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Recombinant construct P{ple-GAL4.F}
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| Symbol | P{ple-GAL4.F} | FlyBase ID | FBtp0020119 |
| Feature type | transgenic_transposon | ||
| Size | Expression data | GAL4 reporter/driver | |
| Associated insertions | 1 available | ||
| Molecular map | |||
Recent Updates
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| FB2013_03 | |||
| FB2013_02 |
Controlled Vocabulary Terms
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| All updates | Click here to see a list of all updates to this record from FB2010_08 and on. | ||
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Description & Uses
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| Species | synthetic construct | ||
| Location-dependent
role |
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| Description |
CV term
Qualifiers & info
Reference
transposon
P-element
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| Uses |
CV term
Qualifiers & info
Reference
binary system (regulatory)
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| Cloning Sites |
Location
Restriction sites
Reference
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Sequence Data
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| Sequence (FB) | |||
| Associated Sequence Data | |||
| DDBJ
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EMBL / GenBank |
DNA sequence
Extent
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Segments & Size
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| Total Size | |||
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| Right end | |||
| Segments |
Number
Orientation
Symbol
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Features
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CV term
Qualifiers & info
Reference
reporter
gene
selectable
marker
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Component Alleles
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| Allele | Scer\GAL4ple.PF | ||
| Reference(s) | (Zhang et al., 2007, Liu et al., 2007, Menzies et al., 2005, Andretic et al., 2005, Friggi-Grelin et al., 2003, Lee et al., 2008, Riemensperger et al., 2005, Yang et al., 2005, Sykes and Condron, 2005, Whitworth et al., 2005, Auluck et al., 2005, Yang, 2005, Park et al., 2006, Schwaerzel et al., 2003, Lee et al., 2008, Lima and Miesenbock, 2005, Park et al., 2006, Yuan et al., 2006, Wheeler et al., 2006, Schroll et al., 2006, Yang et al., 2008, Ganguly-Fitzgerald et al., 2006, Melcher and Pankratz, 2005, Cooper et al., 2006, Muñoz-Soriano and Paricio, 2007, Stavropoulos and Young, 2011, Lavara-Culebras and Paricio, 2007, Ishida et al., 2012, Wang et al., 2011, Chen et al., 2012, Kim et al., 2012, Gonzalo-Gomez et al., 2012, Draper et al., 2007, Imai et al., 2008, Kaplan et al., 2008, Gruenewald et al., 2009, Palgi et al., 2009, Park et al., 2009, Corl et al., 2009, Yang et al., 2008, Yang et al., 2006, Al-Anzi et al., 2009, Honjo and Furukubo-Tokunaga, 2009, Sakai et al., 2009, Gao et al., 2008, Sitaraman et al., 2008, Vömel and Wegener, 2008, Wu et al., 2010, Venderova et al., 2009, Tanaka et al., 2008, Lee et al., 2007, Alekseyenko et al., 2010, Nässel et al., 2008, Lee et al., 2008, Melicharek et al., 2010, Schroeder et al., 2003, Makos et al., 2010, Kim et al., 2010, de Haro et al., 2010, Yu et al., 2010, Imai et al., 2010, Du et al., 2010, White et al., 2010, Mao and Davis, 2009, Yarali and Gerber, 2010, Chiang et al., 2011, Friggi-Grelin et al., 2003, Riemensperger et al., 2011, Bayersdorfer et al., 2010, Gehrke et al., 2010, Rival et al., 2011, Bang et al., 2011, Giang et al., 2011, Vargas et al., 2011, Lee et al., 2011, Wakabayashi-Ito et al., 2011, Inagaki et al., 2012, Jepson et al., 2011, Claridge-Chang et al., 2009, Aso et al., 2012, Neckameyer and Bhatt, 2012, Jansen et al., 2009, Trinh et al., 2008, Kong et al., 2010, Barone et al., 2011, Freeman et al., 2012, Xiong et al., 2012, Chen et al., 2012, Pauls et al., 2010, Zhang et al., 2007, Pauls et al., 2010, Dierick and Greenspan, 2007, Kallijärvi et al., 2012, Munoz-Soriano et al., 2012, Kaun et al., 2011, Liu et al., 2012, Schneider et al., 2012, Kahsai et al., 2012, Keleman et al., 2012, Duran et al., 2012) | ||
| Molecular data |
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| Phenotypic class |
lethal, with EgfrDN.Scer\UAS
short lived, with kermitGD7327
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| Phenotype manifest in |
dopaminergic neuron & adult brain, with Sep4Scer\UAS.cMa
mitochondrion & dopaminergic neuron, with MarfScer\UAS.T:Zzzz\FLAG
mitochondrion & dopaminergic neuron, with opa1-likeScer\UAS.T:Zzzz\FLAG
mitochondrion & dorso-lateral dopaminergic neuron, with parkScer\UAS.T:Ivir\HA1
mitochondrion & dorso-lateral dopaminergic neuron, with Pink1dsRNA.Scer\UAS
mitochondrion & dorso-lateral dopaminergic neuron, with Pink1Scer\UAS.cYa
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| Other information | |||
| Allele | w+mC | ||
| Reference(s) | (Friggi-Grelin et al., 2003) | ||
| Molecular data |
Minigene of the white cDNA.
4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding
the transcription start site.
Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the
Casper series of vectors.
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| Phenotypic class | |||
| Phenotype manifest in |
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| Other information | |||
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Expression Data
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| Reporter Expression | |||
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distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
Comment:medium expression
Comment:faint expression
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| Additional Information | |||
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Statement
Reference
Scer\GAL4[ple.PF] generally labels all dopaminergic neurons.
Scer\GAL4[ple.PF] drives expression in the dopaminergic PPL1 neurons (includes MB-MP1, MB-V1 and MB-MV1 neurons), PPL2ab, in 118 PAM neurons
per hemisphere (includes MB-M3 neurons), in PPM3, in 1 PPM1 neuron per hemisphere, in PPM2, PPL2c, VUM1 and VUM2 neurons.
Expression of Scer\GAL4[ple.PF] is also detected in dopaminergic T1, SVP1, D1 and SP1 neurons.
Scer\GAL4[ple.PF] is expressed in approximately 12 PPL1, 11 PPL2, 8 PPM1/2, 7 PPM3 and 5 PAL dopaminergic neurons, but is not expressed well
in the PAM cluster. PAL neurons are seen to project across the midline. Neurons of the PPM1/2 cluster are seen to project
ventrally. PPL1 cluster neurons are seen to project medially and dorsally towards the mushroom body, and the PPM3 neurons
are observed to project towards the central complex.
Approximately 127 Scer\GAL4[ple.PF] expressing cells can be identified in the adult protocerebrum. Scer\GAL4[ple.PF] is highly expressed in neurons that terminate in the vertical lobes of the mushroom body and in the lateral part of the mushroom
body horizontal lobes. There are also a few fibers wrapped around the distal tip of the mushroom body horizontal lobes.
Scer\GAL4[ple.PF] drives expression in the dopaminergic cluster PAL, PPL1, PPL2ab, PPM1, 10 neurons of the PPM2 cluster, 8 neurons of the PPM3
cluster and 13 neurons of the PAM cluster.
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| Marker for |
dopaminergic neuron
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| Reflects expression of | |||
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Progenitors & Descendants
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Comments
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Stocks Listed in FlyBase
( 1 )
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| Bloomington |
8848
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 External Crossreferences & Linkouts
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| Other Crossreferences | |||
| Linkouts | |||
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Synonyms & Secondary IDs
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| Reported As | |||
| Symbol Synonym |
(TH)-Gal4
(TH)-GAL4
DTHGal4
P{ple-GAL4.F}
P{TH-GAL4}
Pple(TH)-Gal4
PTH-GAL4
Ple-Gal4
ple-Gal4
TH1-Gal4
TH-G4
TH-Gal
TH-GAL4
(Yang, 2005, Sykes and Condron, 2005, Yang et al., 2005, Auluck et al., 2005, Menzies et al., 2005, Friggi-Grelin et al., 2003, Freeman et al., 2012, Lima and Miesenbock, 2005, Park et al., 2006, Cooper et al., 2006, Schneider et al., 2012, Claridge-Chang et al., 2009, Rival et al., 2011, Muñoz-Soriano and Paricio, 2007, Lavara-Culebras and Paricio, 2007, Palgi et al., 2009, Park et al., 2009, Corl et al., 2009, Honjo and Furukubo-Tokunaga, 2009, Yang et al., 2006, Yang et al., 2008, Yang et al., 2008, Ishida et al., 2012, Chen et al., 2012, Sitaraman et al., 2008, Riemensperger et al., 2011, Tanaka et al., 2008, Lee et al., 2007, Lee et al., 2008, Makos et al., 2010, de Haro et al., 2010, Yu et al., 2010, Imai et al., 2010, Du et al., 2010, Mao and Davis, 2009, Chiang et al., 2011, Friggi-Grelin et al., 2003, Pauls et al., 2010, Giang et al., 2011, Kahsai et al., 2012, Wakabayashi-Ito et al., 2011, Pauls et al., 2010, Munoz-Soriano et al., 2012, Kaun et al., 2011, Aso et al., 2012, Jansen et al., 2009, Trinh et al., 2008, Kong et al., 2010, Barone et al., 2011, Xiong et al., 2012, Keleman et al., 2012)
TH-Gal4
(Riemensperger et al., 2005, Schwaerzel et al., 2003, Yuan et al., 2006, Wheeler et al., 2006, Schroll et al., 2006, Chen et al., 2012, Liu et al., 2007, Duran et al., 2012, Wang et al., 2011, Ganguly-Fitzgerald et al., 2006, Melcher and Pankratz, 2005, Imai et al., 2008, Kaplan et al., 2008, Lee et al., 2008, Al-Anzi et al., 2009, Venderova et al., 2009, Lee et al., 2008, Gao et al., 2008, Wu et al., 2010, Kim et al., 2012, Alekseyenko et al., 2010, Nässel et al., 2008, White et al., 2010, Chiang et al., 2011, Gehrke et al., 2010, Bang et al., 2011, Stavropoulos and Young, 2011, Liu et al., 2012)
TH-gal4
Th-gal4
TH Gal4
Th-Gal4
THGAL4
THGal4
tyrosine hydroxylase (TH)-Gal4
Tyrosine hydroxylase-GAL4
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| Secondary FlyBase IDs | |||
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References
( 100 )
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Recent research papers ( 29 )
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