comments

Stocks (1)

Stocks containing insertion(s) of this recombinant construct

8849

w^*; P{Eaat1-GAL4.R}2; l(3)*^*/TM3, Sb¹

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (15)

Reported As

Symbol Synonym

EAAT1-GAL4

(Edwards et al., 2012)

EAAT1-Gal4

(Hess-Homeier et al., 2014)

EAAT1-gal4

(Sherer et al., 2020)

EEAT1-GAL4

(Hegde et al., 2014)

Eaat-Gal4

(Cusumano et al., 2019, Lee et al., 2018)

Eaat1-GAL4

(Haynes et al., 2024, Miao et al., 2024, Girard et al., 2020, Matsuno et al., 2019, Cho et al., 2018, Seugnet et al., 2011, Hamanaka and Meinertzhagen, 2010)

Eaat1-Gal4

(Petitgas et al., 2024, Li et al., 2023, You et al., 2021, Davla et al., 2020, Cusumano et al., 2019, Konstantinides et al., 2018, Aw et al., 2017, Peco et al., 2016, Stacey et al., 2010)

Eaat1G4

(Sengupta et al., 2019)

P{Eaat1-GAL4.R}

(Aw et al., 2017, FlyBase, 1996-)

dEAAT1-GAL4

(Dupont et al., 2012, Danjo et al., 2011, Besson et al., 2010, Sinakevitch et al., 2010, Liévens et al., 2008, Rival et al., 2006, Lievens et al., 2005, Rival et al., 2004, Rival et al., 2004)

dEAAT1-Gal4

(Scott et al., 2023, Richier et al., 2017, Nisoli et al., 2010, Beckervordersandforth et al., 2008)

dEaat1-GAL4

(Ren et al., 2018, Yeh et al., 2018)

dEaat1Gal4

(Ng et al., 2011)

eaat1-GAL4

(Sengupta et al., 2019)

eaat1-Gal4

(Doherty et al., 2009)

Secondary FlyBase IDs

References (40)

Report Sections