Recombinant construct P{Hsp70Ba.Δ}

General Information
Symbol	P{Hsp70Ba.Δ}	FlyBase ID	FBtp0020615
Feature type	transgenic_transposon

Size		Expression data
Associated insertions	1 available
Molecular map
Recent Updates
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FB2013_03
FB2013_02
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Description & Uses
Species	synthetic construct
Location-dependent role
Description	CV term Qualifiers & info Reference transposon P-element (Gong and Golic, 2004)
Uses	CV term Qualifiers & info Reference targeted recombination donor Hsp70Ba (Gong and Golic, 2004)
Cloning Sites	Location Restriction sites Reference
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Extent
Segments & Size
Total Size
Left end
Right end

Segments	Number Orientation Symbol
Reference
Features
	CV term Qualifiers & info Reference
Component Alleles
Allele	w^+mW.hs
Reference(s)	(Gong and Golic, 2004)
Molecular data	A mini-w gene is expressed under the control of an Hsp70 promoter. (Heinrich et al., 1993) w gene expression is under the influence of FLIP recombinase. (Golic and Lindquist, 1989) Expression of a fragment of w genomic DNA with a 2.2kb deletion of 3' flanking sequences and a reduction in the size of the largest intron to 356bp is driven by a 388bp fragment of an Hsp70 gene. (Klemenz et al., 1987) Construct: A 'mini-white' gene constructed by deletion of the XbaI-HindII fragment from the long 5' intron. Carried by the W6 and W8 series of vectors.
Phenotypic class	suppressor of variegation, with Su(var)205⁵⁰² (Ahmad and Golic, 1996)
Phenotype manifest in	pigment cell (Kurkulos et al., 1994, Golic and Lindquist, 1989)
Other information
Expression Data
Reporter Expression

Additional Information
Statement Reference

Marker for
Reflects expression of
Progenitors & Descendants
progenitor(s)	pP{W25} (Gong and Golic, 2004)
descendant(s)
Comments
	construct_comment: A 4.1kb genomic fragment corresponding to the region just proximal to Hsp70Ba (nucleotides 103592-107652 of BACR28I14) has been inserted into pP{W25} using NotI and Acc65I sites. A 4.3kb genomic fragment corresponding to the region just distal to Hsp70Ba (nucleotides 109380-113666 of BACR28I14) has been inserted into pP{W25} using AscI and BsiWI sites.Donor ends-out targeting construct which is designed to result in deletion of the Hsp70Ba gene in a targeting event. (Gong and Golic, 2004) A 4.1kb genomic fragment corresponding to the region just proximal to Hsp70Ba (nucleotides 103592-107652 of BACR28I14) has been inserted into pP{W25} using NotI and Acc65I sites. A 4.3kb genomic fragment corresponding to the region just distal to Hsp70Ba (nucleotides 109380-113666 of BACR28I14) has been inserted into pP{W25} using AscI and BsiWI sites. (Gong and Golic, 2004) Donor ends-out targeting construct which is designed to result in deletion of the Hsp70Ba gene in a targeting event. (Gong and Golic, 2004)
Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs
Reported As
Symbol Synonym	Hsp70Ba deletion construct (Gong and Golic, 2004) P{Hsp70Ba.Δ} (FlyBase, 1996-)
Secondary FlyBase IDs

References ( 1 )
Research paper	Gong and Golic, 2004, Genetics 168(3): 1467--1476 Genomic deletions of the Drosophila melanogaster Hsp70 genes. [FBrf0180286]