FB2025_01 , released February 20, 2025
Recombinant Construct: P{BP.ARS1}
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General Information
Symbol
P{BP.ARS1}
FlyBase ID
FBtp0020145
Feature type
transgenic_transposable_element
Component Allele(s)
Cp18ch1
Size
Expression Data
Associated insertion(s)
0 available
Molecular map
Description and Uses
Description

Transgenic construct used to study the requirements for amplification of the third chromosome chorion gene cluster. Derived from P{BigParent} : the Ori-β origin of replication has been replaced with a 193bp fragment that includes the complete Saccharomyces cerevisiae ARS1 autonomously replicating sequence (SGD:S000029652).

(Zhang and Tower, 2004)
Construct components
Component
Use(s)
Marker(s)
w+mC
Component allele Cp18ch1
Transgenic product class
Component
Use(s)
Encoded product / tool
Cp18
Description

Construct: 3.8kb SalI genomic fragment containing the Cp15 and Cp18 transcripts.

(Rubin and Spradling, 1982)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
P{BigParent}
(Zhang and Tower, 2004)
descendant(s)
Comments

construct_comment: P{BigParent} in which Ori66Dβ has been replaced by Scer\ARS1 sequence amplified using primers \'AGCTGGATCCCTAACAAAATAGCAAATTTCG\' and \'AGCTCTCGAGACAATCAATCAAAAAGCCAAA\'.

(Zhang and Tower, 2004)

P{BigParent} in which Ori66Dβ has been replaced by Scer\ARS1 sequence amplified using primers \'AGCTGGATCCCTAACAAAATAGCAAATTTCG\' and \'AGCTCTCGAGACAATCAATCAAAAAGCCAAA\'.

(Zhang and Tower, 2004)
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P{BP.ARS1}
(FlyBase, 1996-)
pBP-ARS1
(Zhang and Tower, 2004)
Secondary FlyBase IDs
    References (2)