FB2025_01 , released February 20, 2025
Recombinant Construct: P{BP.ARS1}
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General Information
Symbol
P{BP.ARS1}
FlyBase ID
FBtp0020145
Feature type
transgenic_transposable_element
Component Allele(s)
Size
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Description

Transgenic construct used to study the requirements for amplification of the third chromosome chorion gene cluster. Derived from P{BigParent} : the Ori-β origin of replication has been replaced with a 193bp fragment that includes the complete Saccharomyces cerevisiae ARS1 autonomously replicating sequence (SGD:S000029652).

Construct components
Component
Use(s)
Marker(s)
Component allele Cp18ch1
Transgenic product class
Component
Use(s)
Encoded product / tool
Description

Construct: 3.8kb SalI genomic fragment containing the Cp15 and Cp18 transcripts.

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
descendant(s)
Comments

construct_comment: P{BigParent} in which Ori66Dβ has been replaced by Scer\ARS1 sequence amplified using primers \'AGCTGGATCCCTAACAAAATAGCAAATTTCG\' and \'AGCTCTCGAGACAATCAATCAAAAAGCCAAA\'.

P{BigParent} in which Ori66Dβ has been replaced by Scer\ARS1 sequence amplified using primers \'AGCTGGATCCCTAACAAAATAGCAAATTTCG\' and \'AGCTCTCGAGACAATCAATCAAAAAGCCAAA\'.

Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Secondary FlyBase IDs
    References (2)