Recombinant construct P{BP.Mini-1}

General Information
Symbol	P{BP.Mini-1}	FlyBase ID	FBtp0020207
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	0 available
Molecular map
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Zhang and Tower, 2004)
Uses	CV term Qualifiers & info Reference characterization ACE3 (Zhang and Tower, 2004) Ori66Dbeta (Zhang and Tower, 2004) Ori66Dβ (Zhang and Tower, 2004)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference regulatory/structural DNA (Zhang and Tower, 2004) selectable marker (Zhang and Tower, 2004)
Component Alleles
Allele	ACE3^mt-3
Reference(s)	(Zhang and Tower, 2004)
Molecular data	Construct: Residues 130-272 of ACE3³²⁰. (Zhang and Tower, 2004)
Phenotypic class
Phenotype manifest in
Other information

Allele	gypsy\su(Hw)BR⁴³⁰
Reference(s)	(Zhang and Tower, 2004)
Molecular data	Contains 12 su(Hw)-binding sites, corresponding to bp 647-1077 in the gypsy transposon. (Kuhn et al., 2003) Consists of a 430bp gypsy\su(Hw)BR, gypsy sequences 647-1077 (coordinates of FBrf0045014). (Roseman et al., 1995) Consists of a 430bp gypsy\su(Hw)BR, including all 12 binding sites, nucleotides 647 to 1077 of FBrf0045014. (Roseman et al., 1993) Construct: Consists of a 430bp gypsy\su(Hw)BR, including all 12 binding sites. (Patton et al., 1992)
Phenotypic class
Phenotype manifest in
Other information

Allele	Ori66Dβ^mt-2
Reference(s)	(Zhang and Tower, 2004)
Molecular data	Construct: Residues 1-560 of Ori66Dβ⁸⁸⁴. (Zhang and Tower, 2004)
Phenotypic class
Phenotype manifest in
Other information

Allele	w^+mC
Reference(s)	(Zhang and Tower, 2004)
Molecular data	Minigene of the white cDNA. (Chung et al., 1993) 4.15kb SpeI fragment of the w gene that carries a deletion of a large part of the first intron and the regulatory region is reduced to 300bp preceding the transcription start site. (Pirrotta, 1988) Construct: A 'mini-white' gene constructed by deletion of the HindIII-XbaI fragment from the long 5' intron. Carried by the Casper series of vectors.
Phenotypic class	courtship behavior defective \| male (Lloyd et al., 2002, Hing and Carlson, 1996) eye color defective (Soldatov et al., 1999, Hagstrom et al., 1996) eye color defective, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002) visible \| dominant (True et al., 1996) visible \| recessive (Roseman et al., 1993)
Phenotype manifest in	pigment cell (Soldatov et al., 1999, Hagstrom et al., 1996, Roseman et al., 1993, Dorer and Henikoff, 1994, Qian and Pirrotta, 1995) pigment cell, with Scer\GAL4^Act5C.PI, w^{dsRNA.Sym.Scer\UAS} (Giordano et al., 2002)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	P{BigParent} (Zhang and Tower, 2004)
descendant(s)
Comments
	construct_comment: P{BigParent} from which the entire genomic insert between the two gypsy\su(Hw)BR⁴³⁰ sequences has been deleted and replaced by ACE3^mt-3 fused to Ori66Dβ^mt-2. (Zhang and Tower, 2004) P{BigParent} from which the entire genomic insert between the two gypsy\su(Hw)BR⁴³⁰ sequences has been deleted and replaced by ACE3^mt-3 fused to Ori66Dβ^mt-2. (Zhang and Tower, 2004)
Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	P{BP.Mini-1} (FlyBase, 1996-) pMini-1 (Zhang and Tower, 2004)
Secondary FlyBase IDs

References ( 1 )
Research paper	Zhang and Tower, 2004, Development 131(9): 2089--2099 Sequence requirements for function of the Drosophila chorion gene locus ACE3 replicator and ori- origin elements. [FBrf0174575]