Recombinant construct P{UAS(-FRT)G-oα47A.GTP}

General Information
Symbol	P{UAS(-FRT)G-oα47A.GTP}	FlyBase ID	FBtp0020306
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	0 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Katanaev et al., 2005)
Uses	CV term Qualifiers & info Reference binary system (targeted) G-oalpha47A (Katanaev et al., 2005) characterization G-oalpha47A (Katanaev et al., 2005)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference binary component (Katanaev et al., 2005)
Component Alleles
Allele	Gαo^GTP.Scer\UAS
Reference(s)	(Katanaev et al., 2005, Katanaev and Tomlinson, 2006, Schwabe et al., 2005, Yoshiura et al., 2012, Dahdal et al., 2010, Yao and Carlson, 2010)
Molecular data	The Scer\FRT cassette has been excised, juxtaposing the Scer\UAS regulatory sequences and mutant form of the G-oα47A class II isoform. Encodes a form of G-oα47A which is maintained in the GTP bound state. (Katanaev et al., 2005)
Phenotypic class	cell polarity defective, with Scer\GAL4^pnr-MD237 (Katanaev and Tomlinson, 2006) circadian rhythm defective, with Scer\GAL4^P0.5.Pdf (Dahdal et al., 2010) neuroanatomy defective, with Scer\GAL4^repo (Schwabe et al., 2005) planar polarity defective, with Scer\GAL4^pnr-MD237 (Katanaev and Tomlinson, 2006) planar polarity defective \| cell autonomous, with Scer\GAL4^Bx-MS1096 (Katanaev et al., 2005)
Phenotype manifest in	eo glial cell, with Scer\GAL4^sca-537.4 (Katanaev and Tomlinson, 2006) eo glial cell \| ectopic, with Scer\GAL4^sca-537.4 (Katanaev and Tomlinson, 2006) eo neuron, with Scer\GAL4^pnr-MD237 (Katanaev and Tomlinson, 2006) eo neuron \| ectopic, with Scer\GAL4^pnr-MD237 (Katanaev and Tomlinson, 2006) eo sensory structure, with Scer\GAL4^pnr-MD237 (Katanaev and Tomlinson, 2006) eo sensory structure, with Scer\GAL4^sca-537.4 (Katanaev and Tomlinson, 2006) eo sensory structure \| ectopic, with Scer\GAL4^pnr-MD237 (Katanaev and Tomlinson, 2006) macrochaeta, with Scer\GAL4^pnr-MD237 (Katanaev and Tomlinson, 2006) mechanosensory sensory organ, with Scer\GAL4^pnr-MD237 (Katanaev and Tomlinson, 2006) medial triple row, with Scer\GAL4^pnr-MD237 (Katanaev and Tomlinson, 2006) scolopidial neuron, with Scer\GAL4^sca-537.4 (Katanaev and Tomlinson, 2006) scolopidial neuron \| ectopic, with Scer\GAL4^sca-537.4 (Katanaev and Tomlinson, 2006) sensory mother cell, with Scer\GAL4^sca-537.4 (Katanaev and Tomlinson, 2006) sensory organ cell, with Scer\GAL4^pnr-MD237 (Katanaev and Tomlinson, 2006) sensory organ cell \| ectopic, with Scer\GAL4^pnr-MD237 (Katanaev and Tomlinson, 2006) socket, with Scer\GAL4^pnr-MD237 (Katanaev and Tomlinson, 2006) socket, with Scer\GAL4^sca-537.4 (Katanaev and Tomlinson, 2006) socket \| ectopic, with Scer\GAL4^pnr-MD237 (Katanaev and Tomlinson, 2006) socket \| ectopic, with Scer\GAL4^sca-537.4 (Katanaev and Tomlinson, 2006) thecogen cell, with Scer\GAL4^sca-537.4 (Katanaev and Tomlinson, 2006) thecogen cell \| ectopic, with Scer\GAL4^sca-537.4 (Katanaev and Tomlinson, 2006) wing hair \| cell autonomous \| supernumerary, with Scer\GAL4^Bx-MS1096 (Katanaev et al., 2005) wing margin bristle, with Scer\GAL4^Bx-MS1096 (Katanaev and Tomlinson, 2006) wing margin bristle, with Scer\GAL4^pnr-MD237 (Katanaev and Tomlinson, 2006)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)
descendant(s)
Comments

Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	Go-GTP (Schwabe et al., 2005) P{UAS(-FRT)G-oα47A.GTP} (FlyBase, 1996-) UAS-Go^GTP (Katanaev and Tomlinson, 2006) UAS-Go-GTP (Katanaev et al., 2005, Dahdal et al., 2010) UAS-Gα_o^GTP (Yao and Carlson, 2010)
Secondary FlyBase IDs

References ( 6 )
Research paper	Yoshiura et al., 2012, Dev. Cell 22(1): 79--91 Tre1 GPCR Signaling Orients Stem Cell Divisions in the Drosophila Central Nervous System. [FBrf0217269] Dahdal et al., 2010, Neuron 68(5): 964--977 Drosophila Pacemaker Neurons Require G Protein Signaling and GABAergic Inputs to Generate Twenty-Four Hour Behavioral Rhythms. [FBrf0212523] Yao and Carlson, 2010, J. Neurosci. 30(13): 4562--4572 Role of G-proteins in odor-sensing and CO2-sensing neurons in Drosophila. [FBrf0210441] Katanaev and Tomlinson, 2006, Proc. Natl. Acad. Sci. U.S.A. 103(17): 6524--6529 Dual roles for the trimeric G protein Go in asymmetric cell division in Drosophila. [FBrf0191444] Katanaev et al., 2005, Cell 120(1): 111--122 Trimeric G protein-dependent frizzled signaling in Drosophila. [FBrf0183772] Schwabe et al., 2005, Cell 123(1): 133--144 GPCR signaling is required for blood-brain barrier formation in Drosophila. [FBrf0189922]